Effect of Aqueous Leaf Extract of Acalypha wilkesiana on Hematological Parameters in Male Wistar Albino Rats.

Aims: The aim of this study is to evaluate the effect of aqueous leaf extract of Acalypha wilkesiana on hematological parameters in male wistar rats. Study Design: In_vivo. Place and Duration of Study: Department of Biochemistry, Faculty of Natural Sciences, Ambrose Alli University, Ekpoma Nigeria, between August 2011 and October 2011. Methodology: Thirty two male wistar rats of average body weights 167.50g were grouped into four (I-IV), of eight rats each. Group I received distilled water (control), while constituted doses of 2500, 5000 and 10000 mg/kg body weight of the extract were administered once daily for 14 days to animals in group II, III and IV respectively. The effect of administration of this extract on hematological parameters was evaluated. Results: Results showed that the extract did not exhibit any significant effect (P>0.05) on packed cell volume, hemoglobin, red blood cell count, white blood cell, neutrophil, lymphocytes, platelets, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and erythrocyte sedimentation rate at all the administered doses of the extract. There was a significant reduction in mean corpuscular volume at all doses of the administered extract when compared with the control. Conclusion: The extract may be considered relatively hematotoxic at a dose of 2500 mg/kg due to its potentials to cause the formation of microcytic RBC’s.

An anthropometric and hematological comparison of sickle cell disease children from rural and urban areas.

BACKGROUND: Sickle cell disease (SCD) is a prevalent genetic disorder in India and the rural and urban areas experience distinctly different healthcare facilities. In view of this, a comparative study of SCD-SS pattern children of age 8–15 years from rural and urban areas of Wardha district of Central India was carried out using anthropometric and hematological parameters. MATERIALS AND METHODS: The data were collected using standard methods and the results showed a significant (P < 0.05) difference in the mean values for body weight, body mass index (BMI), hemoglobin, hematocrit, and white blood corpuscles (WBC). Statistical analysis of the data was done using SPSS 18.0 software. Individuals were screened by solubility test method. Sickle cell patterns (AS and SS) were determined by using electrophoresis technique. RESULT: The SCD-SS children from rural were significantly underweight than those from the urban area of Wardha district. BMI is a good indicator of nutritional status and BMI values of SCD children have less than desired. CONCLUSION: The study highlights an urgent need to conduct integrated investigations for SCD population of rural areas covering clinical, nutritional, and social aspects.

Haematocrit values in ischemic heart disease

Haemoglobin [g/dl] and haematocrit [%] values were determined in 30 healthy controls and 40 patients with ischaemic heart disease. Both groups were non-smokers. Haemoglobin and haematocrit values [mean +/- SE] in patients were 15.65 +/- 0.18 and 46.45 +/- 0.49 and in controls 14.30 +/- 0.14 and 41.50 +/- 0.23. Significantly higher [P< 0.001] haematocrit value by influencing the blood viscosity may impose extra burden on the ischaemic heart

Valores normales de hemoglobina y hematócrito en niñas sanos de 9 a 12 años de edad de la ciudad de Hermosillo, Sonora, México / Normal values of hemoglobin and hematocrit in healthy children 9 to 12 years old in Hermosillo, Sonora, Mexico

Se determinaron los valores de hemoglobina (Hb) y hematócrito (Hto) en niños sanos escolares (9 a 12 años) de la ciudad de Hermosillo, Sonora, a partir de un total de 797 niños (5 a 15 años) como muestra inicial. Se seleccionaron los que cumplían los siguientes requisitos: residencia de seis meses, porciento peso para la talla entre 90-110% de la media de Ramos-Galván, ausencia de antecedentes infecciosos y de sangrado en los dos meses previos, no antecedentes de recibir medicamentos 15 días antes, niveles de proteína sérica superior a 5.0 g/dl (50 g/l), hierro sérico superior a 50.0 microng/dl (8.955 micronmol/l), examen de orina normal, estudios de materia fecal para sangre oculta negativos y ausencia de parásitos. Quedaron 180 sujetos entre 9 y 12 años y se eliminaron 50 por no cumplir con alguna de las restricciones del protocolo; por lo tanto, se estudiaron 130, 64 del sexo masculino y 66 del femenino, quedando la muestra por arriba del 99% de confiabilidad y un error máximo del 1%. Después de la primera determinación de Hb y Hto, se suplementó con hierro, ácido fólico y vitamina B12 durante 4 semanas; posteriormente, se determinó Hb y Hb nuevamente. No se encontró diferencia significativa (p <0.01) con respecto a los iniciales. El valor medio de Hb y Hto fue de 13.4 g/dl (8.316 mmol/l) y 41% respectivamente

Variaciones del hierro, cinc y cobre del plasma en pacientes reumáticos / Serum iron, zinc and copper changes in rheumatic patients

En este trabajo se trata de establecer las variaciones de algunos parámetros vinculados al metabolismo del hierro (sideremia, TIBC, saturación porcentual y transferrina) y del cobre y cinc séricos en 50 pacientes reumáticos de ambos sexos, con medicación y sin ella. En las enfermedades reumáticas se producen modificaciones en la concentración de algunos oligoelementos (Fe, Cu, Zn, Mg, etc.) por hallarse involucrados en gran número de procesos del metabolismo tisular, especialmente en el tejido conectivo. Las patologías estudiadas fueron: artritis reumatoidea, artrosis, osteoartrosis y espondilitis. El hierro plasmático dio un promedio de 85,96 + ou - 3,5 microng%; la TIBC 224,31 + ou - 9,9 microng%; transferrina 0,17 + ou - 0,007 g% y saturación: 34 + ou - 1,45%, significativamente inferiores (P < 0,05) a los valores normales. También se hallaron disminuidas las concentraciones de cinc y cobre séricos: 63,81 + ou - 5,5 y 73,41 + ou - 3,9 microng% respectivamente (P < 0,05). El tratamiento (un año) no modificó los parámetros estudiados