ABSTRACT
An organic extract from a marine crustacean D. avarus was examined for antiangiogenic activity by using the chick embryo chorioallantoic membrane (CAM) assay. The methanol extract (HCM) inhibited angiogenesis in a dose dependent manner. The extract was further fractionated by bioactivity-guided separation to purify the active fractions successively. This resulted in three fractions HCM1, HCM2 and HCM3. The 50% inhibition shown by HCM was 600 ng/disc, HCM1 was 100 ng/disc and of HCM3 was 2.7ng/disc. HCM3 which was separated by column chromatography and showed single spot on TLC was analysed by GLC and showed the presence of saturated and unsaturated fatty acids such as lauric, myristic, palmitic, stearic, oleic, linoleic. The antiangiogenic activity of the fatty acids obtained from a marine crustacean is reported for the first time.
Subject(s)
Angiogenesis Inhibitors/isolation & purification , Animals , Chromatography, Gas , Crustacea/chemistry , Fatty Acids/isolation & purificationABSTRACT
In polytypic Idotea chelipes species, the slow esterase constitutes a subspecific biochemical marker which discriminates, whithout ambiguity, each of three subspecies. Amylase 2 is a diagnostic locus which serapates I. chelipes bocqueti from the two other subspecies where each is characterized by particular frequencies of the most frequent allele. The analysed electropohoretic zymogram and the intersubspecific cross-breeding results attribute a monomeric structure for this enzyme which depends on diallelic gene