Comparison between solid phase ELISA, dot ELISA and agar gel precipitation test for detection of bursal disease viral antigen in bursal homogenates

Forty-four bursal samples were collected from naturally infected and normal broiler chicks. The samples were homogenized and the homogenates were tested by agar gel precipitation test [AGPT], solid- phase ELISA [by indirect double antibodies s and wich and single antibody ELISA systems] and dot-ELISA for detection of infectious bursal disease virus [IBDv] antigen. The reacted positive samples showed 86.3, 88.6 and 88.6 percentage in AGPT, solid-phase ELISA and dot-ELISA, respectively. The findings suggested that the Dot-ELISA is faster, more economic and easily applicable procedure than the other two techniques. The indirect double antibody s and wich technique is more sensitive than the indirect single antibody ELISA system