ABSTRACT
A convenient and simple fluorimetric method for the determination of 1-amino-2-propanol, ethanolamine, benzocaine and 4-amino benzoic acid has been developed. The method depends on the reaction of the previously mentioned amines with m-hydroxy benzaldehyde to form highly fluorescent Schiff bases in absolute alcohol at 70C for 20 minutes. On the other hand, these primary amine-containing compounds were determined by colorimetric method. The Schiff bases obtained possess yellow with maximum absorption wave length range of 460-480 nm. Beer's law obeyed in the range of 0.1-5 mg/ml. The effect of pH, temperature, time and solvent of the reaction for both fluorimetric and colorimetric methods were studied and optimized
Subject(s)
1-Propanol/analysis , Ethanolamines/analysis , Benzocaine/analysis , Aminobenzoates/analysisABSTRACT
The endogenous phospholipid acceptor for Ca2+-dependent serine-phospholipid base-exchange reaction was explored in rabbit platelet membranes. When chasing experiments were carried out using prelabeled membranes, cold serine chased labeled serine and ethanolamine, but not labeled choline from corresponding phospholipids. This indicates that phosphatidylserine [PS] and phosphatidylethanolamine [PE] are at least utilized as phospholipid acceptors for serine base-exchange reaction, although it is not certain whether phospha-tidylcholine [PC] is utilized. For the quantitative analysis, the amounts of endogenous bases released from the membranes during serine base-exchange reaction were measured. The serine-dependent release of ethanolamine and choline was only about 10 and 3% of the amount of [3H] serine incorporated into PS, respectively. If Ca2+-dependent serine incorporation into PS occurs only through the so-called base-exchange reaction, these results suggest that the major phospholipid acceptor for serine incorporation is preexisting PS, but not PE or PC in rabbit platelet membranes