ABSTRACT
Influence of both dietary and bath administration of Eucalyptus globules labill was evaluated on some immunological variables of common carp [Cyprinus carpio] under temperature less than optimum in order to determine stimulatory effect of the essential oils. Fish weighing 30-35 g were bathed or fed with different doses of 30, 60 and 120 micro/L or mg/kg feed for a period of 8 days. Serum lysozyme activity, bactericidal activity, total white blood cells, total protein, globulin and albumin were measured on days 1, 2, 8, 15 and 23 after the essential oils administration. On day 23 post administration the remaining fish from each group were intraperitonealy injected with killed Aeromonas hydrophila [6x10[8] cells/ml] and antibody titer was measured 3 weeks later. The obtained results showed that Eucalyptus globules had a limited immunostimulatory effect on these immunological variables although antibody titers and total white blood cells in some test groups were significantly [p<0.05] higher than the control one. The reduction of the immunological factors is probably related to the lower water temperature, inappropriate administrating dose and duration of essential oils administration
Subject(s)
Animals , Carps/immunology , Oils, VolatileABSTRACT
The present experiment has been designed to find out the effect of heavy metals on the immunological parameters of Labeo rohita and Catla catla as these are major pollutants of the fish habitat. The effect was observed on mean W B C count, lymphocyte and neutrophil count and other related parameters like general behaviour and stress on the controlled and experimentally exposed fishes to sub-lethal concentration of cadmium. Observations were-decreased in mean W B C and percent lymphocyte count, whereas neutrophilia was observed in both the species when exposed to sublethal concentrations of cadmium. The agglutination test conducted on the fishes exposed to cadmium revealed that the titre were higher in the exposed fishes indicating that the fishes are under stress. The study is relevant in the light that the pollutants causes sufficient stress to fish and ultimately affects its performance to harvest he benefits in the form of growths etc