ABSTRACT
In vitro cultivation of trematodes would assist studies on the basic biology of the parasites and their hosts. This is the first study to use the yolk of unfertilized chicken eggs as a simple and successful method of ovocultivation and the first time to obtain the adult-stage of the trematode Cymatocarpus solearis Braun, 1899 (Digenea: Brachycoeliidae). Chicken eggs were inoculated with metacercariae from the muscle of the spiny lobster, Panulirus argus (Latreille, 1804). The metacercariae were excysted and incubated for 576 hr (24 days) at 38degrees C to obtain the adult stage. Eggs in utero were normal in shape and light brown color. The metacercariae developed into mature parasites that have been identified as the adult-stage found in marine turtles. The adult lobsters collected in Quintana Roo State, Mexico, showed the prevalence of 49.4% and the mean intensity of 26.0 per host (n = 87). A statistical study was performed to determine that no parasitic preference was detected for male versus female parasitized lobsters. Morphometric measurements of the adult-stage of C. solearis obtained in our study have been deposited in the National Helminths Collection of the Institute of Biology of the National Autonomous University of Mexico. This study is significant because it is the first time that a digenean of the family Brachycoeliidae has been demonstrated to develop in vitro from metacercariae into adults capable of producing eggs using the yolk of unfertilized chicken eggs. Secondly, this technique allows to obtain the adult stage of C. solearis without the presence of its marine turtle host, allows us to describe the mature parasites, and thus contribute to our understanding of the biology of C. solearis.
Subject(s)
Animals , Culture Media , Egg Yolk/parasitology , Parasitology/methods , Temperature , Trematoda/growth & developmentABSTRACT
O objetivo do presente trabalho foi verificar a presença da Salmonella Enteritidis na gema de ovos que apresentavam nomáximo cinco dias de comercialização e características de qualidade compatíveis com as estabelecidas pela legislaçãobrasileira. Realizou-se a pesquisa de Salmonella Enteritidis em gemas de 96 ovos divididos em grupos de 12 ovos, ou seja,subdivididos em oito lotes. Os resultados foram negativos, não sendo detectados estirpes de Salmonella Enteritidis emnenhum dos lotes estudados. Conclui-se, portanto, ser baixa a ocorrência desta bactéria na gema dos ovos em cascaanalisados, cujos resultados foram compatíveis com os padrões de identidade e qualidade estabelecidos pela legislaçãobrasileira.
The objective was verify the presence of Salmonella Enteritidis in the yolk of eggs that presented no more than five days ofpacking and compatible quality characteristics with the established ones for the Brazilian legislation. We took place the researchof Salmonella Enteritidis in yolks of 96 eggs divided in groups of 12 eggs, in other words, 8 lots. The results, in its totality, werenegative, not detecting Salmonella Enteritidis in none of the studied lots. The absence of Salmonella Enteritidis observedindicates to be low the occurrence of this bacteria in the yolk of the fresh eggs with compatible identity and quality patterns withthe established by the Brazilian legislation.