ABSTRACT
Spontaneous cell fusion induced by the bacterium Haemophilus paragallinarum has been recently reported as an alternative technique to generate hybridomas producing monoclonal antibody (mAb). In order to investigate the advantages of this technique to produce anti-tumor monoclonal antibodies we performed comparative experiments between H. paragallinarum induced spontaneous cell fusion and polyethylene glycol (PEG) mediated fusion. Hybridomas producing monoclonal antibodies to an experimental murine lymphoma antigen, the Dalton's lymphoma associated antigen (DLAA) were generated and their sensitivity and specificity were ascertained. The spontaneous fusion yielded more number of stable and specific hybridomas than PEG mediated fusion. The results suggest the advantage of H. paragalinarum induced cell fusion for the simplified production of specific antitumor monoclonal antibodies.
Subject(s)
Animals , Antibodies, Monoclonal/biosynthesis , Antibodies, Neoplasm/biosynthesis , Antigens, Neoplasm/biosynthesis , Cell Fusion , Enzyme-Linked Immunosorbent Assay , Haemophilus , Histocompatibility Antigens Class I/biosynthesis , Immunoglobulin G/biosynthesis , Immunoglobulin M/biosynthesis , Lymphoma/immunology , Mice , Mice, Inbred DBA , Sensitivity and SpecificityABSTRACT
Las técnicas modernas en el campo inmunológico y de biología molecular han significado una revolución en el campo biomédico al permitir la detección, reconocimiento y manipulación biológica de antígenos presentes en órganos, tejidos y células especializadas. Se discute la importancia médica de la expresión de diversos antígenos que incluyen marcadores de superficie, productos solubles, citoquinas e isoenzimas con énfasis en la comparación de marcadores con la expresión antigénica de tejidos normales a nivel embrionario y postnatal
Subject(s)
Humans , Male , Female , Antigens, Neoplasm/analysis , Antigens, Neoplasm/biosynthesis , Antigens, Neoplasm/classification , Antigens, Surface , Biomarkers, TumorABSTRACT
Hormonal modulation of in vitro biosynthesis of three prostatic secretory proteins, prostate specific acid phosphatase (PSAP), prostate specific antigen (PSA) and prostatic inhibin peptide (PIP) by human benign hyperplasia (BPH) tissue was studied. LH and inhibins caused increase in the synthesis of all three proteins whereas FSH enhanced the synthesis of PIP and PSA only but decreased PSAP synthesis. Prolactin and thyroid releasing hormone decreased synthesis of PIP and PSAP. However, PSA synthesis was enhanced by TRH and was decreased by prolactin. Estradiol caused significant increase in PSA and PSAP but no discernible changes in PIP synthesis were noticed. Testosterone caused an increase in PIP, PSA and PSAP. These data indicate that biosynthesis of PIP, PSA and PSAP by BPH tissue is under multihormonal regulation.
Subject(s)
Acid Phosphatase/biosynthesis , Antigens, Neoplasm/biosynthesis , Estradiol/pharmacology , Follicle Stimulating Hormone/pharmacology , Hormones/pharmacology , Humans , Inhibins/biosynthesis , Luteinizing Hormone/pharmacology , Male , Prostate/metabolism , Prostate-Specific Antigen , Testosterone/pharmacology , Thyrotropin-Releasing Hormone/pharmacology , Biomarkers, Tumor/biosynthesisABSTRACT
Trehalose dimycolate, a mycobacterial glycolipid also known as cord factor, retains some of the antitumor properties of the intact BCG. Murine macrophages incubated in vitro in the presence of trehalose dimycolate for 20 h at 37-C released a factor which was cytotoxic for the L929 tumor cell line. Anti-tumor necrosis factor (TNF) blocked the cytotoxicity of trehalose dimycolate macrophage supernatant. Therefore the antitumor activity of trehalose dimycolate can be attributed to TNF released in the in vitro assay