ABSTRACT
OBJECTIVE@#To test and estimate the forensic application of Goldeneye™ DNA ID 25A Kit.@*METHODS@#The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework.@*RESULTS@#The peak height balance of the different loci was ≥ 42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA.@*CONCLUSION@#Goldeneye™ DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.
Subject(s)
Female , Humans , Asian People/genetics , China , Chromosomes, Human, Y , DNA/genetics , DNA Fingerprinting/standards , Databases, Nucleic Acid , Forensic Genetics/methods , Genotype , Polymerase Chain Reaction , Polymorphism, Genetic , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
OBJECTIVE@#To perform the validation and analysis of forensic parameters of Goldeneye DNA ID 26Y system.@*METHODS@#Based on the validation rules of Scientific Working Group on DNA Analysis Methods (SWGDAM), the kit was assessed from several parts, as test of PCR system, reproducibility, accuracy, and sensitivity, etc. And Y-STR loci of 517 unrelated healthy individuals from Eastern China were genotypes by this kit. The distribution and frequency of haplotype were calculated and forensic parameters of the kit were assessed.@*RESULTS@#The complete profiles can be obtained even when the PCR reaction volume with 6.25 microL. And correct profile was obtained with DNA down to 125 pg. No reproducible peaks were detected with the DNA of common animals and microorganism with the kit. For the male-male mixture testing, average 70% of the minor alleles were obtained when the ratios of 1:19 and 19:1. For the male-female mixture testing, results showed that the sensitivity of the kit was no compromised with the addition of female samples.@*CONCLUSION@#The validation studies demonstrated that Goldeneye DNA ID 26Y system has good sensitivity and specificity, and suitable for mixture testing. The polymorphism of 26 Y-STR loci included in this kit are good for forensic application.
Subject(s)
Animals , Female , Humans , Male , Alleles , Asian People/genetics , China , Chromosomes, Human, Y , DNA , DNA Fingerprinting/standards , Forensic Genetics/methods , Genotype , Polymerase Chain Reaction , Polymorphism, Genetic , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
La "Comisión de Acreditación y Vigilancia de los Laboratorios que practican las pruebas de paternidad o maternidad con marcadores genéticos de ADN", creada por la Ley 721 de 2001, constituyó una Sub-comisión para revisar las normas colombianas sobre los Estándares Básicos Requeridos en los Laboratorios de Pruebas de Paternidad, para que específicamente recomendara los aspectos técnicos pertinentes en Colombia, tomando como referente permanente la Norma ISO 17025. En este documento se consignan estas recomendaciones para Colombia.
Subject(s)
Humans , DNA Fingerprinting/standards , Laboratories/standards , Paternity , ColombiaABSTRACT
Fingerprinting is thought to establish the identify of an individual in forensic cases. The technique is extensively used for forensic purposes. Deoxyribonucleic acid (DNA) is the vehicle of generational transference of heritable unit. While arching markers for genetic disease professor Alec Jeffreys discovered that certain regions of DNA showed variations in the number of tandem repeats known as variable number of tandem repeats (VNTRs). Thus DNA fingerprint was named by observing the number of repeated sequences which differ from individual to individual. The structure of DNA is quite flexible, within the nucleus of each cell resides an identical copy of the individual's genetic material, DNA. The coding regions of the genomic DNA are known as genes. The DNA fingerprinting in forensic science has generated considerable excitement in the criminal justice community. DNA fingerprinting can be applied to identify an individual in criminal and civil cases. Polymerase chain reaction has revolutionised molecular biology it has an ability to amplify (usually fewer than 3000 bp) a particular sequence of DNA into million of copies in a very short period. Consequently only a very tiny amount of an organism's DNA needs to be available originally. This property of polymerase chain reaction has enabled to analyse many forensic samples particularly which are degraded. Microsatellite DNA or commonly as short tandem repeats are scattered throughout the human genome and occur on an average of every 10,000 nucleotides. Microsatellite markers are considered to be the most powerful genetic markers. Collection, preservation and handling are the integral part of DNA fingerprinting analysis. There are various methods to isolate DNA from different biological materials but presently most of the laboratories prefer using FTA paper. The age of humans can be estimated by using DNA based on telomere shortening.