ABSTRACT
The current investigation was conducted aiming at throwing some light on the deteriorative effect of gamma-irradiation on the biochemical and hematological parameters as well as the tissues of liver and kidney of male albino rats. Furthermore, the study dealt with increasing the protectivity of rats against gamma-irradiation through examining the curative effect of some antioxidants besides the ethanolic extract of beans hull [phenolic compounds]. The ethanolic extract of beans hull represented 13.02% of the weight of hulls, while the total phenolic content represented 26.45 micro g/g of beans hull weight. Results showed that the ethanolic extract of beans hull contains some important compounds include; iso-butyl protocatecolate, beta-carotene, flavonoid glycoside and alpha-tocopherol which are effective as antioxidants. Results indicated that the whole body gamma-irradiation of rats at a dose of [6 Gy] caused significant diminish in haemoglobin, [RBC's] count, serum albumin and glutathione enzyme in serum. On the other hand, irradiation led to significant increases in total lipids, cholesterol, glucose, lipoproteins, LDL-cholesterol, urea and creatinine. Likewise, gamma-irradiation caused significant elevation in the activity of liver enzymes [ALT, AST] and kidney function tests [creatinine and urea]. The histological examination showed some changes in liver and kidney tissues. Giving antioxidants to rats improved significantly blood antioxidant markers and liver enzymes, besides of the kidney and liver functions. The ethanolic extract of beans hull given to the rats at a dose of 40 micro g/100g b.w. showed a positive effect on biochemical parameters and also on histological ultrastructure compatable with the melatonin effect