ABSTRACT
We investigated the serotypes, distributions, and antimicrobial resistance of Campylobacter jejuni isolates from humans and animals as a source of infection in poultry between 2002 and 2003. A total of 50 C. jejuni isolates from humans and 29 C. jejuni isolates from poultry were studied for serotype using the Penner serotyping scheme and the drug susceptibilities of the isolates which were determined for 7 antimicrobial drugs using the disk diffusion method. Serotype B (10%), serotype E (8%) and serotype R (8%) were found in humans isolates, while serotype A (27%) was most freguently isolated from poultry, followed by serotype K (21%) and serotype C (13%). Resistance in human isolates to cephalothin was high (100%). Resistance to trimethoprim/sulfamethoxazole, ciprofloxacin, and nalidixic acid were observed in 90, 82 and 78% of isolates, respectively. Most of the isolates (88%) were susceptible to erythromycin. High levels of resistance to drugs (ciprofloxacin and nalidixic acid) were observed in the isolates from poultry. These results indicate the importance of poultry as a reservoir of C. jejuni infection in Thailand is limited. In addition, a high proportion of the isolates were resistant to antimicrobial drugs, particularly the quinolone group.
Subject(s)
Animals , Campylobacter Infections/blood , Campylobacter jejuni/drug effects , Disease Susceptibility , Drug Resistance, Bacterial , Humans , Poultry/blood , Poultry Products/microbiology , Quinolones/pharmacology , Thailand/epidemiologyABSTRACT
La proteína C reactiva se ha usado en múltiples enfermedades infecciosas en la orientación de la etiología viral o bacteriana; su utilidad en relación al síndrome diarreico agudo (SDA) se desconoce. Con la hipótesis que las infecciones bacterianas enteroinvasoras muestran un valor mayor de PCR que las virales, se estudiaron 47 niños con SDA. Se realizaron: PCR, 2 coprocultivos, tinción violeta bicarbonato de Hucker y aislamiento de rotavoris por inmunoelectroforesis. Se detectaron 11 casos con bacteria enteroinvasoras en deposiciones, 9 con rotavirus , 1 mixto y 26 pacientes con estudio etiológico negativo. Para las infecciones virales el promedio, mediana y rango de PCR fueron (mg/lt): 13,55; 8; (0-75) y para las bacterias invasoras fueron: 116,7; 116; (1-318) respectivamente (p<0,01). Ocho de 11 niños con infección bacteriana mostraron PCR > 30 mg/lt versus 1 de 9 con infección viral (p=0,0091); 7 de los primeros tuvieron valores > 100 mg/lt. Los resultados obtenidos confirman nuestra hipótesis