ABSTRACT
Study was carried out to understand and compare architecture of the proteins of erythrocyte cell surface of some mammals viz., Homo sapiens (human), Sus scorfa domestica (pig) and Bos taurus domestica (cow). In this study, we investigated the action of proteinases viz., trypsin and chymotrypsin and neuraminidase on the erythrocyte surface proteins and erythrocyte agglutination tendency with a lectin (concanavalin A). The electrophoretic pattern of membrane proteins and glycophorins (analyzed by SDS-PAGE and visualized by Coomassie brilliant blue and periodic acid-schiff stains, respectively) and concanavalin A (Con A) agglutinability revealed that: (i) There were variations in the number and molecular weights of glycophorins in human, pig and cow, (ii) trypsin action on pig and cow erythrocyte membrane proteins was similar, unlike human, (iii) glycophorins degradation by trypsin and chymotrypsin was not similar in pig, as compared to that of human and cow, (iv) erythrocytes agglutination with Con A was significantly different due to differences in membrane composition and alterations in the surface proteins after enzyme treatment, (v) a direct correlation was found between degradation of glycophorins and Con A agglutinability, and (vi) removal of erythrocyte surface sialic acids by neuraminidase specifically indicated an increase in Con A agglutinability of pig and cow erythrocytes, similar to human.
Subject(s)
Animals , Cattle , Cells, Cultured , Concanavalin A/metabolism , Erythrocyte Membrane/drug effects , Erythrocyte Membrane/metabolism , Hemagglutination/drug effects , Hemagglutination/physiology , Humans , Membrane Proteins/metabolism , Peptide Hydrolases/pharmacology , SwineABSTRACT
The state of Pará encompasses 26% of Brazilian Amazon where an enormous diversity of arboviruses has been found. This study aimed to assess the prevalence and distribution of hemagglutination-inhibition antibodies against antigens of six Flavivirus (yellow fever virus, Ilheus virus, Saint Louis encephalitis virus, Cacipacore virus, Bussuquara virus and Rocio virus) in water buffaloes in Pará state, Brazil. The prevalence of antibodies in these farm animals is important to determine the circulating arboviruses.
Subject(s)
Animals , Antibodies/analysis , Flavivirus , Yellow Fever/pathology , Hemagglutination/physiology , Buffaloes/classificationABSTRACT
The state of Pará encompasses 26% of Brazilian Amazon where an enormous diversity of arboviruses has been found. This study aimed to assess the prevalence and distribution of hemagglutination-inhibition antibodies against antigens of six Flavivirus (yellow fever virus, Ilheus virus, Saint Louis encephalitis virus, Cacipacore virus, Bussuquara virus and Rocio virus) in water buffaloes in Pará state, Brazil. The prevalence of antibodies in these farm animals is important to determine the circulating arboviruses.
Subject(s)
Animals , Antibodies/analysis , Yellow Fever/pathology , Flavivirus , Hemagglutination/physiology , Buffaloes/classificationABSTRACT
Se realizó un muestreo en el rastro de Ferrería ubicado al poniente de la zona metropolitana. A ese lugar llegan animales con un peso de 100 a 105 kilogramos que proceden de los estados de Jalisco, Michoacán, Sonora y Guanajuato, con el fin de que se les explore en busca de anticuerpos contra el virus de la influenza porcina (IP). Se acudió al rastro una vez a la semana, durante dos meses, y se colectó un total de 948 sueros; estos últimos se trabajaron con la técnica de microtitulación de la inhibición de la hemaglutinación (IHA). Se consideró positivos los sueros cuyos títulos fueran mayor o igual a 1:80. Los resultados obtenidos se expresaron en porcentajes de animales positivos y negativos, encontrándose anticuerpos en 601 cerdos (63.40 por ciento) con títulos mayores de 1:10, el resto (347) no presentó anticuerpos. De esta manera 20.25 por ciento presentaron títulos mayores de 1:80 (positivos) contra 70.75 por ciento de títulos menores (negativo)