Immunoglobulin and C3-activator profile of the microbiology laboratory worker.

The levels of immunoglobulin, C3-Activator and C3c in the sera of members of staff who have been continuously engaged for upwards of 10 years in the microbiological routine laboratory of a teachings hospital were determined. These were compared with the local normals, with those of the junior members of staff in the same laboratory but who had put in less than 5 years continuous service and with a control group. The means in all five determinations were much higher in the senior members of staff than the normal standards of the area, particularly the IgG, which was almost double the local normals. The levels for the junior staff were on the whole slightly higher than the control group. The increase in the levels of immunoglobulins with corresponding increases in C3-Activator and 3Cc suggest that immunological reactions, with the binding of complement in the alternate and classical pathways of activation are taking place in most of these senior workers. In the absence of any manifest illness in the senior staff, the findings may suggest a high degree of immunological protection.

Study of complement C3 and C4 and phagocytic function of the neutrophils before and after splenectomy

The present study showed significant increases in the total leucocytic and neutrophilic counts and in the calculated absolute number of stimulated NBT positive cells after splenectomy in all studied groups [P <0.05]. By contrast, the serum complement C3c and C4 showed insignificant increases after splenectomy in all trial groups [P >0.05]. Nevertheless, the age was not important factor affecting the changes that have been observed in the present study. It can be concluded that the spleen does not share in the synthesis, consumption or catabolism of C3c and C4. Meanwhile, the phagocytic function of neutrophils will increase after removal of the spleen. This might be a part of the whole increase in the number of while cells and neutrophils or due to post-splenectomy infection or the increase in the function of macrophages mediated by the macrophageactivating factors secreted by T-cells since splenectomy induces and increase in T helper and decrease in T suppressor cells

Effect of diuresis on complement C3 and immunoglobulin concentrations in serum and ascitic fluid of patients with post-hepatitic cirrhosis

Serum and ascitic fluid samples were taken from twenty nine patients with post-viral hepatitis liver cirrhosis and ascites to estimate the complement C3 concentrations before and after two-week diuresis in comparison to other ten matching normal control subjects. Before treatment, there was a significant decrease in both serum and ascitic complement C3 concentrations in patients with cirrhotic ascites [P <0.05] when compared to controls. Furthermore, after cirrhotic patients received the diuretic therapy, there was significant increase in ascitic fluid complement C3 [P <0.01] but insignificant change in serum C3 [P >0.05]. This study demonstrated that diuresis of patients with cirrhotic ascites increases the concentrations of ascitic fluid C3 component of the complement and hence may protect the patients from spontaneous bacterial peritonitis

Influence of piroxicam and piroxicam-cyclodextrin inclusions on gastric mucosal ulceration: an experimental study

The study was performed to prove the ulcerogenic effect of piroxicam [as an example of NSAIDs] in stomachs of experimental animals and to evaluate the protective effect of inclusion complexation of piroxicam with beta-cyclodextrin [B-CyD] against gastric irritation. 15 Wistar rats were used in this study. The drugs were given orally for 4 consecutive days and the animals were sacrificed on the fifth day. Their stomachs were examined both macroscopically [by measurement of ulcer index, UI] and microscopically. The study documented the ulcerogenic effect of piroxicam both macroscopically and microscopically. Moreover, it proved the benefit of piroxicam beta- CyD complexation in reducing its gastric ulceration

Immunohistochemical detection of IgG, IgA, IgM and C3 complement in thyroid lesions

The present work focused on the detection of immunoglobulins IgG, IgA, IgM and C3 complement in a wide variety of thyroid diseases as well as in normal thyroid tissue to evaluate the immunologic nature of these lesions. The study was encountered on 111 thyroid specimens selected from the biopsies received at the Pathology Laboratory over a period of 5 years. Formalin fixed paraffin embedded sections were prepared and stained by H and E and immunoperoxidase technique. IgG, IgA and IgM were detected in varying degrees in all types of thyroid lesions and also in normal thyroid tissue. While, positivity for C3 complement was detected in [6/10] cases of toxic nodular goiter, all cases of cretinism with goiter 8, in 11 out of 13 cases of Graves disease, in 14 out of 19 cases of thyroiditis and in 10 out of 16 cases of papillary carcinoma of the thyroid gland. However, no C3 complement could be detected in normal thyroid tissue, in cases of simple goiter, in all cases of thyroid adenoma and in all cases of follicular, anaplastic and medullary carcinoma examined. Results indicated that immunoglobulins are not predictors for diagnosis of autoimmunity in thyroid diseases, but are best considered as indicators for the gland activity. However, C3 and immunoglobulins may be used as useful markers for the diagnosis of papillary carcinoma and its follicular variant from follicular carcinoma

Determinaçäo da fraçäo C3c do complemento por imunodifusäo radial no soro e saliva parotídea de indivíduos clinicamente normais / Determination of C3c fraction complement by radial immunodiffusion in the serum and saliva from the parotid gland of clinically normal subjects

Determinamos a fraçäo do C3c do complemento no soro e na saliva parotídea de 13 indivíduos clinicamente normais pelo método da imunodifusäo radial. O valor médio do C3c sérico foi de 86,92 mg/dl e o intervalo de confiança da média de 73,53 a 100,31 mg/dl. No estudo das salivas das parótidas näo foi detectada concentraçäo mensurável de C3c