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Rev. colomb. biotecnol ; 11(2): 114-126, dic. 2009.
Article in Spanish | LILACS | ID: lil-550525

ABSTRACT

El objetivo de esta investigación consistió en comparar la flora microbiana a partir de la metodología convencional implementada en Garagoa, Boyacá, para el tratamiento de residuos sólidos domiciliarios con una metodología experimental que consistió en humectar los residuos con agua en lugar de lixiviado, y no mezclar el material por tratar con otro de distintos tiempos de compostaje; para tal fin, se caracterizaron y determinaron los tamaños poblacionales de heterótrofas totales, pseudomonas, hongos y actinomicetos a partir de muestras tomadas en distintas etapas del proceso. De las distintas muestras analizadas se identificaron hongos de los géneros Cladosporium y Aspergillus, propios del proceso de compostaje, Fusarium, Scopulariopsis, Moniliella, Curvularia y levaduras del género Rhodothorula.En el agar ISP2 (para el aislamiento de actinomicetos) se identificaron diversos morfotipos: bacilos esporulados, bacilos gramnegativos, cocobacilos, cocos grampositivos y una bacteria del género Streptomyces. De acuerdo con los resultados de los tamaños poblacionales de los grupos de microorganismos estudiados no se presentaron diferencias significativas entre los tratamientos evaluados; sin embargo, el mayor promedio de los recuentos se presentó en el tratamiento experimental.


This research was aimed at comparing microorganisms from conventional household solid waste treatment in Garagoa, Boyacá, using an experimental methodology consisting of moistening waste with water instead of lixiviate and not mixing the material to be treated with other material having different composting times (i.e. organic fertilizer). Complete heterotopous, pseudomona, fungi and actinomycete population size were thus characterised from samples taken from different stages of the process. Fungi from the Cladosporium and Aspergillus genera (from composting) were identified from this analysis, as well as Fusarium, Scopulariopsis, Moniliella, Curvularia and yeasts from the Rhodothorula genera. Some morphotypes were identified in the ISP2 agar (for the actinomycete isolate): sporulated bacilli, gram-negative bacilli, cocobacilli, gram-positive coci and one bacterium from the Streptomyces genus. According to the results regarding the population sizes of the microorganism groups studied, no meaningful differences were presented between the treatments being tested; however, the experimental treatment presented the highest average count.


Subject(s)
Antigens, Fungal/adverse effects , Antigens, Fungal/chemistry
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