ABSTRACT
The present study was carried out to examine the effects of exposure to Pyridostigmine bromide [PB] alone or in combination with Permethrin [Per] and/or N, N-diethyl-m-toluamide [DEET] at three different doses in production of oxidative stress in male Sprague-Dawely rats. Animals were treated with a daily dermal dose of Permethrin and/or DEET for 60 days and/or PB [gavage] during the last 15 days. Hepatic lipid peroxidation, total Superoxide dismutase activity [SOD], Glutathione peroxidase activity and Glutathione [GSH] were analyzed as a biomarker for oxidative stress. The obtained results revealed marked elevations in the level of lipid peroxide product [MDA], SOD, GSHPx and a significant decrease in glutathione content. This study concluded that, the exposure to PB alone or in combination with Permethrin and/or DEET may result in induction of free radicals generation causing lipid peroxidation and disturbance in the antioxidant scavenging system. The hazardous effect of exposure to the three tested compounds combination [PB, Permethrin and DEET] proved to be much more serious than that of PB when administered alone