Estructura poblacional y distribución espacial del roble negro (Colombobalanus excelsa, Fagaceae), especie endémica, en fragmentos de bosque andino colombiano / Population structure and spatial distribution of black oak (Colombobalanus excelsa, Fagaceae), endemic species, in Colombian Andean forest fragments

Resumen Los bosques andinos en Colombia son reconocidos por su alta biodiversidad y endemismo, sin embargo han experimentado un severo proceso de fragmentación debido a procesos antrópicos. Como resultado de esto, muchas especies vulnerables han sido particularmente afectadas. Este es el caso del roble negro (Colombobalanus excelsa (Lozano, Hern. Cam., & Henao) Nixon & Crepet), especie endémica y poco estudiada de los Andes colombianos, cuya distribución está restringida a cuatro localidades en el país, una de ellas el sector suroriental del departamento del Huila. Con el fin de estudiar las poblaciones de roble negro en la Serrania de Peñas Blancas (Huila), localidad más sur de distribución; en 2006 se establecieron 16 parcelas de 50 x 20 m (1.6 ha) en fragmentos de bosque, donde se determinó la estructura etaria y la distribución espacial de esta especie. En total se registraron 1 228 individuos de roble negro entre adultos, juveniles y plántulas; los cuáles presentaron patrones de distribución Gamma y Weibull. Con estos resultados se pudo confirmar que la mayoría de los individuos se encuentran en las primeras clases diamétricas. Asimismo, sus poblaciones presentaron un patrón de distribución agregado debido al proceso de extracción de madera. Estos resultados indican que las poblaciones de roble negro se encuentran en una fase de recuperación despúes de una fuerte intervención antrópica. El conocimiento del actual estado de los bosques de roble negro, puede contribuir para el diseño de efectivos planes de conservacion de la especie en Colombia.

Abstract The Colombian Andean forests are recognized for its great biodiversity and endemism; however, they have undergone a severe process of fragmentation due to anthropic factors. As a result, many vulnerable species have been especially affected. That is the case of the black oak (Colombobalanus excelsa (Lozano, Hern. Cam., & Henao) Nixon & Crepet), an endemic and poorly studied species of the Colombian Andes, which has a distribution restricted to only four areas in the country, one of which is the Southeastern Huila region. To examine black oak populations in Serranía Peñas Blancas (Huila), in 2006 we studied the age structure and spatial distribution in plots of 50 x 20 m (1.6 ha) in forest fragments, in southern distribution. In total, 1 228 black oak species were registered among saplings, juvenile and adults, and they have distribution patterns Gamma and Weibull. These results confirm that the majority of the individuals are in the first diametric class. Also, these populations show an added distribution pattern due to the wood extraction process. These results indicate that black oak populations are in a recovery phase after forceful anthropic intervention. Knowing the current status of black oak forests can contribute to the design of effective conservation plans of this species in Colombia. Rev. Biol. Trop. 66(2): 486-494. Epub 2018 June 01.

HPLC analysis of Phenolic Substances and Anti-Alzheimer's Activity of Korean Quercus Species

This study aimed to establish the quantitative method to analyze the content of peroxynitrite-scavengers belonging to polyphenols in six Korean Quercus species (Quercus mongolica, Q. dentata, Q. acutissima, Q. alienta, Q. serrata, and Q. variabilis) by HPLC. The twelve peroxynitrite-scavengers, flavanols (catechins: (+)-catechin, (−)-epicatechin, and (−)-epigallocatechin), flavonols (kaempferol and quercetin), flavonol glycosides (astragalin, quercitrin, and isoquercitrin), flavonol acylated glycosides (astragalin 6″-gallate and isoquercitrin 6″-gallate), gallic acid and its dimer (ellagic acid) were analyzed by HPLC. Further, anti-Alzheimer's activity was assayed in a passive avoidance testusing mice by measuring the retention latency (sec), the concentration of acetylcholine (ACh), and acetylcholinesterase (AChE) activity. Simultaneous analysis of the extracts of the six Quercus leaves was achieved on a Capcell C18 column (5 µm, 250 mm × 4.6 mm i.d.) with a gradient elution of 0.05% HAc and 0.05% HAc in CH₃CN. In the extract of Q. mongolica leaves, the content of gallic acid (32.53 mg/g), (+)-catechin (28.78 mg/g), (−)-epicatehin (22.03 mg/g), astragalin 6″-gallate (20.94 mg/g), and isoquercitrin 6″-gallate (44.11 mg/g) and peroxynitrite-scavenging activity (IC₅₀, 0.831 µg/ml) were high. This extract delayed the retention latency and inhibited acetylcholinesterase activity in scopolamine-induced memory impairment of mice, suggesting that it has anti-Alzheimer's activity.

Quick determination of five sweet constituents in Duosuike Tiancha by RSLC / 中国中药杂志

Duosuike Tiancha contain multiple dihydrochalcone sweet constituents, which are mainly active constituents. For the purpose of overall assessment on quality Duosuike Tiancha, 5 sweet dihydrochalcones in Duosuike Tiancha, phloridzin, phloretin-4'-beta-D-glucopyranoside, 3'-O-acetylphloridzin, 2'-O-acetylphloridzin and phloretin are determined as indicators. The separation was carried out through a isocratic elution using a Waters Acquity UPLC BRH C18 (2.1 mm x 100 mm, 1.7 microm) column and a mobile phase consisting of water (75%) and acetonitrile (25%) at a flow rate of 0.5 mL x min(-1). The detection wavelength was set at 285 nm. The column temperature was 40 degrees C. Under the optimized conditions, all the 5 sweet constituents were successfully separated with in 6 min, and good linearity (r2 > 0.999 1) was achieved. The linear range (g x L(-)) and recoveries were tested with results of 0.022 2-0.444 (98.37%), 0.102 84. 112 (97.32%), 0.003 39-0.067 68 (96.77%), 0.005 1-0.204 (98.85%) and 0.000 538-0.010 76 (100.91%) respectively. The results indicate that the content of the 5 dihydrochalcones were 7.83-62.37, 114.24-272.35, 0-1.02, 0-5.11 and 0.10-1.19 mg x g(-1), respectively. Furthermore, with certain regularity between their content and the sample size, harvest time. The separation and analysis method are fast and simple, as evidenced by the fact that the gradient elution is adopted to rapidly determine one sample within six minutes. Therefore, it can be used for dsetermine 5 sweet dihydrochalcones Duosuike Tiancha.

Xanthophyllomyces dendrorhous (Phaffia rhodozyma) on stromata of Cyttaria hariotii in northwestern Patagonian Nothofagus forests

The occurrence and distribution of Xanthophyllomyces dendrorhous associated with Cyttaria hariotii parasitizing three Nothofagus species (N. dombeyi, N. antarctica and N. pumilio) in northwestern Patagonia (Argentina), as well as the factors that may affect this distribution were herein studied. Between 2000 and 2007, samples were obtained from 18 different locations. Based on physiological tests and morphological characteristics of sexual structures, 72 isolates were identified as X. dendrorhous. Representative strains were studied by MSP-PCR fingerprinting and sequence analysis of the ITS region. MSP-PCR fingerprints were similar for the newly isolated strains, and were also identical to the profiles of the strains previously found in this region. Patagonian strains appear to be a genetically uniform and distinct population, supporting the hypothesis that the association with different host species has determined genetically distinct X. dendrorhous populations worldwide. X. dendrorhous was recovered from N. dombeyi and N. antarctica. Approximately half the sampling sites and samples were positive for X. dendrorhous, but the isolation recovery rate was low. X. dendrorhous was absent in the early stages of ascostromata maturation, becoming more abundant in later stages. The present work represents a step forward in the understanding of the natural distribution and ecology of this biotechnologically relevant yeast.

Xanthophyllomyces dendrorhous (Phaffia rhodozyma) asociado a estromas de Cyttaria hariotii en bosques de Nothofagus en el noroeste de la Patagonia. Se estudió la ocurrencia y la distribución de Xanthophyllomyces dendrorhous asociado a Cyttaria hariotii en tres especies de Nothofagus (N. dombeyi, N. antarctica y N. pumilio) del noroeste de la Patagonia (Argentina), y los factores que podrían afectar esta distribución. El muestreo se realizó entre 2000 y 2007 en 18 sitios diferentes. Según las pruebas fisiológicas y las características morfológicas de las estructuras sexuales, 72 de los aislamientos obtenidos se identificaron como X. dendrorhous. Se estudiaron cepas representativas mediante la técnica de MSP-PCR fingerprinting y secuenciación de la región ITS. Los perfiles de MSP-PCR fueron similares, tanto entre los nuevos aislamientos como entre estos y los de cepas previamente obtenidas en la región. Aparentemente, las cepas patagónicas forman una población genéticamente uniforme y distinta de otras poblaciones. Esto apoya la hipótesis de que la asociación con diferentes especies hospedadoras ha determinado la diferenciación genética de X. dendrorhous en todo el mundo. X. dendrorhous se recuperó de N. dombeyi y de N. antarctica. Aproximadamente la mitad de los sitios de muestreo y de muestras fueron positivos para X. dendrorhous, pero la tasa de aislamiento fue muy baja. X. dendrorhous está ausente en estadios tempranos de maduración de ascostromas y se hace más abundante en estadios más tardíos. El presente trabajo contribuye al mejor entendimiento de la distribución natural y la ecología de esta levadura, de relevancia biotecnológica.

Nutritional and microbiological evaluations of chocolate-coated Chinese chestnut (Castanea mollissima) fruit for commercial use / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

In recent years, China has become an increasingly important and the largest chestnut producer in the world. This study aimed to evaluate the nutritional value and microbiological quality of the roasted freeze-dried Chinese chestnut (Castanea mollissima) (RFDC) coated with dark chocolate (DCC) and milk chocolate (MCC) for industrial use and commercial consumption. Chocolate coating significantly improved the nutritional value of chestnut. RFDC had high levels of starch (66.23%) and fibers (3.85%) while DCC and MCC contained significantly high amounts of sucrose, protein, fat and minerals. Furthermore, the protein content doubled in MCC rather than in DCC. This could be attributed to the different formulations in the two products. Milk powder and whey protein constituted the source of protein in MCC while cocoa powder added to MCC formulation constituted an additional source of minerals. The amino acid profile showed differences in amino acid composition related to the sample's protein content, indicating their good nutritional quality. The moisture contents in all RFDC, DCC and MCC were suitable for industrial processing. These results provide information about the additional nutrients of chocolate-coated chestnut and confirm that the product is an interesting nutritional food. The combination of freeze-drying and chocolate-coating generally results in greater reductions on microbiological loads, extending shelf life of harvested chestnut for commercial application. This is an alternative strategy to add value to chestnut, minimizing the significant losses in harvested fruits and providing a wider range of choices of new products to the consumer disposal.

Study on the genetic diversity of natural chestnut of Shandong by ISSR / 生物工程学报

The genetic diversity of 279 indivdiuals from 10 populations in Shandong Province was investigated using inter-simple sequence repeat (ISSR) markers. As a result, 116 bands were amplified by 10 informative and reliable primers, of which 101 were polymorphic loci. A relatively high level of genetic diversity was revealed: PPL = 87.07, He = 0.2697, H0 = 0.3999 (at the species level); PPL = 64.58, He = 0.2004, H0 = 0.3010 (at the population level). A higher level of genetic differentiation was detected among populations with Nei's G(ST) analysis and the analysis of molecular variance (AMOVA; G(ST) = 0.2414, F(ST) = 0.2224). Habitat fragmentation and gene flow may result in genetic differentiation. UPGMA cluster analysis indicated that the four populations from Linshu, Junan, Tancheng and Feixian grouped together, whereas Laiyang populations clustered in an isolated clade. The results showed that a mixed mating system was possibly the main factor influencing the genetic structure of this species. These results, combined with other information about Castanea mollissima, may provide a valuable basis for proposing conservation strategies.

Chestnut as a Food Allergen: Identification of Major Allergens

Chestnut as a Food Allergen: Identification of Major Allergens To evaluate the clinical significance of chestnut as a food allergen in Korea, skin prick test and ELISA were done in 1,738 patients with respiratory allergies. To identify the IgE binding components, IgE-immunoblotting, 2D IgE-immunoblotting and MALDITOF were performed. To observe the effects of digestive enzymes and a boiling treatment, simulated gastric fluid (SGF) and simulated intestinal fluids (SIF) were incubated with chestnut extracts, and IgE-immunoblotting were then repeated. Skin prick test revealed that 56 (3.2%) patients showed more than 2+ of allergen to histamine ratio to chestnut. Among the 21 IgE binding components, 9 bands were found in more than 50% of the sera tested and the 24 kDa protein had the highest binding intensity. The amino acid sequence of the 24 kDa protein (pI 6.3) had homology with legume protein of oak tree. SGF, SIF and boiling treatment were able to suppress the IgE binding components. In conclusion, chestnut ingestion was shown to induce IgE mediated responses with a 3.2% sensitization rate. Twenty one IgE binding components and one new allergen (the 24 kDa protein) were identified. Digestive enzymes and boiling treatment were able to decrease the allergenic potency.

A new lignan glycoside from the flower of Castanea mollissima Blume / 药学学报

<p><b>AIM</b>To study the bioactive constituents of the flower of Castanea mollissima Blume.</p><p><b>METHODS</b>Compounds were isolated and purified by column chromatography of silica gel and TLC. Structures were determined by various spectroscopic data, including IR, 1HNMR and 13CNMR, EIMS, FABMS and HMBC as well as comparison of the data with those reported in literatures.</p><p><b>RESULTS</b>Five compounds were isolated and elucidated as myricetin (I), quercetin (II), gallic acid (III), 4-quinolinone-2-caboxylic acid (IV), (+) -isolariciresinol-9'-O-alpha-L-rhamnoside (V).</p><p><b>CONCLUSION</b>These compounds were separated from the flower for the first time and compound V is a new compounds, named chestnutlignansoide.</p>

Two new flavonoids from the flower of Castanea mollissima Blume / 药学学报

<p><b>AIM</b>To study the bioactive constituents from the flower of Castanea mollissima Blume.</p><p><b>METHODS</b>Compounds were isolated and purified by column chromatography on silica gel and polyamide. Structures were determined by various spectroscopic data,including UV, IR, 1H and 13CNMR, EIMS and FABMS, 1H-13C-COSY and HMBC.</p><p><b>RESULTS</b>Two compounds were isolated from the ethyl acetate fraction of 95% ethanol extract and the structures were elucidated as kaempferol-3-O-[6"-(E)-p-coumaroyl]-alpha-D-mannopyranoside (1) and kaempferol-3-O-[6", 4"-di-(E)-p-coumaroyl]-alpha-D-mannopyranoside (2).</p><p><b>CONCLUSION</b>Compounds 1 and 2 are new compounds, named castanoside A and B respectirely.</p>

effect of mouth wash extracted from Qureucus Infectoria on dental plaque formation

The aims of this study is to: 1: evaluate clinically the effect of the newly mouth wash extracted from Qureucus infectoria on dental plaque formation. 2: estimate the toxic effect of the extract. 3: assess the antibacterial activity of the extract. The 12 dental students volunteers participating in this trial. The participants were exposed to repeated professional plaque control to eliminate signs of gingivitis and was subsequently told to abstain from mechanical plaque control efforts for the next 5 days they were asked to rinse twice daily for one minute each time with 10 ml of placebo mouth rinse and 0.2% chlorohexidine and three times daily for one minute each time with 10 ml of 1% alcoholic extraction of Qureucus infectoria mouth rinse result demonstrate that plant extract reduced plaque accumulation better than of chlorohexidine mouth rinse