ABSTRACT
The incidence of dengue infection at King Chulalongkorn Memorial Hospital from 1997 to 2000 was reviewed from laboratory information. The highest incidence of the disease occurred from the rainy season to the winter. Most cases were aged below 15 years (87.89%) and no sexual preponderance was observed. The majority of cases were secondary infection (62.09%). Detection of HI titer is still useful for diagnosis although paired serum has to be taken. The time interval between sera should be at least 7 days for suspected primary infection but a shorter time interval can be considered for suspected secondary cases. Rapid and accurate detection of dengue IgM has become more useful because only single serum is required. A request for dengue IgM should be done after day 5 of illness. However, interpretation of the result should be done carefully according to the timing of serum collection.
Subject(s)
Adolescent , Adult , Age Distribution , Antibodies, Viral/analysis , Child , Child, Preschool , Dengue/diagnosis , Female , Hemagglutinins, Viral/analysis , Humans , Immunoglobulin M/blood , Incidence , Infant , Male , Retrospective Studies , Risk Factors , Sensitivity and Specificity , Sex Distribution , Thailand/epidemiologyABSTRACT
Single radial immunodiffusion (SRD) assays were used for measuring the haemagglutinin antigen contents of equine influenza vaccine prepared from an Indian virus isolate. A/Equine-2/Ludhiana/1/87 (H3N8). Five different preparations of the vaccine were standardized by SRD to prepare 913 doses, each containing 20 micrograms HA/ml-1 dose-1. This test also showed influenza virus subtype specificity as no cross reaction was observed between subtype 1 (H7N7) and subtype 2 (H3N8) viruses.
Subject(s)
Animals , Hemagglutinins, Viral/analysis , Horse Diseases/prevention & control , Horses , Immunodiffusion/methods , Influenza A virus/immunology , Influenza Vaccines/analysis , Orthomyxoviridae Infections/prevention & control , Reference Standards , Vaccines, Inactivated/analysisABSTRACT
The use of erythrocytes (RBC) from Presbytis cristata, P. melalophus and Macaca fascicularis for measles hemagglutination inhibition serologies is presented. These primates can provide a ready supply of RBC for diagnostic reagent use in areas of the world where the African green monkey or rhesus macaque are not available. Individuals of each species are useful but must be screened for hemagglutination ability of their erythrocytes. The animal can then be chosen that has erythrocytes which best agglutinate with the antigen being used.