ABSTRACT
The effects of estriol on oxygen uptake, glucose release, lactate and pyruvate production, beta-hydroxybutyrate and acetoacetate production in perfused rat liver as well as, carbon uptake in rat liver and intracellular calcium in isolated Kupffer cells were investigated. Basal oxygen consumption of perfused liver increased significantly in estriol or ethanol-treated rats. But these increased effects were blocked by gadolinium chloride pretreatment. In a metabolic study, pretreatment with estriol resulted in a decrease in glucose production and in glycolysis while an increase in ketogenesis. A more oxidized redox state of the mitochondria was indicated by increased ratios of perfusate [lactate]/[pyruvate] and decreased ratios of perfusate [beta-hydroxybutyrate]/[acetoacetate]. Carbon uptake of Kupffer-cell increased significantly in estriol-treated rats. But these increased uptake were not shown in rats pre-treated by gadolinium chloride blocking phagocytosis. In isolated Kupffer cells from estriol-treated rats, intracellular calcium was more significantly increased after addition of lipopolysaccharide (LPS) than in controls. These findings suggest that the metabolic effects of estriol (two mg per 100 mg body wt) can be summarized to be highly toxic in rat liver, and these findings suggest that oral administration of estrogens may induce hepatic dysfunctions and play a role in the development of liver disease.
Subject(s)
Female , Rats , 3-Hydroxybutyric Acid/metabolism , Acetoacetates/metabolism , Animals , Calcium/metabolism , Carbohydrates/metabolism , Carbon/metabolism , Cells, Cultured , Colloids/metabolism , Estriol/pharmacology , Estriol/metabolism , Ethanol/pharmacology , Gadolinium/pharmacology , Glucose/biosynthesis , Intracellular Fluid/metabolism , Kupffer Cells/metabolism , Kupffer Cells/cytology , Lactates/metabolism , Lipids/metabolism , Liver/metabolism , Liver/drug effects , Oxygen Consumption , Phagocytosis , Pyruvic Acid/metabolism , Rats, Sprague-DawleyABSTRACT
Analise do estriol salivar feita em 18 gestantes, sendo nove gravidas, com OGTT-3hs alterado durante o periodo pre-natal, controladas somente com dieta, classe A de Priscilla White, pertencentes ao grupo um de estudo, e nove gestantes que fizeram uso de insulina durante a gravidez, sendo tres tipo II e seis tipo I (insulino-dependentes), pertencentes ao grupo II de estudo, acompanhadas no pre-natal da Escola Paulista de Medicina no periodo de janeiro de 1987 a julho de 1989. A coleta foi realizada semanalmente, obtendo-se tres amostras, em intervalos de duas horas...