Ultrastructural cell surface alterations in developing embryos of frog (Microhyla ornata) treated with cytochalasins.

Scanning electron microscopic examination of M. ornata embryos treated with cytochalasins A, B and H (CA, CB and CH) showed extensive cell disaggregation resulting in large intercellular spaces and apparent loss of intercellular communication. All the three cytochalasins significantly reduced surface features, such as, filopodia and membrane ruffling which are considered essential for normal morphogenetic movements. Appreciable qualitative differences could not be detected in effects exerted by CA, CB and CH although potency of the three drugs clearly differed. The results demonstrate that in spite of the differences in their primary mechanisms of action, treatment with all the three cytochalasins culminates in comparable effects on the cell surface architecture resulting into abnormal morphogenesis.