ABSTRACT
Exposure of rats to 0.1, 0.15 and 0.20 mg/100 g body weight edifenphos (i.p.) had no significant effect on the number of healthy follicles and atretic follicles in all the stages. However, treatment with 0.25 mg dose resulted in a significant decrease in stage I and total number of healthy follicles and increase in stage V atretic follicles. A significant decrease in stage I, II, III and total number of healthy follicles, and a significant increase in stage I, III, IV and total number of atretic follicles were observed in 0.3 mg edifenphos treated rats. The results indicate that the effect of edifenphos is dose dependent.
Subject(s)
Animals , Dose-Response Relationship, Drug , Female , Follicular Atresia/drug effects , Fungicides, Industrial/administration & dosage , Organothiophosphorus Compounds/administration & dosage , Ovarian Follicle/drug effects , Rats , Rats, WistarABSTRACT
Degenerative changes in membrana granulosa of ovaries in R. rattus have been studied using scanning electron microscopy. Ovaries from rats treated with atropine (300 mg/kg body weight) and testosterone propionate (10 IU) were used to study sequential course of atresia in granulosa cells. Granulosa cells undergoing atresia showed degenerative changes in following order i) loosening of intercellular matrix, ii) changed morphology and texture of secretory granules, iii) destabilization of granulosa cell membranes, iv) erosion of cell membrane, v) formation of specific degenerative belts, vi) pycnosis, vii) ghost cell formation and their subsequent mixing in hazzy follicular fluid of cyst. Phenomenon of atresia, its duration, course and underlying causes have been discussed.
Subject(s)
Animals , Atropine/pharmacology , Estrus/drug effects , Female , Follicular Atresia/drug effects , Granulosa Cells/drug effects , Microscopy, Electron, Scanning , Ovary/cytology , Rats , Testosterone/pharmacologyABSTRACT
Surface membrane changes in the granulosa cells from follicles at different times following a single dose of PMSG have been investigated after the incubation of cells in media with and without hCG by spectrophotometric measure of concanavalin A-induced cellular agglutination rate. Agglutination rate and final level of agglutination of cells do not change from 24 to 48 hr but significantly rise at 72, 96 and 120 hr after PMSG administration. Incubation of cells in presence of hCG for 20 min decrease the agglutination level of cells at 24, 48 and 72 hr and no significant change was observed at 96 and 120 hr. The results, thus suggest that atretic changes when induced in the surface membrane of granulosa cells (after 72 hr of PMSG administration) cannot be reversed with hCG.