Effects of atropine and propranolol on lung inflammation in experimental envenomation: comparison of two buthidae venoms

Previous works had shown that scorpion venom induced neurotransmitter elevation and an inflammatory response associated with various anatomo-pathological modifications. The most dangerous scorpions species in Algeria responsible for these effects are Androctonus australis hector(Aah) and Androctonus amoreuxi (Aam). Results Comparison of the physiopathological effects induced by the two venoms showed differences in the kinetic of cytokine release and in lung injury. The lung edema was only observed in response to Aah venom and it was correlated with cell infiltration. In order to better understand the involved mechanism in inflammatory response, we used two antagonists, atropine (non-selective muscarinic antagonist) and propranolol (β adrenergic antagonist), which lead to a decrease of cell infiltration but has no effect on edema forming.Conclusion These results suggest another pathway in the development of lung injury following envenomation with Aam or Aah venom.

Arritmias complexas que surgem durante o ecocardiograma sob estresse com dobutamina e atropina / Complex arrythmias that occur during dobutamine-atropine stress echocardiography

Objetivo: Analisar arritmias complexas (AC) que surgem durante o ecocardiograma sob estresse com dobutamina(EED) e atropina. Método: Dentre 6563 EED, analisamos, retrospectivamente, o grupo A (GA) de 167 (2,5 por cento) pacientes(pac) com AC(maior ou igual 3 ectopias consectivas) que surgiram durante o EED e comparamos ao grupo controle (GC) de 167 pac sem ectopia. Classificamos AC em taquicardia supraventricular (TSV), ventricular não-sustentada (TVNS) e fibrilação atrial (FA). Usamos dobutamina (com ou sem atropina) em até 4 estágios. Resultados: Cada grupo compunha-se de 94 mulheres e 114 pac maior ou igual 65 anos. Foram maiores no GA a pressão arterial (PA)sistólica basal (139 mais ou menos 20 vs 132 mais ou menos 15; p igual 0,003) e, no EED (151 mais ou menos 25 vs 143 mais ou menos 19; p igual 0,004), a PA diastólica...

Spectrophotometric determination of atropine sulphate

Atropine reacts with sodium nitrite and 1-naphthylamine when heated from 95 -100°C giving an orange color with a maximum absorbance at 464 nm. This reaction is greatly affected by the pH of the solution and the time of heating. The assay obeys Beer's law in concentrations ranging from 10-800 micro g/ml and the visual limit of detection [LOD] is 0.5 micro g/ml. It has been postulated that obidoxime chloride may interfere with this reaction if co exists with atropine sulphate in the same solution or preparation, as this mixture is widely used for the treatment of organo-phosphorus pesticides poisoning