Subject(s)
Humans , Male , Child, Preschool , Thrombocytopenia , Purpura, Thrombocytopenic, Idiopathic , Parvovirus B19, Human , ErythrovirusABSTRACT
To estimate the prevalence of IgG antibodies against B19 virus [B19V] in Makkah and Jeddah, Saudi Arabia. B19V-specific IgG antibodies were detected by a commercial indirect enzyme-linked immunosorbent assay in sera of 400 paediatric patients [185 males and 215 females] aged 1-17 years. Results: Of the 400 patients, 80 [20%] had sera positive for B19V-specific IgG. The difference in the prevalence of the antibodies between genders was not statistically significant [p = 0.9]. The prevalence of anti-B19V antibodies increased significantly in the age group of 12-17 years as compared to younger patients [37.5 vs. 18% in those aged 1-11 years; p = 0.006 This study indicated a high prevalence of IgG antibodies against B19V in paediatric patients with an increase in age
Subject(s)
Humans , Male , Female , Immunoglobulin G/blood , Seroepidemiologic Studies , Enzyme-Linked Immunosorbent Assay , Erythrovirus/immunology , Age Distribution , Antibodies, Viral/blood , PrevalenceABSTRACT
O parvovirus humano B19 foi isolado e caracterizado de amostra clínica de um paciente, infectado no Japão, e que apresentou os sintomas de febre e erupção cutânea após sua chegada ao Brasil. A infecção por parvovírus foi confirmada por meio de seguintes ensaios: Elisa para detecção de anticorpos IgM antiparvovirus B19 e técnica de polymerase chain reaction (PCR). Um fragmento da região NS1-VP1 foi diretamente submetido ao seqüenciamento do nucleotídeo. A análise filogenética parcial do B19, frente às várias seqüências disponíveis no GenBank, indicou que PV B19 isolado correspondeu ao genótipo 1.
Human parvovirus B19 was identified and characterized in sample collected from a patient who was infected in Japan, and the symptoms as fever and rash appeared after arriving to Brazil. The occurrence of virus infection was confirmed by both assays: Elisa parvovirus B19-specific IgM antibody detection andpolymerase chain reaction (PCR). A fragment of NS1-VP1 region was directly submitted to nucleotide sequencing. Partial phylogenetic analysis of B19 sequences, including several sequences available in GenBank, indicated that the isolated HPV B19 corresponded to genotype 1.
Subject(s)
Molecular Epidemiology , Erythema Infectiosum , Erythrovirus , Base Sequence , Epidemiological MonitoringABSTRACT
Hydrops fetalis (HF) is a disease characterized by generalized subcutaneous edema and cavity effusion in the fetal stage. We report here on two autopsy cases of HF that were caused by parvovirus B19 (PVB19) infection. The human PVB19 is an erythrovirus that cause diverse clinical manifestations ranging from an asymptomatic or mild presentation to more severe effects such as hydrops fetalis, and this is the only known human pathogenic parvovirus. The gestational ages of the two fetuses were 21 weeks and 23 weeks, respectively. Both fetuses were hydropic and anemic. Hepatic tissues of both fetuses demonstrated erythroblasts with eosinophilic intranuclear inclusions, the so called "lantern cells". PVB19 was confirmed by electron microscopy and immunohistochemical staining. For the diagnosis of this disease, recognition of parvovirus infection as a cause of hydrops fetalis and careful examination of red blood cells with a high-power view are required.
Subject(s)
Humans , Anemia , Autopsy , Diagnosis , Edema , Eosinophils , Erythroblasts , Erythrocytes , Erythrovirus , Fetus , Gestational Age , Hydrops Fetalis , Intranuclear Inclusion Bodies , Microscopy, Electron , Parvoviridae Infections , ParvovirusABSTRACT
Parvovirus B19, a member of the Erythrovirus genus, is the only member of the Parvoviridae family known to be pathogenic in humans. Haematological consequences of B19 infection arise due to a direct cytotoxic effect on erythroid progenitors in bone marrow, resulting in interruption of erythrocyte production. The physiology of host haematopoiesis and competence of the immune response each determines clinical manifestations of B19 infection. A search of the literature revealed that neutropenia and thrombocytopenia due to B19 have been rarely reported in healthy individuals. In Korea, B19 infection has been reported among individuals with underlying disorders such as leukemia or congenital spherocytosis. Among otherwise healthy persons, there have been few reported cases of B19 infection which resulted in anemia, leukopenia, and thrombocytopenia. We herein report, for the first time in Korea, of two children who experienced leukopenia and thrombocytopenia, but not anemia, after B19 infection and recovered uneventfully.