ABSTRACT
INTRODUÇÃO: Angiostrongylus vasorum é um nematóide que parasita cães domésticos e eventualmente o homem. MÉTODOS: O objetivo deste trabalho foi observar a atividade predatória in vitro do extrato bruto enzimático do fungo Duddingtonia flagrans sobre larvas de primeiro estádio A. vasorum em condições laboratoriais no meio ágar-água 2 por cento. RESULTADOS: Ao final do experimento, os percentuais de redução das L1 de A. vasorum observados foram de: 53,5 por cento (24h) e 71,3 por cento (48h) CONCLUSÕES: O extrato bruto enzimático do fungo D. flagrans destruiu in vitro as L1, podendo ser utilizado como controle biológico desse nematóide.
INTRODUCTION: Angiostrongylus vasorum is a nematode parasite of domestic dogs and potentially of humans. METHODS: This study aimed to observe the predatory activity in vitro of a crude enzyme extract of the fungus Duddingtonia flagrans on first-stage larvae of A. vasorum in laboratory conditions on 2 percent water-agar. RESULTS: At the end of the experiment, the percentage reductions observed for A. vasorum L1 were 53.5 percent (24h) and 71.3 percent (48h). CONCLUSIONS: Crude enzyme extract of the fungus D. flagrans destroyed the L1 in vitro and can be used as a biological control for this nematode.
Subject(s)
Animals , Dogs , Angiostrongylus/drug effects , Ascomycota/chemistry , Complex Mixtures/pharmacology , Pest Control, Biological/methods , Angiostrongylus/growth & development , Larva/growth & development , Larva/microbiologyABSTRACT
The effects of neuropharmacological agents on the motility of irradiated and non-irradiated Angiostrongylus cantonensis adult females were studied. GABA induced complete paralysis in non-irradiated and 5,000 R-irradiated worms, but caused only slight paralysis on 10,000 R-irradiated worms. The paralytic effect of GABA was antagonised by picrotoxin. The reason for low susceptibility of heavily irradiated worms to GABA is not known. There was no difference in susceptibility of non-irradiated and irradiated worms to other neuropharmacological agents including eserine, phenylephrine and dibenamine.