Infection by Henneguya sp. (Myxozoa) in the bone tissue of the gill filaments of the Amazonian catfish Hypophthalmus marginatus (Siluriformes) / Infecção por Henneguya sp. (Myxozoa) em tecido ósseo do filamento branquial do bagre amazônico Hypophthalmus marginatus (Siluriformes)

This study describes aspects of the infection caused by the myxosporean genus Henneguya, which forms cysts in the bony portion of the gill filaments of Hypophthalmusmarginatus. Specimens of this catfish were acquired dead from artisanal fishermen near the town of Cametá, state of Pará, northern Brazil, between July 2011 and May 2012. They were transported in refrigerated containers to the Carlos Azevedo Research Laboratory at the Federal Rural University of Amazonia, in Belém, where analyses were performed. After confirmation of parasitism by the genus Henneguya, observation were made using optical and differential interference contrast (DIC) microscopy. The histological technique of embedment in paraffin was used. Ziehl-Neelsen staining was applied to the histological sections. Necropsy analyses on specimens of H. marginatus showed that 80% of them (40/50) had cysts of whitish coloration inside the bony portion of the gill filaments, filled with Henneguya spores. The present study found inflammatory infiltrate in the vicinity of the cysts. Furthermore, the special Ziehl-Neelsen staining technique made it possible to mark the Henneguya sp. cysts in the bone tissue and in spore isolates in the gill tissue structure. The descriptions of these histopathological findings show that this parasite is very invasive and causes damage to its host tissues.

O presente estudo descreve os aspectos da infecção causada por mixosporídio do gênero Henneguya, formando cistos na porção óssea dos filamentos branquiais de Hypophthalmus marginatus. Espécimes desse bagre foram adquiridos mortos de pescadores artesanais perto da cidade de Cametá, Estado do Pará, Brasil, entre julho de 2011 e maio de 2012. Os animais foram transportados em contêineres refrigerados até o Laboratório de Pesquisa Carlos Azevedo, na Universidade Federal Rural da Amazônia, em Belém, onde se procederam as análises. Após a constatação do parasitismo pelo gênero Henneguya, foi realizada a observação em microscópio óptico e em microscópio de contraste de interferência diferencial (DIC). Foi realizada técnica histológica de impregnação em parafina e coloração dos cortes histológicos em Ziehl-Neelsen. As análises necroscópicas dos espécimes de H. marginatus revelaram que 80% (40/50) destes apresentavam cistos esbranquiçados na porção óssea dos filamentos branquiais, repletos de esporos do gênero Henneguya. O presente estudo revelou infiltrado inflamatório nas imediações dos cistos. Além disso, a técnica especial de coloração em Ziehl-Neelsen possibilitou marcar os cistos de Henneguya sp. no tecido ósseo e de esporos isolados na estrutura de tecido branquial. As descrições desses achados histopatológicos mostram que esse parasita é muito invasivo e produz danos aos seus tecidos do hospedeiro.

Recurrent primary hydatid disease of tibia / Quiste hidatídico primario recurrente de la tibia

Hydatid disease is caused by a cestode, Echinococcus. Its intermediate hosts are herbivores but humans can be accidental hosts. Hydatid disease is endemic in some parts of America, Australia, the Mediterranean region, Central Asia, and Central and Eastern Europe. The organs most frequently affected by Echinococcus are the liver and the lungs. Primary involvement of the skeleton is rare. Cases have been reported in the vertebrae, pelvis, humerus and femur. The location of hydatid cysts in the tibia is rarely described in the medical literature. We, herein, report a case of primary hydatid cyst of the tibia presenting with a pathologic fracture simulating benign bone cystic lesion. The diagnosis of hydatid bone disease was not suspected preoperatively. This case emphasizes the importance of considering hydatid disease in the differential diagnosis of cystic bone lesions, especially in individuals coming from regions where the disease is endemic.

La enfermedad hidatídica es causada por un céstodo del género equinococo. Sus huéspedes intermediarios son animales herbívoros, pero los seres humanos pueden ser huéspedes accidentales. La enfermedad hidatídica es endémica en algunas partes de América, Australia, la región mediterránea, Asia central, y Europa central y oriental. Los órganos más frecuentemente afectados por los equinococos son el hígado y los pulmones. Es raro un compromiso primario del esqueleto es rara. Se han reportado casos en las vértebras, pelvis, húmero y fémur. La localización de los quistes hidatídicos en la tibia, raramente se describe en la literatura médica. Aquí reportamos un caso de quiste hidatídico primario de la tibia acompañado de fractura patológica simulando una lesión quística benigna del hueso. El diagnóstico de la enfermedad hidatídica ósea no fue sospechado preoperatoriamente. Este caso destaca la importancia de considerar la hidatidosis en el diagnóstico diferencial de las lesiones óseas quísticas, especialmente en individuos procedentes de regiones donde la enfermedad es endémica.

Hidatidosis ósea: Reporte de 5 casos y revisión de la literatura / Bone hydatidosis: Report of five patients

Bone location of hydatid cysts occurs in 0.5 to 3 percent of all cases of hydatidosis. The most common bones involved are spine, long bones and pelvis. We report five patients with bone hydatidosis. A 24-year-old male consulting for paraparesis and numbness of lower limbs; CAT scan showed hydatid cysts located in D2, D3 and D4 vertebral bodies. A 47-year-old male consulting for a fracture of the femur; X rays disclosed multiple hydatid cysts in the femur. A 13-year-old female consulting for claudication of the right lower limb; X ray examination showed a hydatid cyst in the iliac bone. A 21-year-old women presenting with weakness of the lower limbs and voiding problems; CAT scan showed a vertebral hydatidosis and spinal cord compression. A 67-year-old female presenting with weakness of the lower limb and loss of sphincter control; CAT scan showed hydatid cysts in D4 vertebral body.

A varied presentation of hydatid cyst: a report of four cases with review of literature.

Contrary to the popular perception, hydatosis does not remain restricted to certain geographical locales but is a global health concern, particularly in the rural countryside. Although liver and lung involvement account for the majority of the lesions, primary breast, bone, kidney and soft tissue hydatosis with features of cystic echinococcosis are extremely rare. We report four such rare cases of cystic echinococcosis which were diagnosed and treated in our institute.

[Primary hydatidosis of the chest wall. Report of 5 cases]

Hydatidosis is an endemic affection in Tunisia. Bone echinococcosis is a relatively rare entity accounting for only 0.5-2% of all hydatid cysts in humans and chest wall is an uncommon site for the disease. Report of a new case. We report about this talk 5 cases concerning 2 men and 3 women [mean of age 35,4 years] explored for parietal mass [4 cases], or chest pain [1 case]. Diagnosis was suspected on radiologic findings in all cases. All patients underwent surgery. Medical treatment was associated in 2 cases. Histopathology of resected specimen confirmed diagnosis of echinococcosis. No recurrence was observed during follow-up period

Hydatidosis of tibia

A 71 year old female from a village was admitted to Ahwaz Imam Khomeini hospital in southwestern part 7 years ago with pain and swelling at the upper and middle third of right tibia. Operation was performed and permanent sinus secretion of the midportion occurred. After 7 years the patient returned to hospital recently with warmness, swelling and edema and sinus secretion in middle and upper of right leg. Radiological examination revealed osteolytic metaphyseal and diaplyseal lesion with honey combs appearance transitional zone was narrow without reactive bone and incomplete fractures were also noted. Abdominal sonography was normal. Wound discharge culture indicated Klebsiella, Proteus and E. coli. After antibiotic therapy the patient was put under general anesthesia and complete excision of middle part of tibia from proximal metaphysic to distal metaphysis was performed. During the operation laminated layers and daughter cysts of hydatid cysts were seen. The lesion was washed with 0.5% of NO3Ag and 3cm under the tibia plateau to 7cm of tibia planoid was debrided and cement spacer was used for filling the spaces. Histopathologic examination of the resected specimen confirmed the hydatid cyst. The patient was prescribed albendazole and discharged from the hospital. She is being followed up now

case report of hydatid disease in long Bone

Hydatid cyst, caused by echinococcus granulosa, can produce tissue cyst everywhere in body. Skeletal cystic lesion is rare especially in long bones like tibia and because of its unusual presentation, its diagnosis may easily be missed, unless be kept in mind

Hydatid Disease of bones and joints

Hydatid disease commonly affects the liver and lungs. The bones and joints are involved in 1-2% of total hydatid disease cases. Two cases of osseous hydatid disease seen recently are being reported. One male patient who had hydatid disease of the humerus, presented as pathological fracture and another female patient presented with tumour like mass in the sacral area. At operation extensive currettage and lavage was done in both the cases. The humerus was then fixed with K-nail. This patient developed recurrence which was subsequently operated with currettage, lavage, K-nail refixation and bone cement filling. On 5 to 15 months follow-up both patients are doing well without signs of recurrence and the fracture has united well

Hidatidosis maxilofacial / Maxillofacial hydatidosis

Se presenta un caso de hidatidosis ósea con localización Maxilofacial. Se trataría del cuatro caso descripto en la literatura mundial en los últimos 20 años y el primero en Latinoamérica. Se revisa el cuadro clínico, los hallazgos del laboratorio, radiología y estudios de mayor complejidad. Se destaca el valor de la Anatomía Patológica como medio certero de diagnóstico etiológico y como terapéutica de elección la extirpación quirúrgica