ABSTRACT
Two hundred fresh full term placentae of normal pregnancies were collected from the department of Obstetrics and Gynaecology, RIMS, Ranchi, Jharkhand, India to observe the incidence of vascular pattern of chorionic blood vessels of placenta and to note the relationship between vascular pattern of placenta and the birth weight of neonate. Dye was injected into umbilical vessels under normal physiological pressure and diameter of chorionic blood vessels were taken at the centre and periphery. Two types of vascular pattern of chorionic blood vessels of placentae were observed- Dispersal and Magistral. Dispersal pattern was found in 63 percent cases and magistral pattern was observed in 37 percent cases. An interesting observation was noted that the birth weight of neonate was higher in magistral pattern in comparison to dispersal pattern in both sexes...
Doscientas placentas de término de gravidez normales frescas fueron recogidas del departamento de Obstetricia y Ginecología, RIMS, Ranchi, Jharkhand, India. Se observó la incidencia del patrón vascular de los vasos sanguíneos coriónicos de la placenta y la relación entre el patrón vascular de ésta y el peso al nacer del recién nacido. Se inyectó tinta dentro de los vasos umbilicales bajo presión fisiológica normal, y se tomaron los diámetros de los vasos sanguíneos coriónicos en el centro y la periferia. Dos tipos de patrón vascular coriónicos se observaron en la placenta, disperso y magistral. El patrón disperso se encontró en el 63 por ciento de los casos y el patrón magistral se observó en 37 por ciento de los casos. Una observación interesante mostró que el peso al nacer de los recién nacidos fue mayor en el patrón magistral en comparación con el patrón disperso en ambos sexos...
Subject(s)
Humans , Male , Female , Infant, Newborn , Birth Weight , Chorion/blood supply , Placenta/blood supply , Blood Vessels/anatomy & histologyABSTRACT
Angiostatin is a potent angiogenesis inhibitor that is composed of the first four kringles of plasminogen fragment. Angiostatin with one less kringle molecule (kringle 1 to 3) was recently demonstrated to be an effective angiogenic inhibitor. To determine whether recombinant plasminogen kringle 1-3 (rPK1-3) can inhibit the corneal neovascularization induced by potent angiogenic factors; angiogenin, bFGF, or VEGF, hydron polymer discs each containing 2.0 microg of angiogenin, 500 ng of bFGF, or 500 ng of VEGF respectively were implanted into the corneal stroma of 138 rabbit eyes, and then discs each containing 10 microg, 12.5 microg, 20 microg or 30 microg of rPK1-3 were implanted randomly. Discs containing phosphate buffered saline were also implanted as a control. The angiogenesis score on number and length of newly formed vessels on the each of the rabbit's cornea were recorded daily by two observers (blinded). The treated corneas were also examined histologically. Recombinant PK1-3 treated corneas showed less neovascularization induced by all angiogenic factors (p < 0.05). and the extent of inhibition of neovascularization was proportional to the concentration of rPK1-3 (p < 0.05). Histologic examination showed leukocyte infiltration into the corneal stroma on the PBS treated eyes whereas rPK1-3 treated eyes showed only traces of leukocytes. These results of the effective rPK1-3 inhibition of corneal neovascularization induced by angiogenin, bFGF, or VEGF suggest that this angiostatin related fragment, rPK1-3, may be useful in the treatment of various neovascular diseases. Copyright 2000 Academic Press.
Subject(s)
Chick Embryo , Rabbits , Angiogenesis Inhibitors/pharmacology , Angiogenesis Inhibitors/genetics , Animals , Chorion/drug effects , Chorion/blood supply , Cornea/pathology , Cornea/drug effects , Cornea/blood supply , Endothelial Growth Factors/pharmacology , Fibroblast Growth Factor 2/pharmacology , Kringles/genetics , Lymphokines/pharmacology , Microscopy/methods , Neovascularization, Pathologic/drug therapy , Plasminogen/pharmacology , Plasminogen/genetics , Recombinant Proteins/pharmacology , Recombinant Proteins/genetics , Ribonuclease, Pancreatic/pharmacologyABSTRACT
Según metodología descripta en trabajos anteriores, se estudia la contractilidad "in vitro" de los vasos coriales de 30 placentas humanas normales, y el efecto provocado por el aumento del PO**2 en el medio perfusor a expensas de concentraciones de H**2 O**2 de 1.25, 2.5 y 5.0 mM. El H**2 O**2 provoca una respuesta contráctil monofásica; la intensidad de la contraccíon aumenta proporcionalmente a la concentración de H**2 O**2 (F = 8.9 p > 0,01). La indometacina y la aspirina a concentraciones de 2.5 micronM y 0.25 mM respectivamente, inhiben aquella respuesta