Correlation between F2-Isoprostane with stromal cell-derived factor-1 (SDF-1) and endothelial progenitor cell in nonhypertensive and hypertensive patients.

diseases. On the other hand, hypertension is related with excess angiotensin II which would lead to oxidative stress. In this study, we investigated the correlation between F2-Isoprostane (as marker of oxidative stress) with Stromal Cell-Derived Factor-1 (SDF-1) and CD34 viable in non hypertension and hypertension subjects. Methods This was a cross sectional study conducted on 54 nonhypertension and 64 hypertension subjects visiting Prodia laboratory, Jakarta. F2-Isoprostane (as marker of oxidative stress) and SDF-1 (a strmal cell growth factor) were measured by ELISA method, and CD34 viable (marker of progenitor cell) was measured by fl ow cytometry. Results F2-Isoprostane concentration was higher in hypertensive subjects compared to nonhypertensive subjects, although statistically non signifi ant (mean + SD: 0.13 ± 0.120 vs 0.10 ± 0.16; ρg/mL; p = 0.091). SDF-1 concentration was signifi cantly higher in hypertensive subjects compare to nonhypertensive subjects (2821.63 ± 281.94 vs 2623.04 ± 356.28 ρg/mL; P < 0.05). CD34 viable level was signifi cantly lower in hypertensive subjects compare to nonhypertensive subjects (1.9 ± 0.9 /μL vs 2.7 ± 1.7; P < 0.05). F2-Isoprostane had negative correlation with CD34 viable in circulation (r = 0.022, p < 0.05) but no correlation with SDF-1 (p > 0.05). Conclusions F2-Isoprostane was higher, but CD34 was lower, in hypertensive subjects compared to nonhypertensive. It seems that high F2-Isoprostane impaired the CD34 viable level as shown by negative correlation between F2- Isoprostane and CD34.

Influence of acupuncture on isoprostane in patients with Alzheimer's disease / 中国针灸

<p><b>OBJECTIVE</b>To explore the clinical therapeutic effect of acupuncture on Alzheimer's disease (AD) and its mechanism.</p><p><b>METHODS</b>Twenty patients with Alzheimer's disease were treated by acupuncture with reinforcing kidney and activating blood method for 12 weeks and Baihui (GV 20), Shenshu (BL 23), Xuehai (SP 10) and Geshu (BL 17) were selected. The clinical therapeutic effect were assessed by comparing the scores of Alzheimer's Disease Assessment Scale-Cognitive Section (ADAS-Cog) and 8-IPF2alpha concentration in cerebrospinal fluid, blood and urine before and after treatment were detected by using enzyme linked immunosorbent assay.</p><p><b>RESULTS</b>After treatment, the effective rate was 90.0%. The score of ADAS-Cog was 35. 70 +/- 14. 70 before treatment and 31. 45 +/- 4. 08 after treatment, with a significant difference (P<0. 001). The concentration of 8-IPF2alpha in cerebrospinal fluid, blood and urine were all significantly decreased after treatment (all P<0.001).</p><p><b>CONCLUSION</b>Acupuncture can improve the cognitive ability of AD patients and its possible mechanism may be relative to the decrease in lipid peroxidation in AD patients' brain.</p>

Role of 8-isoprostane as an oxidative stress marker in streptozotocin induced diabetic nephropathy in male rats and its correlation with angiotensin II

To evaluate the role of 8-isoprostane as a contributor of diabetic nephropathy [DN], its usefulness as an early marker of oxidative stress, and its correlation with serum and intrarenal angiotensin II [Ang II] production. The study was conducted on 40 age and weight [250-300 g] matched male albino rats. Ten rats were vehicle treated and served as controls, they constituted group I. The remaining 30 rats were made diabetic for 4 weeks by single intravenous streptozotocin [STZ] injection [50 mg/kg]. The diabetic rats were subdivided into 3 groups. Group II: untreated diabetic rats [n=10], group Ill: insulin treated diabetic rats [n=10], they received subcutaneous insulin injections [3 U/rat/day] for 4 weeks. Group IV diabetic rats [n=10], received combined subcutaneous insulin [3 U/rat/day] and intraperitoneal vitamin E [alpha-tocopherol 20 mg/day] for 4 weeks starting from the first day of the experiment. Blood and 24 hours urine samples were collected from all rats at the end of the 1[st], 2[nd], 3[rd] and 4[th] weeks of the study for determination of serum urea, and urinary excretion rates of proteins and 8-isoprostane. At the end of the study period [4 weeks], blood samples were obtained from all rats by cardiac puncture under ether anesthesia. The rats were sacrificed and kidneys were removed for determination of Ang II in kidney tissue homogenates. Final serum glucose, Ang II, and 8-isoprostane were measured. Untreated diabetic rats showed progressive increase in serum urea, proteinuria, and urinary 8-isoprostane excretion rate throughout the whole study, as compared to control ones. Moreover, serum glucose, 8-isoprostane, Ang II, and kidney tissue Ang II were significantly higher in untreated diabetic rats. Insulin treatment significantly reduced the measured parameters in group III rats as compared to group II. A further decrease of the measured parameters was observed after combined insulin and vitamin E treatment in group IV rats. However, the values did not reach basal levels. Increased serum 8-isoprostane and intrarenal Ang II levels as a result of oxidative stress may contribute to the development of DN in hyperglycemic rats. Insulin in combination with vitamin E may exert a more beneficial effect in reducing or retarding the rate of deterioration of diabetic kidney than insulin alone. The advances in the understanding of the 8-isoprostane pathway not only as an early marker of oxidative stress but also as a culprit in the genesis of target organ damage, may lead to novel therapeutic approaches for several diseases resulting from oxidative stress

Oxidatvive Stress in Rat Model of Preeclampsia and Clinical Correlates

There are growing evidences suggesting a pivotal role of oxidative stress in the pathophysiology of preeclampsia. We investigated oxidative stress in the rat model of preeclampsia, and in clinical cases. Pregnant female rats were injected intraperitoneally with deoxycorticosterone acetate (DOCA) and given 0.9% saline as drinking water during their pregnancy. We assessed plasma F2-isoprostane (8-iso-PGF2alpha) and malondialdehyde (MDA) in a rat model, and the same markers in the plasma of maternal blood and fetal cord blood in pregnant women with preclampsia. Blood samples from the umbilical arteries and veins were collected separately. The concentrations of MDA were increased in the preeclampsia groups of animal and humans, compared with the control group; it was significantly increased in the umbilical artery and vein of the preeclampsia group. The concentrations of F2-isoprostane were elevated in the preeclampsia groups of animal and humans, compared with the control group, and the increase in F2-isoprostane concentration was prominent in the umbilical vein than umbilical artery of the preeclampsia group. Therefore, it appears that the placenta has an important role in the pathophysiology of preeclampsia, and the F2-isoprostanes of the umbilical vein may serve as a relatively reliable marker for ischemic/hypoxic injury to the fetus during the perinatal period.