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1.
Yakhteh Medical Journal. 2005; 6 (24): 212-217
in English | IMEMR | ID: emr-75528

ABSTRACT

The aim of this study was to evaluate the relation between protamine deficiency assessed by CMA3 and protamine 1/ protamine 2 [P1/P2] ratio. This study was carried out on 71 patients referring to Isfahan Fertility and Infertility center. Semen analysis was assessed according to WHO criteria. CMA3 staining was use to determine protamine deficiency. P1/P2 ratio was evaluated by nuclear protein extraction, acetic acid urea poly acrylamide gel-electrophoresis and analysis of protein bands with related software. Western blot was carried out with primary anti P1 and anti P2 antibody to determine protamine 1 and protamine 2. Of the 71 patients 45 patients underwent Intracytoplasmic Sperm Injection [ICSI]. A negative significant correlation was observed between fertilization rate with protamine deficiency and P1/P2 ratio. However, no significant correlation was observed between protamine deficiencies with P1/P2 ratio. The results of this study showed that protamine deficiency can be assessed by CMA3; however this procedure does not indicate type of protamine deficiency


Subject(s)
Humans , Protamines/physiology , Chromomycin A3 , Semen/analysis , Fertilization/metabolism , Sperm Injections, Intracytoplasmic
2.
Yakhteh Medical Journal. 2005; 6 (4): 232-236
in English | IMEMR | ID: emr-81564

ABSTRACT

The aim of this study was to evaluate the effect of sperm protamine deficiency on fertilization and embryo development post ICSI. Semen samples from 27 patients candidate for ICSI were assessed for semen parameters. Sperm processing was carried out using pure sperm and percentage of protamine deficient sperm was assessed by Chromomycin A3 post processing. Correlation between fertilization rate, embryo quality and cleavage score with protamine deficiency was assessed using SPSS statistical programme. A significant negative correlation was observed between fertilization rate and embryo quality score on day 3 with percentage of protamine deficient sperms. Semen samples with high percentage of protamine deficient sperms have lower fertilization rate and lower potential to develop good quality embryos


Subject(s)
Humans , Protamines/physiology , Fertilization/metabolism , Sperm Injections, Intracytoplasmic , Embryonic Structures , Semen/analysis
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