Flourimetric and colorimetric determination of 1-amino-2-propanol, ethanolamine, benzocaine and 4-amino benzoic acid

A convenient and simple fluorimetric method for the determination of 1-amino-2-propanol, ethanolamine, benzocaine and 4-amino benzoic acid has been developed. The method depends on the reaction of the previously mentioned amines with m-hydroxy benzaldehyde to form highly fluorescent Schiff bases in absolute alcohol at 70C for 20 minutes. On the other hand, these primary amine-containing compounds were determined by colorimetric method. The Schiff bases obtained possess yellow with maximum absorption wave length range of 460-480 nm. Beer's law obeyed in the range of 0.1-5 mg/ml. The effect of pH, temperature, time and solvent of the reaction for both fluorimetric and colorimetric methods were studied and optimized