Evaluation of cellular toxicityfor cisplatin, arsenic and a-cetaminophen in the cancer and normal cell line

Cell culture is a process in which the cells ware isolated from original tissue, dispersed in liquid media and then placed in culture plate where the cells adhere together and propagate. Today, this method is used for assessment of cell toxicity, its mechanisms and effect of different compounds on intracellular components. Clonogenic assay was used for assessment of cell toxicity and amount of cell death after a specific time during which cells were exposed to different compounds. Thus, IC50 in caner cell lines [HePG2, SKOV3 and A549] and normal cell [LLCPK1, CHO and HGF1] was assessed after exposure to cisplatin, acetaminophen and arsenic. Results showed that acetaminophen has maximum resistance and minimum sensitivity in CHO line with IC50 = 16.7 +/- 1.06 HePG2 with IC50 = 18.6 +/- 1.29. On the other hand, cisplatin showed minimum resistance and maximum sensitivity in HePG2 with IC50 = 0.87 +/- 0.07 and HGF1 with IC50 = 1.6 +/- 0.21 and lastly, arsenic showed minimum resistance and maximum sensitivity in A549 with IC50 = 4.59 +/- 0.29 and LLCPK1 with IC50-1 +/- 0.37. According to the evaluated IC50, there were differences between results of sensitivity of cell lines exposed to the three drugs [P < 0.05]. Entirely, resistance in cancer cell lines was lower than normal cells. The results showed the importance of cell defensive mechanisms encountering different substances like glutathione

Secretion from neuropeptide-treated splenocytes modifies ovarian steroidogenesis

There are evidences for modulation of immune function by the sympathetic nervous system and its principal neurotransmitter norepinephrine (NE) throgugh superior ovarian nerve (SON)-coeliac ganglionnoradrenergic postganglionic innervation of the spleen. Seven days after SON transection at 53 days of age, the rat splenocytes were isolated and then cultured for 48h. These culture media, used to simulate ovaries from 60-day- old intact rats (neither SON-transected nor sham-operated) at diestrus 2 stage, in in vitro incubations, showed adecrease in progesterone release, an increase in estradiol release and no change in androstenedione release in relation to splenocyte culture media from control (sham-operated) rats.When esplenocytes from SON transected (SON-t) rats were treated with vasoactive intestinal peptide (VIP) or neuropeptide Y (NPY), both at 16-6M for 24h, their secretions increased the progesterone release while decreasing the estradiol release from the intact ovaries, compared with the secretions of untreated splenocytes from SON-t rats. Although the secretions of splenocytes treated with VIP decrease the androstenedione release from de ovaries, the treatment with NPY produced no change in hormone release. In the present paper the ovarian steroidogenic response, which was modified by the effects of an in vivo SON transection on spleen cells, was reverted by an in vitro system in which the splenocytes were treated with VIP or NPY. This could indicate that the spleen of SON-t rats does not receive those neuropeptides by neural route however, when they are added to splenocyte culture in vitro, the cell secretions revert the profile of steroid hormones released from the intact ovary. We also present functional evidence for modulation of the immune function by sympathetic nervous system and neurotransmitters other than NE.

Sensibilidad celular al colágeno vascular en pacientes con trombogene trombo-angeítis obliterante (enfermedad de Leo Buerger) / Celular sensibility to vascular collagen in patients with thrombogen thromboangiitis obliterant (Leo Buerger's disease)

La Tromboangeitis Obliterante (TAO) es una panarteritis segmentaria en vasos de mediano y pequeño calibre que afecta fundamentalmente varones, fumadores, menores de 40 años. Su etiología es desconocida, pero se cree que participan mecanismos de autoinmunidad. Se estudiaron 7 pacientes (6 varones y 1 hembra) con TAO de menos de 1 año de evolución. 3 con vasculitis y 10 controles sanos. Se exploró la proliferación de las células mononucleares en presencia de fitohemaglutinina (PHA) y colágeno tipo I y III. Los resultados sugieren: a) Proliferación normal en presencia de PHA tanto en los pacientes con TAO como en aquellos con vasculitis; b) Sólo 2 pacientes con TAO y ninguno con vasculitis mostraron sensibilidad celular al colágeno. Podemos concluir, que la sensibilidad celular al colágeno pudo ponerse de manifiesto en TAO de corto tiempo de evolución