ABSTRACT
Background & objectives: Chittoor virus (CHITV) belongs to genus Orthobunyavirus, family Bunyaviridae. It has been isolated from various species of mosquitoes and pig from different parts of India. Five isolates of CHITV were characterized at the molecular level and compared with other Batai viruses (BATV) to find out any kind of reassortment in their genome. Methods: Complete nucelocapsid (S), glycoprotein (M) and partial RNA polymerase (L) segments of CHITV were amplified and sequenced. These sequences were compared with those of Batai viruses, isolated from different geographical locations in Asia, Africa and Europe. Results: Phylogenetic analysis revealed CHITV as a variant of BATV. High level of conservation was seen among the CHITV isolates studied. The CHITV sequences showed clustering in one lineage with the sequences from Japan and Malaysia, however, BATV sequences from Europe and Africa formed a separate phylogenetic lineage. Interpretation & conclusions: The study indicates the presence of a single genotype of CHITV circulating in India, despite the involvement of different hosts in the natural cycle by this virus. Analysis of the sequences of the S, M and L segments of genome indicated that the virus has not undergone any reassortment. This virus has not caused any epidemic involving humans, however, replication of the virus in different mosquito and vertebrate hosts species suggests that it is a cause of concern.
Subject(s)
Animals , Bunyamwera virus/analysis , Bunyamwera virus/isolation & purification , India , Molecular Diagnostic Techniques/methods , SwineABSTRACT
By RT-PCR and TAIL-PCR, the full coding region of Batai virus isolated in China (YN92-4 strain)was sequenced for the first time. According to the results, the genome of the virus contained three segments S, M and L of 947, 4,371 and 6,860 nucleotides, respectively. The S segment coded a nucleoprotein of 234 amino acids and a nonstructural protein of 102 amino acids, the M and L segments coded a precursor protein of 1 ,435 amino acids and RNA polymerase of 2,239 amino acids, respectively. Compared with the full coding sequence of Batai viruses isolated out of China, the S and M segments of YN92-4 and ON-7/B/01 showed the highest homology in nucleotide and amino acid sequenes with similarity of 97.7% (100%) and 95.7% (98%), respectively. Since there was no full coding sequence information on the L segment in GenBank for the reference, the L segment of YN92-4 was compared with that of Bunyamwera virus and the homology of nucleotide and amino acid was 73.5%and 81.6%, respectively. Phylogenetic analysis showed YN92-4 strain was clustered into one group with the prototype of Batai virus (MM2222). The results suggested that the YN92-4 strain had no occurrance of genetic reassortment (like Ngari virus) and was close to the Batai virus (ON-7/B/01 strain) isolated from cattle serum in Japan.
Subject(s)
Animals , Cattle , Amino Acid Sequence , Base Sequence , Bunyamwera virus , Genetics , China , Clinical Laboratory Techniques , Cloning, Molecular , Genome, Viral , Genetics , Hemorrhagic Fever with Renal Syndrome , Molecular Sequence Data , Open Reading Frames , Polymerase Chain Reaction , Reassortant Viruses , Genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNAABSTRACT
<p><b>BACKGROUND</b>To study the molecular characteristics of YN92-4 strain isolated from mosquitoes in Yunnan Province and define its classification.</p><p><b>METHODS</b>The S segment of YN92-4 strain was amplified and sequenced by 2 different sets of primers. The phylogenic tree of S fragment was constructed by Phylip bio-software. The amino acid sequences of N and NSs proteins were also studied.</p><p><b>RESULTS</b>YN92-4 strain could be amplified by 2 sets of primers respectively, S segment showed a highest homology with Batai virus (X73464), reached 96.4%, the homology of protein N and NSs amio-acid sequence with Batai virus was 99.1% and 98% respectively.</p><p><b>CONCLUSION</b>The YN92-4 strain belongs to Batai virus, this is the first report of molecular biological identification of Batai virus in China.</p>
Subject(s)
Animals , Amino Acid Sequence , Bunyamwera virus , Classification , Genetics , China , Culicidae , Virology , DNA, Complementary , Chemistry , Genetics , Molecular Sequence Data , Phylogeny , RNA, Viral , Genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Sequence Homology, Amino AcidABSTRACT
Sete vírus foram isolados de camundongos sentinela, expostos em área de floresta, na Regiäo da Mata Atântica, Estado de Säo Paulo, Brasil, de 1974 a 1981. Estäo incluidas cinco amostras de Maguari, uma Kairi e uma de Tucunduba. Foi a primeira vez que esses vírus foram detectados no Estado de Säo Paulo. Os isolados foram identificados por testes sorológicos de Hemaglutinaçäo, Inibiçäo de Hemaglutinaçäo, Fixaçäo de Complemento e Neutralizaçäo em camundongos lactentes. Os resultados mostram que esses vírus pertencem os sorogrupo Bunyamwera. Como existem relatos de doença humana causada por vírus pertencente a esse grupo, conclue-se que é necessário estudar os aspectos ecológicos envolvendo os ciclos de manutençäo desses vírus, assim como a possibilidade de sua transmissäo em seres humanos no Estado de Säo Paulo, Brasil
Subject(s)
Bunyamwera virus/isolation & purification , Mice/virology , Epidemiological Monitoring , Viruses/isolation & purification , Serologic TestsABSTRACT
Relatam-se os dados clínicos e laboratoriais de um caso de moléstia humana causada por vírus idêntico ou antigenicamente muito relacionado ao arbovírus do Grupo C Caraparu, em um morador da regiäo do Vale do Ribeira, Estado de Säo Paulo, sudeste do Brasil. O fato apresenta interesse médico sanitário pois embora existam evidências da presença de inúmeros arbovírus na área, os únicos casos comprovados de doença por esses agentes foram os de encefalite pelo vírus Rocio durante a epidemia ocorrida em 1975-1977. Os resultados dos testes sorológicos sugerem diferença anti-gênica entre as cepas de vírus Caraparu isoladas nos Estados de Säo Paulo e Pará e proximidade antigênica entre a cepa de Caraparu de Säo Paulo e o vírus Bruconha
Subject(s)
Adult , Humans , Male , Bunyamwera virus/pathogenicity , Bunyaviridae Infections/epidemiology , Brazil , Bunyamwera virus/isolation & purification , Bunyaviridae Infections/diagnosisABSTRACT
En investigaciones de campo realizadas en forma independiente sobre la ecologia de los arbovirus en Arizona, EUA, y Sao Paulo Brasil, se aislaron dos virus del grupo Anopheles A. El virus aislado en Arizona (743-366), obtenido de mosquitos Anopheles freeborni, para el que se propone el nombre de virus Virgin River y el virus aislado en Sao Paulo (H-32580, obtenido de un ser humano) estaban vinculados serologicamente con el complejo Tacaiuma (TCM). Asimismo, se determino que los virus 743-366 y H-32580 son variantes de un subtipo del TCM (SPAr 2317). Sin embargo, es posible distinguir uno del otro. Se describen las circunstancias en que se hicieron estas observaciones y se resalta su significado en la epidemiologia, la genetica y la nomenclatura de la familia virica Bunyaviridae.
Durante pesquisas de campo não relacionadas sobre a ecologia dos arbovírus no Arizona, Estados Unidos, e em São Paulo, no Brasil, isolaram-se dois vírus dos Anopheles, grupo A. 0 vírus isolado no Arizona (743-366 tirado de mosquitos Anopheles freebornz) para o qual se propóe dar o nome de vírus Virgin River e o vírus isolado em São Paulo (H-32580 tirado de um ser humano), estavam serologicamente relacionados no âmbito do complex Tacaiuma (TCM). Determinou-se também que o 743-366 e o H- 32580 são variantes de um subtipo (SPAr2317) do TCM. Contudo, o 743-366 e o H-32580 diferenciam-se um do outro. Este artigo descreve as circunstancias em que se fueram essas observações e a sua significacão epidemiológica, terminológica e genética em relacão com a família dos Bunyaviridae.