ABSTRACT
En esta Parte 4 de la serie de cuatro artículos sobre micetismos se analizan los síndromes que se caracterizan por presentar un período de latencia muy corto, con la aparición de síntomas complejos en menos de 6 horas después de la ingestión de los macromicetos. Se discuten los siguientes micetismos: 1) Toxíndrome muscarínico o colinérgico periférico por especies de Inocybe y Clitocybe. 2) Toxíndrome inmunohemolítico o hemolítico por Paxillus. 3) Toxíndrome neumónico alérgico por Lycoperdon perlatum y por Pholiota nameko. 4) Toxíndrome panterínico o neurotóxico glutaminérgico por compuestos isoxazólicos o síndrome pantherina/muscaria. 5) Toxíndrome coprínico o cardiovascular. 6) Toxíndrome neurotóxico alucinogénico por psilocibina y derivados indólicos. 7) Toxíndrome psicotrópico por estirilpironas y gimnopilinas de Gymnopilus spectabilis o G. junonius. 8) Toxíndrome agudo de rabdomiólisis por Russula subnigricans. 9) Toxíndrome cianogénico por Marasmius oreades. 10) Toxíndrome inmunosupresor por tricotecenos macrocíclicos de Podostroma cornu-damae. 11) Toxíndrome hemolítico debido a ostreolisina de Pleurotus ostreatus y especies relacionadas. Se analizan los síntomas, las toxinas involucradas, los mecanismos de acción, cuando se conocen, y las especies causantes de los micetismos.
This Part 4 of the series of four articles on mushroom poisonings refers to early-onset syndromes, which are characterized by a very short latency period, and the appearance of complex symptoms in less than 6 hours after mushroom ingestion. The following mycetisms are discussed, (1) Peripheral cholinergic, or muscarinic syndrome due to Inocybe and Clitocybe species. (2) Immunohaemolytic or haemolytic syndrome by Paxillus. (3) Allergic pneumonic syndrome due to Lycoperdon perlatum, and Pholiota nameko. (4) Glutaminergic neurotoxic, or pantherinic syndrome by isoxazole compounds or pantherina/muscaria syndrome. (5) Coprinic or cardiovascular syndrome. (6) Hallucinogenic neurotoxic syndrome due to psilocybin and indole derivatives. (7) Psychotropic syndrome by styrylpirones and gymnopilins of Gymnopilus spectabilis or G. junonius. (8) Rhabdomyolysis acute syndrome due to Russula subnigricans. (9) Cyanogenic syndrome by Marasmius oreades. (10) Immunosuppressive syndrome by macrocyclic trichothecenes of Podostroma cornu-damae. (11) Haemolytic syndrome due to ostreolisine of Pleurotus ostreatus, and related species. The symptoms, toxins involved, mechanisms of action, when known, and the species of mushrooms responsible for the mycetisms are analyzed.
Nesta parte 4 da série de quatro artigos sobre intoxicação por cogumelos são analisadas síndromes que se caracterizam por apresentar um período de latência muito breve, com aparecimento de sintomas complexos em menos de 6 horas após a ingestão dos macromicetos. As seguintes intoxicações com cogumelos são discutidas: (1) Toxíndrome muscarínico ou colinérgico periférico por espécies de Inocybe e Clitocybe. (2) Toxíndrome imuno-hemolítica ou hemolítica por Paxillus. (3) Toxíndrome pneumônica alérgica por Lycoperdon perlatum e por Pholiota nameko. (4) Toxíndrome panterínica ou neurotóxica glutaminérgica por compostos isoxazólicos ou síndrome pantherina/muscaria. (5) Toxíndrome coprínica ou cardiovascular (6) Toxíndrome neurotóxico-alucinogênica por psilocibina e derivados indólicos. (7) Toxíndrome psicotrópica por estirilpironas e gimnopilinas de Gymnopilus spectabilis ou G. junonius. (8) Toxíndrome aguda de rabdomiólise por Russula subnigricans. (9) Toxíndrome cianogênica por Marasmius oreades. (10) Toxíndrome imunossupressora por tricotecenos macrocíclicos de Podostroma cornu-damae. (11) Síndrome hemolítica por ostreolisina de Pleurotus ostreatus e espécies relacionadas. São analisados os sintomas, as toxinas envolvidas, os mecanismos de ação, quando conhecidos, e as espécies de cogumelos responsáveis pelas intoxicações.
Subject(s)
Mushroom Poisoning/classification , Mushroom Poisoning/therapy , Trichothecenes , Coprinus , Agaricales , Marasmius , AmanitaABSTRACT
The 1,095 bp gene encoding peroxidase from Coprinus cinereus was synthesized and integrated into the genome of Pichia pastoris with a highly inducible alcohol oxidase. The recombinant CiP (rCiP) fused with the a-mating factor per-pro leader sequence derived from Saccharomyces cerevisiae was secreted into the culture medium and identified as the target protein by mass spectrometry, confirming that a C. cinereus peroxidase (CiP) was successfully expressed in P. pastoris. The endoplasmic reticulum oxidoreductase 1 (Ero1) and protein disulfide isomerase (PDI) were co-expressed with rCiP separately and simultaneously. Compared with the wild type, overexpression of PDI and Erol-PDI increaseed Cip activity in 2.43 and 2.6 fold and their activity reached 316 U/mL and 340 U/mL respectively. The strains co-expressed with Erol-PDI was used to high density fermentation, and their activity reached 3,379 U/mL, which was higher than previously reported of 1,200 U/mL.
Subject(s)
Coprinus , Culture Media , Cytoplasm , Fermentation , Glycoproteins , Metabolism , Mass Spectrometry , Mating Factor , Oxidoreductases Acting on Sulfur Group Donors , Metabolism , Peptides , Peroxidases , Pichia , Metabolism , Protein Disulfide-Isomerases , Metabolism , Protein Folding , Saccharomyces cerevisiae , Saccharomyces cerevisiae Proteins , MetabolismABSTRACT
In order to enhance the utilization efficiency, reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration; we studied the ligninase production by Coprinus comatus, Aspergillus niger and Trichoderma reesei through the plate screening. The results showed that C. comatus mixed culture with T. reesei have a good compatibility and higher yields of Laccase. On the basis of this pre-experiment, we studied the optimal conditions of mixed culture for enzyme production. Under the optimal conditions: the inoculation proportion of C. comatus and T. reesei (5:2), the interval of time (12 h), the temperature 260C, the shake rotation speed 150 r/min, fermented for 3 days, the Laccase activity reached 3267.1 U/mL, increased by 106% contrasted with single culture of C. commatus.
Subject(s)
Coprinus , Metabolism , Culture Techniques , Methods , Fermentation , Oxygenases , Plant Stems , Metabolism , Trichoderma , Metabolism , Zea mays , MetabolismABSTRACT
The principal objective of this study was to acquire basic data regarding the mycelial growth characteristics for the artificial cultivation of Coprinus comatus. 12 URP primers were employed to evaluate the genetic relationships of C. comatus, and the results were divided into three groups. Among six kinds of mushroom media, MYP medium was selected as the most favorable culture medium for C. comatus. The optimal temperature and pH ranges for the mycelial growth of C. comatus were 23~26degrees C and pH 6~8, respectively. The carbon and nitrogen sources for optimal mycelial growth were sucrose and tryptone, respectively.
Subject(s)
Agaricales , Carbon , Coprinus , Cultural Characteristics , Hydrogen-Ion Concentration , Nitrogen , SucroseABSTRACT
Development of oidia, a type of thallic spores from monokaryotic mycelium of Coprinus cinereus was examined with electron microscope and light microscopes. Oidia formation in this fungus is unique in its mode of formation compared with other types of asexual sporogenesis. Oidiogenesis in C. cinereus is carried out in three steps: 1) Formation of oidiophore from the parent mycelium, 2) Formation of initials of oidial cells from swollen oidiophore, 3) Segmentation and detachment of mature oidial cell. Oidiophores appear to spring out singly as a swollen hyphal branches from the normal foot hyphae or sometimes coiled hypha. From the oidiophore, oidial branches sprout out forming a group of 2~6, most often 4 oidial cells and each oidial cell undergoes a single mitosis resulting in 2 oidia. One of the sibling oidial cells in a group is frequently transformed into a new oidiophore, thus oidiogenic structures are tandemly produced at the several different levels.
Subject(s)
Humans , Coprinus , Foot , Fungi , Hyphae , Mitosis , Mycelium , Parents , Siblings , SporesABSTRACT
Degradation of yard wastes by Coprinus truncorum growing in a vertical aereated bioreactor or in flasks was studied. There was a constant decay of reducing sugars in the medium that avoided their accumulation and their possible repression of degradative enzymes. Endoxylanase activity at first showed a similar pattern in both culture conditions, with maximal activity on the 12th day, but flasks maintained a high activity thereafter. Flasks also showed a higher endoglucanase activity with a peak on the 18th day, whereas the maximal value in the bioreactor was reached on the 26th day. No Mn-peroxidase and only low values of laccase activity were found. The measurements of pH and soluble proteins during the incubation period were suitable indicators of the degradation process by C. truncorum.
Subject(s)
Bioreactors , Coprinus , Refuse Disposal/methods , Mycology , Plants , Biodegradation, Environmental , Carbohydrates , Cellulase , Cellulose , Coprinus , Garbage , Hydrogen-Ion Concentration , Lignin , Oxidation-Reduction , Oxidoreductases , Peroxidases , Fungal Proteins/metabolism , Solubility , Temperature , XylosidasesABSTRACT
This study is aimed to investigate about the fungal flora of some agaric fungi in Assiut, Egypt wild mushroom were collected during cold weather from November to February in the years 1995 and 1996 respectively. Twenty three species and one variety belong to 12 genera in 7 families of the order Agaricales from Assuit were identifled. The common genera were Agaricus, Macrolepiota and Corprinus. From the preceeding genera, four species from each genus were collected. Agaricus bitorquis, A. campestris, A. hortensis, A. perraus, Macrolepioya, M. rhacodes, M. rhacodes var. Hortensis, Marcrolepiota sp., Coprinus afamentarus, C. comatus, C. picaceus and C. nosoustii.
Subject(s)
Humans , Agar , Agaricales , Agaricus , Coprinus , Egypt , Fungi , WeatherABSTRACT
Se citan 18 Agaricales sensu lato tóxicos que han sido recolectados en la X región de Chile. Se entrega una descripción de sus características macro y microscópicas más inportantes y se comentan sus aspectos toxicológicos, taxonómicos y ecológicos
Subject(s)
Humans , Agaricales/classification , Plants, Toxic , Agaricus muscarius , Hallucinogens/toxicity , Amanita , Coprinus/pathogenicity , Fungi/pathogenicity , Gastrointestinal Diseases/etiology , Mental Disorders/etiologyABSTRACT
En este trabajo se determinan los hongos superiores (Coprinaceae), que habitan en el Campus de la Universidad Federal de Pernambuco (UFPE), Recife, PE., Brasil. Se estudiaron nueve especies comprendidas en las subfamilias coprinoideae y panaeoloideae, con los géneros coprinus y panaeolus respectivamente. Coprinus está representado por las especies c. auricomus, c. brunneo-fibrillosus, c.fimetarius, c. jamaicensis, c. lagopus, c. niveus, c. plicatilis y c. subimpatiens. Del género panaeolus sólo fue identificada una especie: panaeolus antillarium. C.auricomus, c. brunneo-fibrillosus, c. jamaicensis y c., subimpatiens, son aparentemente las primeras citas para Brasil