ABSTRACT
To observe the effects of heterograft of glomus cells of carotid body on hemiparkinsonian rat models, rats with unilateral 6-hydroxydopamine (6-OHDA)-induced lesions of the right dopaminergic neurons of substantia nigra received intrastriatal glomus cells heterograft. Apomorphine-induced rotation was monitored for 30 min at various time points after grafting. The striata were cut and examined for dopamine content by HPLC and for immunohistochemical staining of tyrosine hydroxylase positive neurons (TH+) at the end of the experiments. The results showed that apomorphine-induced rotational behavior was significantly reduced for 12 weeks and the dopamine contents were significantly elevated after grafting (P < 0.01), and TH+ cells survived better. The present study demonstrates that intrastriatal heterograft of glomus cells within carotid body in rats with 6-OHDA-elicited lesions could reduce apomorphine-induced rotational behavior and elevate the dopamine contents and numbers of TH+ cell surviving within striatum, and can serve as a new and effective alternative for Parkinson disease.
Subject(s)
Carotid Body/cytology , Carotid Body/transplantation , Cell Transplantation , Dopamine/metabolism , Neurons/metabolism , Parkinson Disease/metabolism , Parkinson Disease/surgery , Random Allocation , Rats, Sprague-Dawley , Stereotaxic Techniques , Transplantation, HeterologousABSTRACT
Los quimiorreceptores son estructuras específicas ampliamente distribuidas en el organismo. El cuerpo carotídeo es el representante principal de este sistema, por lo que es el mejor estudiado y sirve de punto de comparación para el conocimiento de otros quimiorreceptores. El quimiorreceptor ciliar ha sido identificado únicamente en aves, pero su estructura no ha sido descrita en detalle. Este trabajo representa una breve comunicación preliminar.