ABSTRACT
The present paper evaluates the protocol for the diagnosis of Chagas' diseases and rangeliosis that is suitable for use in remote and scarcely populated rural areas. Beginning with a blood sample taken by venipuncture in the rural dispensary from 350 inhabitants of Casero La Sierra, Cojedes State, Venezuela, samples were analyzed at a laboratory located at a distance of 150 Km. Each blood sample was analyzed for blood and clot culture, artificial xenodiagnosis, inoculation into mice, complement fixation reaction (CFR), and indirect fluorescent antibodies test (IFA). Nine isolates of trypanosomes were obtained and identified as Trypanosoma rangeli, 29.4 percent of the blood samples showed seropositivity for Trypanosoma cruzi, 7.1 percent of total of persons between 6 and 18 years were found infected with T. rangeli, suggesting active transmission of this trypanosome in an endemic area for Chagas' disease. Results indicate that this protocol of study is reliable, economical and sufficiently versatile to study both rangeliosis and Chagas' disease
Subject(s)
Humans , Animals , Mice , Chagas Disease/diagnosis , Trypanosomiasis/diagnosis , Evaluation Study , Fluorescent Antibody Technique, Direct , Rural Population , Complement Fixation Tests/instrumentation , Complement Fixation Tests/methodsABSTRACT
Se estudiaron 58 pacientes en total, siendo 26 mujeres y 32 varones. El promedio de edad fue semejante a los tres grupos (29+/-5,13 anos)La parasitemia fue evaluada mediante xenodiagnosticos seriados (Xd). Cada estudio se realizo con 4 cajas de 10 ninfas del 3er estadio del T. Infestans cada una de acuerdo a la tecnica de Cerisola y Col.> El personal que realizo la lectura en todos los casos ignoraba a que grupo pertenecia el paciente estudiado e igualmente si habia recibido tratamiento o no