Application research of protein test by using biuret reagent / 中国医疗器械杂志

<p><b>OBJECTIVE</b>To investigate the biuret reagent to detect proteins in the application, the impact of different test conditions for test results.</p><p><b>METHODS</b>The biuret method to select three different instruments, reagents, calibrators are arranged in combination to form 27 sets of detection systems, each detection system is a combination of 5 serum samples for testing, 5 measured values obtained, the selection process normality good a serum for the study to determine the mean value of all AST after culling outliers obtained in order to calculate the various detection systems use a combination of biuret reagent to detect proteins bias.</p><p><b>RESULTS</b>The use of different detection equipment to detect proteins biuret reagent bias, homogeneity of variance (P = 0.467), the difference was not statistically significant (F = 1.688, P = 0.421). different detection reagents using biuret reagent to detect proteins bias, homogeneity of variance (P = 0.574), a statistically significant difference (F = 5.784, P = 0.011). different calibrators use biuret reagent to detect proteins bias, homogeneity of variance (P = 0.467), the difference was statistically significant (F = 5.289, P = 0.000).</p><p><b>CONCLUSION</b>Biuret reagent in the detection of protein applications, impact detection reagents and calibrators will test result, during the test than when it is necessary to detect deviation detection reagents and calibrators due to be considered.</p>

Characteristics of protein from red crab (Chionoecetes japonicus) shell by commercial proteases

This study was performed to examine the characteristics of protein of red crab (Chionoecetes japonicus) shell powder hydrolyzed by commercial proteases. Red crab shell was digested by commercial proteases, such as Protamex (P), Neutrase (N), Flavourzyme (F), Alcalase (A), Protease M (PM) and Protease A (PA). Protein yield analyzed by Biuret assay, absorbance at 280 nm and brix revealed that PA was the enzyme having the highest proteolytic activity. SDS PAGE showed that molecular weight of proteins produced by protease treatments was various and below 150 kDa. Combinational treatment of proteases (PA + P, PA + PM, PA + F, PA + A) was tried whether these increase protein hydrolysis from red crab shell powder compared to a PA single treatment. Soluble protein content was similar, but amino acid concentration by combinational treatments was higher than PA single treatment [PA + P 247.4 mg/g > PA + F (206.4 mg/g) > PA + A (133.4 mg/g) > PA + PM (59.1 mg/g) > PA (54.9 mg/g)]. Amino acid composition by combinational treatments was slightly different. Most abundant essential amino acids were phenylalanine, glycine, alanine, and leucine, whereas tyrosine and cystine were not detected.

Perfil eletroforético das proteínas e concentraçäo das imunoglobulinas plasmáticas em pacientes assintomáticos e sintomáticos portadores de HIV / Proteins electrophoretic profile and immunoglobulins plasmatics concentration in asymptomatics and symptomatics patients infected by HIV

Estudos têm demonstrado que 81 por cento dos pacientes infectados pelo HIV apresentam um padräo proteico anormal devido ao estado hipermetabólico. Os achados laboratoriais revelam que as anormalidades da imunidade humoral precedem a imunidade mediada pelas células e portanto, o diagnóstico da infecçäo pelo HIV depende do estabelecimento da infecçäo definindo o estado imunológico e o quadro clínico do paciente que ocorre secundário à infecçä e à perda da funçäo imune. No presente trabalho, realizamos um estudo comparativo do perfil eletroforético das proteínas, bem como a determinaçäo da concentraçäo das imunoglobulinas plasmáticas em pacientes portadores de HIV (assintomáticos e sintomáticos) com o objetivo de investigarmos alteraçöes específicas das fraçöes protéicas (albumina e globulina) durante os diferentes estágios da doença. Foram analisadas 30 amostras de sangue coletado de pacientes HIV positivos divididos em dois grupos (assintomáticos e sintomáticos). As proteínas totais foram determinadas pelo método do Biureto, sendo observada uma hipoproteinemia em 60 por cento dos pacientes sintomáticos e valores normais em todos os assintomáticos. A eletroforese das proteínas foi realizada em gel de agarose mostrando hipoalbuminemia em 70 por cento dos pacientes assintomáticos e 90 por cento sintomáticos. A hipergamaglobulinemia predominou em 100 por cento dos sintomáticos e 90 por cento dos assintomáticos. As fraçöes alfa e beta globulinas apresentaram-se normais nos dois grupos. As imunoglobulinas foram avaliadas por turbidimetria e uma elevaçäo significativa foi verificada na concentraçäo de IgG em todos os pacientes sintomáticos. Por outro lado, os valores de IgA e IgM apresentaram-se dentro dos valores de referência. Os resultados estäo de acordo com vários trabalhos citados na literatura, comprovando que a AIDS é uma gamopatia monoclonal, caracterizada por hipoalbuminemia e hipergamaglobulinemia. O trabalho mostra portanto, que, a avaliaçäo de parâmetros laboratoriais mais simples säo úteis no monitoramento da síndrome em estudo, como também fortes indicadores do estágio da doença.

Glaucoma facolítico: correlaçäo clínica com proteínas no humor aquoso / Phacolytic glaucoma: clinical correlation with aqueous humor proteins

Säo estudados 5 casos com diagnóstico clínico de glaucoma facolítico do Hospital das Clínicas da Faculdade de Medicina de Botucatu - UNESP, que foram submetidos à facectomia. Foram dosadas as proteínas totais do humor aquoso (H.A.) destes pacientes e feito exame anatomopatológico do cristalino e citológico do H.A. Os autores discutem a correlaçäo dos achados dos exames com o quadro clínico e chamam atençäo para a facilidade e o baixo custo do teste do biureto para se dosar proteínas no H. A.

Ammonification and nitrification of biuret in soils at different moisture levels

In laboratory experiments under three moisture levels [50, 200 and 400% whc] with sandy, sandy loam and clay loam soils, the ammonification and subsequent nitrification of 200 ppm biuret-N was studied. Ammonification was more rapid in heavy than in light textured soils. Mineralization percentages of biuret-N were 25, 27 and 30.2 at 400% whc, in sandy, sandy loam and clay loam soil, respectively, and most mineralized-N was in the form of NH4 +. The corresponding figures at 50% whc were 15, 16 and 21% and most nitrogen was in the NO3 form. NO-2-N accumulated in considerable amount only at 200% whc, and persisted longer in soils which reflect the relative sensitivity of Nitrobacter to anaerobic conditions. Whc, of 400%, inhibited both Nitrosomonas and Nitrobacter and no NO-2 or NO-3 was detected in the soils. Biuret, by itself, at 200 ppm-N had no effect on nitrifying bacteria

Effect of UV Irradiation on Superoxide Dismutase Activities of the Rabbit Skin / 대한피부과학회지

This study was undertaken to know whether the UV-irradiation of the skin causes changes in the superoxide dismutase(SOD) activities. After shaving, the back skin of the rabbit was irradiated with UV light ranging from 280 to 320 nm of wavelengths from Burdick lamb (UV800) in doses of either 0.5, 1.0 or l.5 J/cm2. The skin was removed imrnediately after irradiation and the enzyme activity was assayed by the method of McCord ad Fridovich (xanthine-xanthie oxidase system), One unit of the SOD activity was defined as the amount of the enzyme required to inhibit the rate of reduction of cytochrome c by 50%. The protein content of the enzyme was determined by biuret method. The SOD activity of the skin irradiated with 1.0 J/cm was 7. 78 + 1.62 unit/mg protein(Mean+SD; n=10), significantly higher than that of the control(nonirradiated) group(5.62+1.57 unit/mg protein; n=l0). No significant changes were found in the skins irradiated with 1.5 and 1.5 J/cm. This finding indicates that UV irradiation is capablc of increasing the SOD activitiea in the rabbit skin, and suggests that increased superoxide formed by UV irradiatiun induces the increased SOD activies.

A Study of Serum Albumin, Globulin, Total Protein, and A/G Ratio in Korean Mothers and Newborn Infants

With the availability of the method of analysis of serum protein using minute amounts of material, it was felt desirable to understand the protein metabolism and physiologic function in the body. The present study was undertaken to clarify the serum albumin, globulin and total protein at term to demonstrate the normal concentration and correlation between the 30 mother and newborn infant pairs. Serum albumin, globulin and total protein were determined by the Biuret method with pooled human serum. The A/G ration was calculated by formula of A/G. The following result were obstained. 1) In comparing the newborn infants of nonanemic mothers a albumin and total protein concentrations were higher and globlin concentrations decreased in the anemic mothers. 2) In comparing the nonanemic mothers and anemic mothers the mean albumin concentrations were nearly equal but globulin and total protein were slightly increased in the nonanemic mothers. 3) The mean serum albumin(of maternal and umbilical cord blood) was 3. 8+/-0. 35 gm/100 ml and 3. 8+/-0. 49 gm/100 ml respectively. 4) The mean serum globulin of mate. nal and umbilical cord blood was 2. 7+/-0. 41 gm/100 ml and 2. 32+/-0. 47 gm/100 ml respectively. The correlation of the globulin status between mot-hers and their newborn infants was not significant(r=0. 32). 5) The m-an serum total protein of maternal and umbilical cord blood was 6. 59+/-0. 59 gm/100 ml and 6. 02+/-0.57gm/100ml respectively.

Synovial Fluid Analysis as a Diagnostic Aid in Arthritis / 대한정형외과학회잡지

Extensive knowlege of the characteristics of synovial fluid has been available for at least the past 30 years, when a Monograph on the subject by Kling first appeared in 1938. Since that time, Ropes, Bauer(1953) and Hollander (1960, 1961, 1965) have published classic. Monographs on their extensive studies and findings of synovial fluid. Specific laboratory tests for diagnosis of various forms of arthritis are usually lacking. For example, the test for the rheumatoid factor in serum may be helpful in establishing the diagnosis of rheumatoid arthritis, but these are often negative in early cases and L. E. phenomenon is often negative in the early stage or between severe exacerbations of the Systemic lupus erythematosus. It has become increasingly clear during the past 10 years that synovial fluid analysis is both the most valuable and yet the most neglected differential diagnostic test for arthritis. Studies of synovial fluid have presented a virtually unexplored frontier in the investigation of arthritis. So, we studied the synovial fluid from 100 cases of various forms of arthritis in the Department of Orthopedic Surgery, Severance Hospital from May, 1968 to May, 1969. 100 cases of arthritis are; 30 cases of Osteoarthritis, 20 cases of Traumatic athritis, 25 cases of Rheumatoid arthritis, 10 cases of Septic arthritis, 5 cases of Tuberculous arthritis, and 10 cases of Non-specific bursitis. The synovial fluid were aspirated from the involved joints in aseptic conditions and follwing studies were done. 1) General appearance. 2) Mucin content by Acetic acid PPT. or Ropes test. 3) Viscosity by Drop test. 4) Cell count by Wright s stain. WBC: Total and differential count. RBC count. 5) Synovial sugar by Folin Wu method. 6) Fasting blood sugar by Folin Wu method. 7) Sugar difference between synovial sugar and Fasting blood sugar. 8) Total protein by Kingsley s Biuret method. 9) Bacterial culture in Septic arthritis. 10) Microscopic examination. RA cells by Sternheimer-Malbin stain in Rheumatoid arthritis. Cartilage fragments with simple wet preparations in Osteoarthritis. 6 kinds of arthritides were grouped into 3 categories based on the degree of inflammation of the synovial membrane as reflected by synovial fluid changes according to Ropes and Bauer s classification(1953). The first group, consisting of Osteoarthritis and Traumatic arthritis, was associated with mild inflammatory reactions and increased amount of fluid, but no significant changes in the number of WBC, sugar concentration, or quality of mucin. The second group was characterized by more sever inflammation of the synovial membrane and included Rheumatoid arthritis, Septic arthritis and Tuberculous arthritis. The second group was associated with decreased mucin content, increased WBC, polymorphonuclear leucocytes, RBC and protein and decreased amount of synovial sugar. RA cells were found in all cases of Rheumatoid arthritis and cartilage fragments in Osteoarthritis under the light microscope. The third group, an intermediate group-Non specific bursitis might have some distinguishing characteristics of synovial fluid but these were not usually diagnostic.