Effect of the dose of equine chorionic gonadotrophin [ECG] on ovarian response and embryo recovery in Egyptian baladi goats

The effects of doses of equine chorionic gonadotrophin [eCG] for a simple superovulation method in does [Goats] were investigated on ovulation rate, proportions of fertilized oocytes and normal embryos at recovery. The does were treated with intravaginal sponges impregnated with medroxy progesterone acetate [MAP] for 12 days. For superovulation, an intramuscular injection of eCG was given at 24 h before sponge removal. Does were divided into nine equal groups [n = 5] A, B, C, D, E, F, G, H and I. Each received 100, 200, 300, 400, 500, 600, 700, 800 and 900 IU of eCG respectively. All does were naturally inseminated by four healthy fertile bucks. Laparotomy and embryo recovery were done for does on day six. The mean numbers of corpora lutea of nine groups were [2.8 +/- 4.34, 4.4 +/- 0.55, 2.8 +/- 1.10, 4.2 +/- 1.79, 6.8 +/- 2.39, 9.6 +/- 4.45, 6.8 +/- 5.81, 6.6 +/- 3.05 and 5.8 +/- 5.17] respectively. While mean of unovulated follicles were counted after laparotomy for nine groups respectively [3.4 +/- 1.14, 5.8 +/- 1.30, 7.0 +/- 2.35, 5.6 +/- 1.82, 6.8 +/- 2.05, 7.2 +/- 1.92, 9.8 +/- 4.71, 11.2 +/- 5.02 and 10.6 +/- 3.58]. On the other hand the percentage of normal embryos were 0, 15.0, 42.86, 42.11, 54.84, 66.67, 48.64, 38.0 and 45.24] of normal embryos. The present study indicates that an appropriate eCG dose would be 600-700 IU for a simple superovulation method in does pre-treated with Progestagen-impregnated vaginal sponges

Effect of recombinant somatotropin with or without PMSG on postpartum multiparous buffalo in Egypt

This study was carried out on ten Egyptian female multi parous buffaloes suffering from prolonged postpartum period with no estrous signs and low serum progesterone level. Animals were randomly divided into two groups. Females in both groups were injected subcutaneously with 500 mg recombinant bovine somatotropin "rbST",one group received a single intramuscular dose of PMSG [2000 i.u.] 7 days later. Blood samples were collected one week before treatment and for four consecutive weeks after treatment. The levels of progesterone, insulin-like growth factor- 1[IGF-1] and total cholesterol were analysed. Injection of rbST with or without PMSG elevated serum level of IGF-1 for the first two weeks post-injection then declined during the 3rd and 4th weeks compared to pretreatment levels. Both serum progesterone and total cholesterol levels increased throughout the 4 weeks post-injection as compared with their corresponding control values before treatments. In addition, rbST increased milk yield significantly as well as motivated the females to resume cyclic activity of the ovaries [4 / 5 in animals treated with rbST plus PMSG and 3/5 in those treated with rbST alone]. The conception percent improved