ABSTRACT
O presente estudo teve como objetivo avaliar macro e microscopicamente a mucosa gastrintestinal de cães domiciliados, que apresentaram sinais gastrintestinais crônicos, por meio de exame endoscópico e da avaliação histopatológica. Os sinais clínicos, a macro e a microscopia das mucosas gástrica e duodenal foram correlacionados. Foram avaliados 20 cães de diferentes raças, sendo 7 machos e 13 fêmeas, com idade variando entre 1,7 a 15,8 anos, que apresentavam cronicidade dos sinais característicos da Doença Inflamatória Intestinal (DII). O estudo foi prospectivo e transversal, realizado em cães atendidos no Hospital Universitário de Medicina Veterinária da Universidade Federal Fluminense (HUVET - UFF) e em clínicas privadas do município do Rio de Janeiro. Amostras de mucosa gastroduodenal foram obtidas por meio de biópsia endoscópica e foram processadas por técnica histológica de rotina e coradas por Hematoxilina-Eosina e Giemsa. As amostras foram submetidas à análise histopatológica para pesquisa de bactérias espiraladas sugestivas de Helicobacter spp. O teste rápido da urease também foi realizado. Os sinais clínicos, e os achados macro e microscópicos foram confrontados e correlacionados. Neste estudo, foi possível estabelecer correlação positiva entre a perda de peso e a presença de enantema no antro, no corpo e no duodeno além de correlação entre alteração do apetite, a presença de infiltrado inflamatório gástrico e a positividade no teste da urease. Este estudo demonstrou a importância da observação de alguns sinais clínicos como a perda de peso e alterações do apetite no curso da Doença Inflamatória Intestinal. Desta forma, enfatiza-se que cães que apresentem tais sinais clínicos devem ter a Doença Inflamatória Intestinal incluída no grupo de diagnósticos diferenciais evitando, com isso, negligenciar uma enfermidade que, cada vez mais, acomete animais de pequeno porte.(AU)
The aim of this study was to evaluate macro and microscopic gastrointestinal mucosa of domiciled dogs, with chronic gastrointestinal symptoms by endoscopy and histopathological evaluation, correlating clinical signs, macro and microscopy characteristics/aspects/changes of duodenal and gastric mucosa. Twenty dogs of different breeds were evaluated with with chronic characteristic signs of Inflammatory Bowel Disease (IBD), being 7 males and 13 females, with ages ranging from 1.7 to 15.8 years old. The study was prospective and cross-sectional, conducted in dogs treated at the Teaching Veterinary Medicine Hospital of the Universidade Federal Fluminense (HUVET-UFF) and private clinics in the city of Rio de Janeiro. Gastroduodenal mucosal samples were obtained by endoscopic biopsy and were processed by routine histological technique and stained with hematoxylin- eosin and Giemsa. The samples were submitted to histopathological analysis for the detection of spiral bacteria suggestive of Helicobacter spp. The rapid urease test was also performed. The clinical signs, the macro and microscopic findings were compared and correlated. In this study, we established a positive correlation between weight loss and the presence of erythema in the antrum, body and duodenum as well as correlation between change in appetite and the presence of gastric inflammatory infiltrate and positivity in the urease test. This study demonstrated the importance of the observation of some clinical signs such as weight loss and appetite changes in the course of IBD. Thus, it is emphasized that dogs showing these clinical signs should have the IBD included in the differential diagnoses group avoiding thereby neglecting a disease that increasingly affects small animals.(AU)
Subject(s)
Animals , Dogs , Inflammatory Bowel Diseases/diagnosis , Inflammatory Bowel Diseases/pathology , Inflammatory Bowel Diseases/veterinary , Endoscopy, Gastrointestinal/veterinaryABSTRACT
Avaliaram-se os aspectos da mucosa gastroduodenal em cães tratados experimentalmente com nimesulida, monofenilbutazona e meloxicam. Foram formados quatro grupos com oito cães. Os grupos 1, 2 e 3 receberam, respectivamente, tratamento com nimesulida, monofenilbutazona e meloxicam durante 21 dias, e o grupo 4 foi utilizado como controle. Todos os animais foram avaliados por exames endoscópicos do estômago e duodeno antes do experimento e aos 10 e 21 dias de tratamento. Os cães não manifestaram qualquer alteração clínica ou laboratorial durante o período de estudo. A avaliação endoscópica da mucosa gastroduodenal apresentou apenas lesões consideradas de baixo grau. Esses antiinflamatórios mostraram-se seguros para o trato gastrintestinal de cães clinicamente saudáveis
The gastroduodenal mucosa in dogs experimentally treated with nimesulide, monophenylbutazone and meloxicam was evaluated. There were four groups with eight dogs in each. Groups one, two and three were given nimesulide, monophenylbutazone and meloxicam, respectively, during 21 days and group four was used as control. All animals were evaluated by gastroduodenoscopy before the study and on the 10th and 21st days. The dogs did not show any clinical or laboratorial changes during the study. The endoscopic evaluation of gastroduodenal mucosa showed only low degree lesions. These anti-inflammatory drugs showed to be safe for the gastrointestinal tract in healthy dogs
Subject(s)
Animals , Male , Female , Adult , Dogs , Anti-Inflammatory Agents, Non-Steroidal , Dogs/physiology , Endoscopy, Gastrointestinal/veterinary , Gastrointestinal Motility/physiologyABSTRACT
Con el objeto de determinar la frecuencia de presentación de Helicobacter spp. se realizó un estudio en caninos de Valdivia, Chile, a través de biopsia gástrica de las zonas fúndica y pilórica obtenida por endoscopía y analizadas mediante las pruebas de ureasa e histología. Los resultados indican que la bacteria Helicobacter spp. fue detectada en todos los perros. La prueba de ureasa dio resultados positivos a Helicobacter en el 86,2 por ciento de las muestras de la zona fúndica y en el 75,9 por ciento del antro pilórico. El examen histológico reveló la bacteria en todas las muestras fúndicas y en el 92 por ciento de las muestras del antro pilórico. En la zona fúndica las frecuencias obtenidas entre la prueba de ureasa e histología resultaron estadísticamente significativas, a diferencia de lo que se obtuvo en la zona del antro pilórico. La bacteria fue encontrada en perros de todas las edades de ambos sexos. Las muestras que resultaron positivas a la prueba de ureasa, reaccionaron principalmente entre las 3 y 24 horas de incubación. En el examen histológico se determinó el grado de infección por el Helicobacter spp., siendo las muestras clasificadas mayormente en los grados moderado a marcado, ubicándose sobre la superfiscie de la mucosa, en las fosas gástricas, glándulas gástricas y en las células parietales.
A study was carried out in order to determinate Helicobacter spp. in dogs of Valdivia city, Chile. Gastric mucosal biopsy from fundus and pyloric antrum were obtained by endoscopy and analized by urease test and histology.Stomach endoscopic appearance, frecuency presentation of Helicobacter spp. according to sex and age, time reaction to urease test, degree of infection and distribution Helicobacter spp. were analized. Results indicated that Helicobacter spp. was detected in all dogs. The urease test was positive in 86.2% of the fundus samples and in 75.9% of the pyloric antrum samples. Histological examination revealed the bacterium in all of the fundus samples and in 92% of the antrum samples. In fundus samples the frecuency obtained by urease test and histology resulted statistically significant, different to the results obtained in the antrum region. Helicobacter spp. was found in young and old animals of both sexes. Samples that gave positive results in the urease test reacted mainly between 3 to 24 hours post incubated. Histological examination to determinate degree of infection to Helicobacter spp., were classified moderate to marked degree and detected on the mucosal surface, gastric pits, gastric glands and parietal cells.