ABSTRACT
Los tumores sólidos para crecer más de 2 mm, necesitan desarrollar nuevos vasos sanguíneos. Las células neoplásicas secretan factores de crecimiento que estimulan la angiogénesis y el crecimiento tumoral. La Carragenina bloquea un vitro la unión de los factores de crecimiento a sus receptores. Ensayamos in vivo su acción con el objetivo de analizar si inhibe el desarrollo de un fibrosarcoma murino. Para neutralizar la acción inflamatoria de la Carragenina usamos la Indometacina, que es un antiinflamatorio no esteroide. El tumor usado fue en fibrosarcoma inducido con metilcolanterno en ratones Balb/c y mantenido por pasaje seriado de células tumorales, en ratones de la misma cepa. El volumen de los tumores fue evaluado midiendo dos dimensiones y aplicando la fórmula V = 0.4 x d(2) x D. Los ratones con tumores fueron separados en grupos, uno de los cuales se usó como testigo y los otros tratados en forma separada con indometacina, Carragenina y Carragenina-Indometacina. Se comparó el volumen de los tumores de los ratones tratados con respecto al testigo y el de los tratados entre sí, utilizando el Test t de Student. Se demostró que la Carregenina y la indometacina, inhiben el desarrollo del fibrosarcoma. La acción inhibitoria de la Carragenina sobre el crecimiento tumoral es significativamente mayor que el efecto antitumoral de la Indometacina y el de la Corragenina-Indometacina.
Subject(s)
Animals , Mice , Male , Female , Anti-Inflammatory Agents, Non-Steroidal/antagonists & inhibitors , Carrageenan/pharmacology , Fibrosarcoma/drug therapy , Indomethacin/antagonists & inhibitors , Receptors, Growth Factor/drug effects , Carcinogens , Carrageenan/therapeutic use , Disease Models, Animal , Fibrosarcoma/chemically induced , Fibrosarcoma/metabolism , Methylcholanthrene , Mice, Inbred BALB C , Neoplastic ProcessesSubject(s)
Mice , Animals , Salmonella Infections , Salmonella Infections/therapy , Salmonella typhimurium , Yogurt , Diarrhea/therapy , Fibrosarcoma/chemically induced , Intestinal Mucosa/drug effects , Lactobacillus/immunology , Lacticaseibacillus casei , Nutrition Disorders/therapy , Adjuvants, Immunologic/therapeutic use , Immunocompromised Host , Immunoglobulin A , Vaccines , Adrenal Cortex Hormones/adverse effects , Anti-Bacterial Agents/adverse effectsABSTRACT
45 male albino rats were used to study the effect of deep submucosal implantation of DMBA on lingual carcinogenesis. The results showed development of different mesenchymal neoplasm [fibroma, fibrosarcoma and leiomyosarcoma]. So, this method of implantation could not be used as an ideal animal model for production of single known neoplasm as had been investigated by many investigators before
Subject(s)
Animals, Laboratory , Tongue Neoplasms/chemically induced , Mesoderm/drug effects , Rats , Fibroma/chemically induced , Fibrosarcoma/chemically induced , Leiomyosarcoma/chemically inducedABSTRACT
O comportamento do gerbil (Meriones unguiculatus) como modelo em oncologia experimental é estudado. Aplicaçöes tópicas de 3-metilcolantreno (MC), sobre a pele do dorso, induziram pailomas, carcinomas epidermoides, carcinomas basocelulares e lesöes hiperpigmentadas semelhantes ao nevus azul. Ao contr rio, somente lesöes pigmentadas foram observadas nos animais tratados com MC (iniciaçäo) associado ao óleo de croton (promoçäo). Além disoo, fibrossarcomas e um linfoma foram induzidos com injeçöes subcutâneas de MC.
Subject(s)
Animals , Female , Lymphoma, Non-Hodgkin/chemically induced , Fibrosarcoma/chemically induced , Methylcholanthrene/administration & dosage , Carcinoma, Squamous Cell/chemically induced , Skin Neoplasms/chemically induced , Croton Oil/administration & dosage , Time Factors , Lymphoma, Non-Hodgkin/ultrastructure , Gerbillinae , Nevus, Blue/chemically induced , Fibrosarcoma/ultrastructure , Carcinoma, Squamous Cell/ultrastructure , Disease Models, Animal , Skin Neoplasms/ultrastructureABSTRACT
The two types of transplantable methyl cholanthrene induced fibrosarcoma in rats was used to find out the possible relationship between the fibrinolytic, procoagulant activities and the metastasizing capacity of the tumours. The highly metastatic tumour seems to possess high fibrinolytic activity as compared to the low metastatic one. Interestingly enough, it was found that the procoagulant and fibrinolytic activities in the highly metastasising tumour bear inverse relationship with each other in relation to time of tumour growth. The procoagulant activity of the tumour bypasses factor VII and acts at factor X level. The plasminogen activator present in the tumour tissue has been characterized by sephadex G-200 column chromatography and PAGE.