ABSTRACT
Introducción. Los teratomas son neoplasias que surgen a partir de células germinales pluripotenciales y derivan de dos o más capas de células. Se clasifican en tumores maduros, que contienen tejidos bien diferenciados, o inmaduros, que contienen estructuras inmaduras y embrionarias. Su localización más frecuente son las gónadas; la ubicación mesentérica es infrecuente y se han descrito aproximadamente 40 casos en la literatura mundial. Dentro del abordaje diagnóstico y terapéutico, se emplea la tomografía computarizada y la resonancia magnética nuclear para caracterizar la lesión, evaluar la extensión intraabdominal y la relación con otras estructuras. El diagnóstico debe confirmarse mediante el examen histopatológico. Caso clínico. Paciente femenina de 56 años, con antecedente de carcinoma ductal infiltrante de mama izquierda en remisión, en estudios de seguimiento con hallazgo incidental en tomografía de abdomen de lesión abdominopélvica dependiente del mesenterio, contornos lisos y nivel grasa-líquido. Estudios de extensión con marcadores tumorales negativos. Resultados. Por la alta sospecha clínica e imagenológica de teratoma, fue llevada a resección quirúrgica de la lesión. El examen histopatológico confirmó el diagnóstico de teratoma quístico maduro del mesenterio. Conclusión. El teratoma mesentérico es una entidad clínica rara, que debe ser considerado como uno de los diagnósticos diferenciales de una masa abdominal con efecto compresivo. El diagnóstico se basa principalmente en el examen clínico y los hallazgos imagenológicos. La escisión quirúrgica temprana es el pilar del tratamiento; el abordaje laparoscópico o abierto depende de las características clínicas y la experiencia del cirujano.
Background. Teratomas are neoplasms that arise from pluripotent germ cells, derived from two or more layers of germ cells. They are classified as mature tumors (cystic or solid), which contain well-differentiated tissues, or as immature tumors, which contain immature and embryonic structures. Its most frequent location is the female and male gonads; the mesenteric location is rare and approximately 40 cases have been described in the world literature. Within the diagnostic and therapeutic approach, computed tomography and magnetic resonance imaging are used to characterize the lesion, assess intra-abdominal extension and the relationship with other structures. The diagnosis must be confirmed by histopathological examination. Clinical case. A 56-year-old female patient with a history of infiltrating ductal carcinoma of the left breast in remission. In follow-up studies, incidental abdominal tomography finding of an abdominopelvic lesion dependent on the mesentery at the level of the mesogastrium, smooth contours with fat-liquid level. Extension studies with negative tumor markers. Results. Due to high clinical and imaging suspicion of teratoma, the patient was taken to resection of the lesion. Histopathological examination confirmed the diagnosis of mature cystic teratoma of the mesentery. Conclusion. Mesenteric teratoma is a rare clinical entity and is considered one of the differential diagnoses of an abdominal mass with a compressive effect. Diagnosis is mainly based on clinical examination and imaging findings. Early surgical excision is the mainstay of treatment; laparoscopic or open approach depends on the clinical characteristics and the experience of the surgeon.
Subject(s)
Humans , Teratoma , Abdominal Neoplasms , Pathology , Embryonic Germ Cells , MesenteryABSTRACT
<p><b>OBJECTIVE</b>To perform a genome-wide alternative polyadenylation (APA) profiling in both mouse female germline stem cells (FGSCs) and embryonic stem cells (ESCs) and explore the role of germline-specific APA in the biological behaviors of FGSCs.</p><p><b>METHODS</b>We used a high-throughput sequencing-based method 3T-Seq to profile the genome-wide 3' termini of the transcripts and delineate all the APA sites in mouse FGSCs and ESCs. The genes with altered APA sites in FGSCs compared with ESCs were analyzed with DAVID Gene Ontology tool for their biological roles.</p><p><b>RESULTS</b>We identified a total of 50243 APA sites in 16973 genes. In FGSCs, 1148 genes were shown to have alterations in 3'UTR length, among which 795 ( 66%) genes had shortened and 353 (34%) had lengthened 3'UTR. Some of the genes with shortened 3'UTR were involved in germ cell development.</p><p><b>CONCLUSIONS</b>Our genome-wide APA profiling analysis reveals a cell type-specific APA alternation in FGSCs, and APA-mediated 3'UTR alteration contributes to germline-related biological process. This study provides a framework for understanding the post-transcriptional regulation mechanisms in FGSCs.</p>
Subject(s)
Animals , Female , Mice , 3' Untranslated Regions , Cell Differentiation , Embryonic Germ Cells , Metabolism , Embryonic Stem Cells , Metabolism , Gene Expression Regulation , Genome , PolyadenylationABSTRACT
Researches on manipulating pluripotent stem cells derived from blastocysts or primordial germ cells (PGCs) have a great advantage for developing innovative technologies in various fields of life science including medicine, pharmaceutics, and biotechnology. Since their first isolation in the mouse embryos(1), stem cells or stem cell-like colonies have been continuously established in the mouse of different strains(21), cattle(2, 3), pig(4, 5), rabbit(6, 7), and human(9). However, full-term development originated from established pluripotent cells, which is an absolute criterion for proving cell pluripotency and differentiation, has only been reported in the mouse(22). Due to technical difficulties, no further progress has been made in the establishment of animal embryonic stem (ES) cell line. Alternatively, the use of embryonic germ (EG) cells was selected to establish an animal stem cell line. EG cells also have pluripotent characteristics, which were proven by morphological assay, intracellular alkaline phosphatase activity, and reactions with cell surface-specific markers. The finding of Labosky et al.(23) on germline chimera development after transfer to embryos clearly proved the pluripotency of EG cells and their similar characteristics with ES cells. Avian transgenesis has an unlimited value in biotechnology industry, since its applicability as a bioreactor has proven to be greater than that of mammalian species(24). In the chicken, EG cells can be extensively utilized instead of ES cells for efficiently inducing transgenesis mediated by germline transmission. Recently, PGCs collected from the embryonic gonad were suggested to be useful in establishing avian stem cells. Technical feasibility and applicability of gonadal PGCs (gPGCs) to germline chimera production were also confirmed(25) and a gPGC culture system to establish EG cells was subsequently developed(15).