ABSTRACT
Abstract Increasing biological activity and phytochemical investigations on Eryngium species showed its potential as pharmaceutical approach. Eryngium kotschyi Boiss. is one of the species of Eryngium genus and is endemic to Turkey. It is known that this plant is traditionally used in the South-western part of Turkey for the treatment of various diseases. This study focuses on cytotoxic activities of methanol extract and ethyl acetate, n-butanol and water sub-extracts from E. kotschyi in A549, COLO 205 and MDA-MB-231 cell lines by Sulforhodamin B assay and qualitative and quantitative determination of phytochemical constituents in active extract by LC-MS/MS. From the result of the study, it was seen that E. kotschyi ethyl acetate (EKE) sub-extract showed the strongest cytotoxic effect with the low IC50 values (50.00; 31.96 and 22.26 µg/mL in A549; COLO 205 and MDA-MB-231 cells at 48 h, respectively). Preliminary examination of the mass spectrums revealed the presence of 15 phytochemical compounds in active sub-extract and 7 of them was quantiï¬ed. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (485.603 µg/mgextract), chlorogenic acid (62.355 µg/mgextract) and caffeic acid (59.266 µg/mgextract). Moreover, this preliminary study on inhibitory activity of EKE sub-extract suggests further toxicologic investigations and detailed investigation on cytotoxic effect of various combinations of determined compounds
Subject(s)
Turkey/ethnology , Cells/metabolism , Eryngium/anatomy & histology , Phytochemicals/adverse effects , Pharmaceutical Preparations/administration & dosage , Cell Line/classification , A549 Cells/metabolism , Acetates/administration & dosageABSTRACT
Culantro de monte (Eryngium foetidum L.) is a biennial herb of tropical origin very frequent in America, Africa and the Caribbean. With the arrival of Europeans in the fifteenth century, it managed to be distributed to various locations in Asia and Europe. The present study was carried out in the San Antonio de Padua community in the Province of Los Ríos - Ecuador to collect information, through interviews with the residents, regarding the nomenclature and uses of this aromatic herb. It was found that 52% of the interviewees mention a nutritional use, especially as a condiment for fish soup, buns and stews, while 26% say they use this plant for medicinal purposes in infusions to cure stomach and joint pain. The variety of metabolites that it possesses, such as eryngial (E-2-Dodecenal), extends its range of applications in modern medicine. We concluded that the transfer of ethnobotanical information still remains in the community, but the young generations are unaware of several of this plant's applications.
El culantro de monte (Eryngium foetidum L.) pertenece a la familia Apiaceae, es una hierba terrestre bienal de origen tropical muy frecuente en América, África y el Caribe, con la llegada de los europeos en el siglo XV, logró distribuirse a varias regiones de Asia y Europa. El presente estudio se realizó en la comunidad San Antonio de Padua en la provincia de Los Ríos Ecuador, tuvo como objetivo recopilar información, mediante entrevistas escritas a los pobladores, referente a la nomenclatura y usos de esta hierba. Se encontró que un 52% de los entrevistados le da un uso culinario para preparar sopas de pescado, sancochos, bollos y estofados, mientras que un 26% dice utilizar esta planta con fines medicinales en infusiones para tratar en el ser humano dolores estomacales y dolores de las articulaciones. La variedad de metabolitos que posee, como el eryngial (E-2-Dodecenal), amplía su rango de aplicaciones en la medicina moderna. Se concluye que el flujo de información etnobotánica se mantiene en la comunidad, pero las nuevas generaciones desconocen las varias aplicaciones que tiene esta especie.
Subject(s)
Humans , Ethnobotany , Eryngium/chemistry , Surveys and Questionnaires , Apiaceae , EcuadorABSTRACT
A espécie Bromelia antiacantha Bertol. (Bromeliaceae), conhecida como caraguatá ou gravatá, foi avaliada quanto à sua propriedade hemolítica frente a hemácias de sangue de carneiro. O extrato aquoso dos frutos apresentou atividade hemolítica a partir da diluição de 0,85%, enquanto que o extrato aquoso das folhas apresentou hemólise a partir da diluição de 0,90%. Tal atividade pode estar relacionada à presença de saponinas, uma vez que o estudo químico do extrato metanólico das folhas resultou no isolamento da saponina daucosterol, um fitoesterol glicosilado
The specie Bromelia antiacantha Bertol. (Bromeliaceae), popularly known as caraguatá or gravatá, was evaluated as for its hemolytic property against sheep red blood cells. The aqueous extract of the fruits presented hemolytic activity starting from the dilution of 0,85%, while the aqueous extract of the leaves presented hemolysis starting from the dilution of 0,90%. The activity should be related to the presence of saponins, once the chemical study of the leaves methanolic extract resulted in the isolation of the saponin daucosterol, a glycosylated phytosterol.