Effectiveness of a pre-procedural mouthwash in reducing bacteria in dental aerosols: randomized clinical trial

Abstract The aim of this randomized, single blinded clinical trial was to evaluate the effect of a pre-procedural mouthwash containing cetylpyridinium chloride (CPC), zinc lactate (Zn) and sodium fluoride (F) in the reduction of viable bacteria in oral aerosol after a dental prophylaxis with ultrasonic scaler. Sixty systemically healthy volunteers receiving dental prophylaxis were randomly assigned to one of the following experimental groups (15 per group): (i) rinsing with 0.075% CPC, 0.28% Zn and 0.05% F (CPC+Zn+F), (ii) water or (iii) 0.12% chlorhexidine digluconate (CHX), and (iv) no rinsing. Viable bacteria were collected from different locations in the dental office on enriched TSA plates and anaerobically incubated for 72 hours. The colonies were counted and species were then identified by Checkerboard DNA–DNA Hybridization. The total number of colony-forming units (CFUs) detected in the aerosols from volunteers who rinsed with CPC+Zn+F or CHX was statistically significantly (p<0.05) lower than of those subjects who did not rinse or who rinsed with water. When all locations were considered together, the aerosols from the CPC+Zn+F and CHX groups showed, respectively, 70% and 77% fewer CFUs than those from the No Rinsing group and 61% and 70% than those from the Water group. The mean proportions of bacterial species from the orange complex were statistically significantly (p<0.05) lower in aerosols from the CPC+Zn+F and CHX groups compared with the others two groups. In conclusion, the mouthwash containing CPC+Zn+F, is effective in reducing viable bacteria in oral aerosol after a dental prophylaxis with ultrasonic scaler.

Efficacy of two mouthwashes with cetylpyridinium chloride: a controlled randomized clinical trial

Abstract This study aimed to evaluate the anti-plaque and anti-gingivitis effects of two mouthwashes containing cetylpyridinium chloride (CPC), in comparison to negative control mouthwash. One hundred and twenty subjects were randomly assigned to study groups: test (0.075% CPC and 0.28% zinc lactate), positive control (0.07% CPC) and negative control mouthwash without CPC. All volunteers were examined by a calibrated examiner for the Quigley-Hein Plaque Index (Turesky modification) and Löe-Silness Gingival Index (GI). Gingival severity was also measured by the percentage of sites with positive gingival bleeding. During six weeks, oral hygiene consisted of brushing twice daily with a toothbrush and toothpaste and rising with their assigned mouthwash. Plaque and gingival parameters were assessed at baseline, after four and six weeks of product use. Statistical analyses were performed separately for plaque and gingival indices, by ANOVA, paired t-test and ANCOVA (α < 0.05). After 4 and 6 weeks, all mouthwashes groups presented statistically significant reductions in plaque and gingival parameters as compared to baseline. In comparison to the positive control, the test group presented additional reductions in dental plaque of 19.8% and 16.8%, after 4 and 6 weeks, respectively. For GI, the additional reductions in the test group were 9.7% and 14.3%, at 4 and 6 weeks, respectively. The test group showed additional reduction of 35.3% and 54.5% in the gingival severity, at week 4 and 6, respectively. It is concluded that the mouthwash containing CPC and zinc lactate presents significant anti-plaque and anti-gingivitis effects as compared to positive and negative control mouthwashes.

Eficacia del uso concomitante de dentífrico con Triclosán a 0.3 por ciento y copolímero PVM/MA a 2.0 por ciento, enjuague bucal a base de cloruro de cetilpiridinio a 0.05 por ciento y terapia de raspado/alisado radicular en pacientes con gingivitis / Efficacy of concomitant use of dentifrice with 0.3 per cent Triclosan and 2.0 per cent PVM/MA copolymer, 0.05 per cent cetylpyridinium chloride mouthrinse and scaling/root planning therapy in patients with gingivitis

Introducción: La placa dentobacteriana es el principal factor etiológico en el desarrollo y progresión de la gingivitis. Comúnmente se han sugerido productos de cuidado oral como cremas dentales y/o enjuagues bucales como adyuvante en la terapia mecánica. Objetivo: Evaluar la eficacia del uso combinado de un dentífrico a base de Triclosán a 0.3 por ciento y copolímero PVM/MA a 2.0 por ciento (Colgate Total 12®) y enjuague bucal a base de cloruro de cetilpiridinio a 0.05 por ciento (Colgate Plax®) en pacientes con gingivitis marginal crónica después de una terapia de raspado y alisado radicular comparado con un grupo control. Material y métodos: Se realizó un ensayo clínico controlado, aleatorizado y doble ciego en 50 pacientes sanos con diagnóstico sistemático de gingivitis marginal crónica. Se dividieron en grupo A experimental y grupo B control. Se evaluó índice gingival de Lõe y Silness e índice de placa de Quigley Hein modifi cado por Turesky al inicio y al fi nal del estudio; el periodo de estudio fue de seis semanas. Al inicio se realizó a cada paciente tratamiento de raspado y alisado radicular y se le instruyó sobre el uso correcto de los productos. Al concluir la sexta semana se tomaron mediciones finales bajo las mismas condiciones que las iniciales...

Introduction:Dental plaque is considered to be the main etiological factor in the development and progression of gingivitis. Oral care products such as toothpaste and mouthwash have commonly been rec-ommended as an adjunct to mechanical periodontal therapy. Objective:To evaluate the effi cacy of using a dentifrice containing 0.3% triclosan and 2.0% PVM/MA copolymer (Colgate Total 12®) in combination with a 0.05% cetylpyridinium chloride mouthwash (Colgate Plax®) in patients with chronic marginal gingivitis following scaling and root-planing therapy compared to a control group. Material and methods:A randomized double-blind controlled clinical study was performed on 50 systemically healthy patients diagnosed with chronic marginal gingivitis. These were divided into two groups: A (experimental) and B (control). The Lõe and Silness gingival index and the Turesky modifi ca-tion of the Quigley-Hein plaque index were recorded at baseline and after 6 weeks (the duration of the study). Patients were treated with scaling and root-planning, and all received instructions on the proper use of the oral care products. Final measurements were taken at the end of the six-week assessment under the same conditions as those at the time of the baseline assessment...

Effect of cetylpyridinium chloride with xylitol on the formation of biofilm anddevelopment of gingivitis

Aim: To assess the effect of the combination of cetylpyridinium chloride and xylitol on the formation of dental biofilm and development of experimental gingivitis. Methods: A crossover, double-blind, placebo-controlled study was conducted and divided into two phases of 21 days each with a time interval of 10 days between them. A modified experimental gingivitis model was used and 31 volunteers were randomly divided into 2 groups. The volunteers performed daily mouthwashes twice a day with the test solution containing cetylpyridinium combined with xylitol or a placebo solution. On day 0 and day 21 of each phase the Plaque Index (PI) and Gingival Index (GI) of each volunteer were measured. During this phase, the volunteers brushed their teeth with standard toothbrushes and dentifrice, protecting the third quadrant with a toothshield. After brushing, the toothshield was removed and the mouthwash was used. Results: The PI values observed in the Test Group at baseline and on day 21 were 0 (0.00 – 0.03) and 0 (0.22-0.48) respectively, and in Control Group 0 (0.00 – 0.03) and 1 (0.45 – 0.81) (inter-group analysis - McNemar test, p<0.05). For GI, the values obtained in the Test Group were 0 (0.00 – 0.03) and 1 (0.48 – 0.71), at baseline and day 21 and in Control Group 0 (0.00 – 0.03) and 1 (0.58 – 0.84) (inter-group analysis - McNemar test, p>0.05). Conclusions: The test solution had a positive effect on dental biofilm control. However, it was not capable of preventing the development of experimental gingivitis.

Comparative efficacy of two daily use mouthrinses: randomized clinical trial using an experimental gingivitis model

Two antimicrobial agents, a fixed combination of essential oils (EOs) and 0.07 percent cetylpyridinium chloride (CPC) are found in commercially available mouthrinses, Listerine® Antiseptic and Crest® Pro HealthTM, respectively. Both mouthrinses have been shown to control dental plaque and gingivitis in short and longer term studies. The aim of this study was to determine the comparative effectiveness of these two mouthrinses using a 2-week experimental gingivitis model. Qualified subjects were randomly assigned to one of three mouthrinse groups: a fixed combination of EOs, 0.07 percent CPC, or negative control (C) rinse. Following baseline clinical assessments and a dental prophylaxis, subjects began a two-week period in which they rinsed twice daily with their assigned rinse and abstained from any mechanical oral hygiene procedures or other oral care products. Subjects were reassessed at the end of the two-week period. One hundred and forty-seven subjects were randomized and 142 completed this study. After two weeks use, the EOs rinse was superior (p < 0.011) to the CPC rinse in inhibiting the development of gingivitis, plaque, and bleeding, with 9.4 percent and 6.6 percent reductions compared to CPC for gingivitis and plaque, respectively. Both rinses were superior to the negative control rinse (p < 0.001). This study demonstrates that the essential oil-containing mouthrinse has superior antiplaque/antigingivitis effectiveness compared to the 0.07 percent CPC-containing mouthrinse without mechanical oral hygiene influence.

Self-performed supragingival biofilm control: qualitative analysis, scientific basis and oral-health implications

Patients frequently fail to achieve an optimal mechanical plaque control. However, many patients are not confident about using mouthrinses, and many professionals refuse to prescribe them for regular daily use. Aiming at achieving a better understanding of the use and prescription of mouthrinses in periodontics, 24 dentists with different dental educational levels were purposively chosen and interviewed in a qualitative research. Partial data was presented at the 15th Congress of the Brazilian Association for Oral Health Promotion (ABOPREV), and full data was presented at the 88th General Session and Exhibition of the International Association for Dental Research (IADR). The professionals' answers were confirmed or rejected through scientific data analysis. Additionally, scientifically supported answers were provided for the interviewees' most frequent unanswered questions. Around half of the participants (46 percent) reported that they recommended the use of mouthrinses, although a high percentage (64 percent) of the dentists answered that they knew very little about the efficacy of mouthrinses and also about the oral benefits (54 percent) provided by them. All interviewees reported that they were aware of the fact that their patients, and themselves, failed to floss and, less frequently, to brush their teeth, and all of them believed that oral health impacts overall systemic health. Seventy five percent answered that using mouthrinses was safe. Most participants (55 percent) did not declare themselves as mouthrinse users. We concluded that dentists with different levels of dental education have only partial knowledge related to mouthrinse use in periodontics. The use of effective mouthrinses on a daily basis is justified and can help patients achieve or maintain a healthier mouth. A healthier mouth will positively impact patients' quality of life and could also benefit their overall systemic health.

Comparative antiplaque and antigingivitis efficacy of three antiseptic mouthrinses: a two week randomized clinical trial

The objective of this randomized, examiner blind, parallel group, controlled clinical trial was to compare the antiplaque and antigingivitis efficacy of an essential oil-containing mouthrinse (EO) to two mouthrinses containing 0.05 percent Cetylpyridinium Chloride (CPC), one with alcohol and one alcohol-free, using a two-week experimental gingivitis validated-model with a 5 percent hydroalcohol rinse serving as the negative control. One hundred and fifty-nine subjects, 56 males and 103 females; ranging in age from 18 to 58 years in good general health were assigned to one of the four treatment groups: EO (n = 40), 0.05 percent CPC with alcohol (CPCa, n = 39), 0.05 percent CPC alcohol-free (CPCna, n = 40), and 5 percent hydroalcohol negative control (n = 40). The Mean Turesky Modification of the Quigley-Hein Plaque Index (PI) and the Mean Modified Gingival Index (MGI) were the primary efficacy endpoints and were evaluated at baseline and at two weeks. Following baseline examinations, subjects received a complete dental prophylaxis and began supervised rinsing with their assigned mouthrinse twice daily for two weeks, as their sole oral hygiene measure; 151 subjects completed the trial. Two weeks after baseline the EO adjusted mean PI and MGI scores were significantly lower than those of both CPC rinses and negative control (p < 0.001). In conclusion, the EO mouthrinse demonstrated significantly greater antiplaque and antigingivitis efficacy than both CPC-containing mouthrinses and the negative control.

O uso de enxaguatórios bucais no controle da halitose / Use of the mouthrinses on halitosis control

A halitose é causada principalmente pela presença dos compostos sulfurados voláteis produzidos por bactérias proteolíticas na cavidade bucal por meio da degradação dos substratos orgânicos presentes na saliva, no fluido do sulco gengival, nos tecidos moles e duros da boca. Diferentes produtos, como os enxaguatórios bucais, vêm sendo utilizados em ampla escala pela população para o controle da halitose, como um suplemento à limpeza mecânica dos dentes e do dorso da língua. O objetivo da presente revisão de literatura é avaliar a eficácia de alguns enxaguatórios bucais disponíveis no mercado nacional na redução dos compostos sulfurados voláteis e, consequentemente, da halitose.

Oral malodor is mainly caused by the presence of volatile sulfur-compounds produced by proteolitic bacteria in the oral cavity by means of degradation of organic substrates presents in saliva, crevicular fluid, oral soft and hard tissues. Various products, as mouthrinses, have been used in large scale by the population to control halitosis as a supplement to mechanical cleaning of teeth and tongue dorsum. The aim of this review is to evaluate the efficacy of some mouthrinses available in the national market in the reduction the volatile sulfur compounds and, consequently, the halitosis.

Anticaries potential of a fluoride mouthrinse evaluated in vitro by validated protocols

This blind and randomized study tested in vitro, using validated protocols, the anticaries potential of an experimental fluoride mouthrinse. One-hundred enamel slabs, half sound and half with caries-like lesions (carious), all with known surface microhardness (SMH), were submitted to 3 treatment groups: A) a placebo mouthrinse (negative control); B) a positive control mouthrinse containing 0.05 percent NaF; and C) an experimental formulation containing 0.05 percent NaF and cetylpyridinium chloride as an antibacterial substance. To evaluate the formation of F products on enamel, sound (n=10) and carious (n=10) slabs were treated with the formulations during 10 min and loosely and firmly-bound F formed in enamel were determined after extraction with alkali and acid, respectively. To evaluate the inhibition of enamel demineralization, sound enamel slabs (n=10) were treated with the mouthrinse formulations 2x/day during 1 min and subjected to a pH-cycling regimen simulating a cariogenic challenge (demineralization). To evaluate enamel remineralization, the carious slabs (n=10) were submitted to the treatments 3x/day and subjected to a pH-cycling model simulating a remineralizing condition. After 8 days, enamel SMH was determined again and the percentage of SMH loss or SMH recovery was calculated for the sound and carious slabs, respectively. The experimental formulation was superior to the negative control (p<0.05) and equivalent to the positive control (p>0.05) in the formation of F products in enamel, and in the inhibition of enamel demineralization and enhancement of remineralization. These data suggest that the tested experimental fluoride mouthrinse has anticaries potential.

Este estudo in vitro, cego e randomizado avaliou o potencial anticárie de uma formulação experimental de enxaguatório fluoretado (F), usando protocolos previamente validados. Blocos de esmalte (120), 60 hígidos e 60 com lesão artificial de cárie, com microdureza de superfície (MDS) conhecida, foram submetidos a 3 grupos de tratamentos: A) Enxaguatório placebo (controle negativo); B) Controle positivo contendo NaF 0,05 por cento e C) Uma formulação experimental contendo NaF 0,05 por cento e cloreto de cloreto de cetil-piridínio como antibacteriano. Para testar a capacidade de formação de produtos fluoretados no esmalte, blocos hígidos (n=10) e cariados (n=10) foram tratados durante 10 min e os fluoretos fracamente e fortemente ligados formados foram determinados após extração com álcali e ácido, respectivamente. Para avaliar a inibição da desmineralização do esmalte, os blocos hígidos (n=10) foram tratados com as formulações 2 x/dia durante 1 min e submetidos a um modelo de ciclagem de pH, simulando o processo de desmineralização. Para avaliar a ativação da remineralização do esmalte, os blocos com lesões de cárie (n=10) foram tratados 3 x/dia e submetidos a um modelo de ciclagens de pH, simulando uma condição de remineralização. Após 8 dias, a MDS do esmalte foi novamente determinada e as porcentagens de sua redução ou aumento foram calculadas, respectivamente para os blocos hígidos ou com lesão de cárie. A formulação experimental foi superior ao controle negativo (p<0,05) e equivalente ao positivo (p>0,05), na formação de produtos fluoretados no esmalte e na inibição de sua desmineralização e ativação da remineralização. Conclui-se que o enxaguatório experimental apresenta fluoreto ativo com potencial para controlar cárie dental.

Atividade antimicrobiana de produtos para controle de placa: verificaçäo da influência de fatores como concentraçäo, forma de apresentaçäo e tempo de contato na atividade antimicrobiana de produtos químicos sobre Streptococcus mutans e Lactobacillus: estudo "in vitro" / Antimicrobial activity of the products used for plaque control: influence of concentration, means of presentation and contact period, in antimicrobial activity from chemical substances over Streptococcus mutans and Lactobacillus

O presente estudo teve por objetivo verificar "in vitro" a influência de fatores como concentraçäo, forma de apresentaçäo e tempo de contato na atividade antimicrobiana de produtos químicos sobre Streptococcus mutans e Lactobacillus. Foram testados o Triclosan na forma dentifrício (Signal G) e soluçäo (Gengi-Dent) e o Cloreto de Cetilpiridínio na forma soluçäo (Cepacol), tendo os produtos sofrido várias diluiçöes. No teste de difusäo do Agar, todos os produtos foram ineficazes para as cepas de Lactobacillus acidophilus e Lactobacillus casei, porém todos eles proporcionaram inibiçäo de crescimento sobre os Streptococcus mutans tipos "d" e "f", sendo que o Triclosan soluçäo apresentou os maiores alos de inibiçäo. Evidenciou-se ainda neste teste que ao sofrer diluiçäo, o produto mostra queda do poder inibitório. Através do teste de poder germicida, constatou-se a ineficácia dos produtos sobre cepas de Lactobacillus acidophilus e os fatores concentraçäo e tempo de contato só interferiram sobre o Triclosan dentifrício e o Cloreto de Cetilpiridínio. Quando a forma de apresentaçäo do Triclosan foi soluçäo, esses fatores näo tiveram influência. Observou-se nos dois testes que as cepas de Lactobacillus säo mais resistentes aos produtos quando comparados ao Streptococcus mutans. Conclui-se o trabalho considerando que os fatores como concentraçäo, forma de apresentaçäo e tempo de contato iräo influenciar a capacidade germicida, de acordo com a resistência dos microorganismos aos produtos químicos

Estudo em pacientes portadores de aparelho ortodôntico, correlacionando os índices de placa e gengival, à escovaçäo dental, e com bochechos de fluoreto de sódio, e com Cepacol / Plaque and gingival index in orthodontic patients undergoing toothbrushing and mouthrinses with sodium fluoride and Cepacol

O trabalho foi realizado, utilizando uma amostra de pacientes do Curso de Pós-Graduaçäo em Ortodontia do Instituto Metodista de Ensino Superior, brasileiros, leucodermas de ambos os sexos na faixa etária de 10 anos a 22 anos e 8 meses, com idade média de 14 anos e 11 meses, com dentiçäo permanente, portadores de maloclusäo de Classe I e Classe II de Angle. A amostra avaliada somou 60 pacientes, sendo estes orientados e supervisionados para executar a técnica oblíqua de escovaçäo. Esta foi dividida em três grupos de 20 pacientes. O 1§ grupo realizou apenas escovaçäo ; o 2§ grupo escovaçäo associada a bochechos diários com fluoreto de sódio a 0,05 por cento e o 3§, escovaçäo mais bochechos diários com soluçäo de Cepacol 1:4.000. Durante a pesquisa foram realizadas cinco avaliaçöes, sendo a primeira, uma semana após a colocaçäo de aparelho ortodôntico fixo; a segunda, seis meses após a primeira mediçäo; a terceira, um ano após a primeira mediçäo; a quarta, um mês antes da remoçäo do aparelho ortodôntico e a quinta, um mês após a remoçäo total do aparelho. Essas avaliaçöes tinham a finalidade de verificar as alteraçöes ocorridas no índice de placa, segundo Silness & Loe e no índice gengival, segundo os mesmos, ambos por nós modificados, durante o transcorrer do experimento. De acordo com a análise dos resultados obtidos pudemos concluir que : 1 - Há uma correlaçäo positiva entre índice de placa e índice gengival no grupo escovaçäo. 2 - O grupo que utilizou a escovaçäo associada a bochechos com Cepacol apresentou resultados mais estáveis ao longo do experimento. 3 - O grupo que usou bochechos com flúor em relaçäo ao índice de placa e índice gengival näo apresentou vantagem, pois näo houve qualquer diferença durante o estudo. 4 - A escovaçäo associada a bochechos de Cepacol parece ser uma conduta de higiene promissora em relaçäo ao controle do índice de placa em pacientes portadores de aparelho ortodôntico fixo

Influência de bochechos com cepacol sobre a placa dentária. Parte I - Avaliação da quantidade de placa formada / Influence of the moutwashes with \"Cepacol\" on the dental plaque

Este estudo demonstrou que bochechos com solução antisséptica contendo cloreto de cetilpiridínio, realizados por adultos jovens, 3 vezes ao dia, proporcionou uma redução de 21,78//nos escores altos de placa e de 31,04//do peso úmido de placa em comparação aos bochechos realizados com uma solução contendo os mesmos ingredientes com exceção do cloreto de cetilpiridínio (placebo). Estes resultados permitem recomendar bochechos com solução antisséptica contendo cloreto de cetilpiridínio, como coadjuvante dos procedimentos mecânicos empregados no controle da placa dentária

Estudo em pacientes portadores de aparelho ortodôntico, correlacionando os índices de placa e gengival, à escovaçäo dental, e com bochechos de fluoreto de sódio, e com cloreto de cetilpiridíneo / The study about plaque and gingival index, in patients who use orthodontics appliances, in relationship of toothbrushing, sodium fluoride rinse and with cetylpiridinium solution

O trabalho foi realizado, utilizando uma amostra de pacientes do curso de Pós-Graduaçäo em Ortodontia do Instituto Metodista de Ensino Superior, brasileiros, leucodermas, de ambos os sexos na faixa etária de 10 anos a 22 anos e 8 meses, com idade média de 14 anos e 11 meses, com dentiçäo permanente, portadores de maloclusäo de Classe I e Classe II de Angle. A amostra avaliada somou 60 pacientes, sendo estes orientados e supervisionados para executar a técnica oblíqua de escovaçäo. Esta foi dividida em três grupos de 20 pacientes. O 1§ grupo realizou apenas a escovaçäo; o 2§ grupo, escovaçäo associada a bochechos diários com fluoreto de sódio a 0,05 por cento e o 3§, escovaçäo mais bochechos diários com soluçäo de Cepacol 1:4.000. Durante a pesquisa foram realizadas cinco avaliaçöes, sendo a primeira, uma semana após a colocaçäo do aparelho ortodôntico fixo; a segunda, seis meses após a primeira mediçäo; a terceira, um ano após a primeira mediçäo; a quarta, um mês antes da remoçäo do aparelho ortodôntico e a quinta, um mês após a remoçäo total do aparelho. Essas avaliaçöes tinham a finalidade de verificar as alteraçöes ocorridas no índice de placa, segundo Silness & Loe e no índice gengival, segundo Loe & Silness, ambos por nós modificado, durante o transcorrer do experimento. De acordo com a análise dos resultados obtidos pudemos concluir que: 1 - Há uma correlaçäo positiva entre índice de placa e índice gengival no grupo escovaçäo. 2 - O grupo que utilizou a escovaçäo associada a bochechos com Cepacol apresentou resultados mais estáveis ao longo do experimento. 3 - O grupo que usou bochechos com flúor em relaçäo ao índice de placa e índice gengival näo apresentou vantagens, pois näo houve qualquer diferença durante o estudo. 4 - A escovaçäo associada a bochechos de Cepacol parece ser uma conduta de higiene promissora em relaçäo ao controle do índice de placa em pacientes portadores de aparelho ortodôntico fixo

Avaliaçäo da presença de hemoculturas positivas a microorganismos anaeróbicos - após o uso como colutório, de soluçäo aquosa de cloreto de cetilpiridínio - decorrentes da remoçäo do fio de sutura aposto na mucosa gengival, após exodontia / Evaluation of the occurrence of positive hemocultures for anaerobic microorganisms (after the use of an aquous solution of cetylpyridinium chloride), in relation to suture removel from the gingiva after exodontia

O presente trabalho visou a constataçäo da ocorrência de hemoculturas positivas, pós remoçäo do fio de sutura, aposto na mucosa gengival depois de exodontia de molar superior, tendo sido feito, previamente, o uso, como colutório, de uma soluçäo aquosa de cloreto de cetilpiridínio 1:4.000 que embebia a regiäo durante um (1) minuto. Foram utilizados seres humanos do sexo masculino, normo-reativos, os quais foram divididos em dois grupos: A e B. O grupo A visava a detecçäo de possíveis bacteriemias transitórias, pela simples punçäo venosa do antebraço e a permanência do "butterfly" pelo tempo de duraçäo do teste (cinco minutos). O grupo B se constituia de pacientes que foram submetidos à avulsäo de dentes molares superiores nos quais, após oito dias, removia-se o fio de sutura. Do grupo A colhia-se 3 ml de sangue imediatamente à punçäo venosa e também um minuto e cinco minutos após. Do grupo B colhia-se a mesma quantidade de sangue, pré-remoçäo do fio de sutura e também um minuto e cinco minutos após a remoçäo do mesmo. Todas as hemoculturas foram processadas em meio de cultura para microorganismos anaeróbios, conforme as normas preconizadas pelo Virginia Polytechnic Institute (VPI). Constatou-se que, em todos os casos do grupo A, (dez indivíduos) as hemoculturas foram negativas. Nos pacientes do grupo B (dezoito pacientes), todas as hemoculturas feitas pré-remoçäo do fio de sutura foram negativas e nas hemoculturas realizadas a um minuto e a cinco minutos, após a remoçäo do fio, houve positivância em 50 por cento dos casos. As colônias de microorganismos encontrados foram identificadas quanto ao gênero