ABSTRACT
We constructed an expression cassette of the organophosphorus pesticide degrading (opd) gene under the control of the E8 promoter. Then opd was transformed into tomato fruit using an agroinfiltration transient expression system. beta-Glucuronidase (GUS) staining, reverse transcription-polymerase chain reaction (RT-PCR), wavelength scanning, and fluorescent reaction were performed to examine the expression of the opd gene and the hydrolysis activity on coumaphos of organophosphorus hydrolase (OPH) in tomato fruit. The results show that the agroinfiltrated tomato fruit-expressed OPH had the maximum hydrolysis activity of about 11.59 U/mg total soluble protein. These results will allow us to focus on breeding transgenic plants that could not only enhance the degrading capability of fruit and but also hold no negative effects on pest control when spraying organophosphorus pesticides onto the seedlings in fields.
Subject(s)
Aryldialkylphosphatase , Genetics , Physiology , Coumaphos , Pharmacology , Insecticides , Pharmacology , Solanum lycopersicum , Genetics , Metabolism , Plants, Genetically Modified , Promoter Regions, GeneticABSTRACT
The efficacy of Flumethrin pour on and Coumaphos 50% WP was tested against different stages of Hyalomma dromedarii. With contact method, LC[50] values for larvae and adults were 0.04, 0.03 ug/cm[2] and 0.05, 1.06 ug/cm[2] respectively. The LC[50] for coumaphos against the larvae, adults and eggs using dipping method were 44, 63, and 62 ppm respectively. Flumethrin caused significant reduction in oviposition and hatchability in female ticks that survived sublethal concentrations, while sublethal doses of coumaphos had no effect on the reproductive potential of the tick. The results showed that flumethrin was 8 times more toxic than coumaphos