ABSTRACT
An unusually high incidence of microcephaly in newborns has recently been observed in Brazil. There is a temporal association between the increase in cases of microcephaly and the Zika virus (ZIKV) epidemic. Viral RNA has been detected in amniotic fluid samples, placental tissues and newborn and fetal brain tissues. However, much remains to be determined concerning the association between ZIKV infection and fetal malformations. In this study, we provide evidence of the transplacental transmission of ZIKV through the detection of viral proteins and viral RNA in placental tissue samples from expectant mothers infected at different stages of gestation. We observed chronic placentitis (TORCH type) with viral protein detection by immunohistochemistry in Hofbauer cells and some histiocytes in the intervillous spaces. We also demonstrated the neurotropism of the virus via the detection of viral proteins in glial cells and in some endothelial cells and the observation of scattered foci of microcalcifications in the brain tissues. Lesions were mainly located in the white matter. ZIKV RNA was also detected in these tissues by real-time-polymerase chain reaction. We believe that these findings will contribute to the body of knowledge of the mechanisms of ZIKV transmission, interactions between the virus and host cells and viral tropism.
Subject(s)
Humans , Male , Female , Infant, Newborn , Adult , Infectious Disease Transmission, Vertical , Microcephaly/virology , Viral Tropism/physiology , Zika Virus Infection/congenital , Zika Virus/physiology , Amniotic Fluid/virology , Brain/embryology , Brain/virology , Immunohistochemistry , Infant, Newborn , Placenta/virology , Pregnancy , RNA, Viral/analysisSubject(s)
Humans , Female , Pregnancy , Infant, Newborn , Infant , Algorithms , Cytomegalovirus , Cytomegalovirus Infections/diagnosis , Pregnancy Complications, Infectious , Toxoplasmosis, Congenital/diagnosis , Clinical Laboratory Techniques , Congenital Abnormalities , Fetus , Cytomegalovirus Infections/complications , Cytomegalovirus Infections/transmission , Amniotic Fluid/virology , Sensitivity and Specificity , Seroepidemiologic Studies , Toxoplasmosis, CongenitalABSTRACT
OBJETIVO: Determinar la utilidad de la concentraciónde glucosa en líquido amniótico en pacientes con trabajo de parto pretermino, como factor predictivo de infección intraamniótica. MATERIAL Y METODOS: Se practicó amniocentesis a 56 gestantes con trabajo de parto pretérmino y membranas íntegras, enviándose muestra para Gram, cultivo y concentración de glucosa. Simultáneamente se obtuvieron muestras de líquido amniótico en 62 pacientes a quienes se les indicó amniocentesis por otras patologías y que sirvieron de grupo de control. Se correlacionaron los resultados con la presencia de parto pretérmino y/o evidencia clínica de corioamnionitis utilizando métodos estadisticos de Chi cuadrado y "t" test. CONCLUSION: La determinación de glucosa en líquido amniótico es una alternativa diagnóstica rápida y certera en la identificación de infección intraamniótica en pacientes con actividad uterina pretérmino(Truncado 2500 caracteres)