ABSTRACT
Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.
Subject(s)
Animals , Female , Mice , Actinobacillus Infections/microbiology , Actinobacillus pleuropneumoniae , Antigens, Bacterial/immunology , Bacterial Proteins/immunology , Bacterial Vaccines/immunology , Cholera Toxin/chemistry , Hemolysin Proteins/immunology , Immunization, Secondary , Mice, Inbred ICR , Plants, Genetically Modified , Zea mays/geneticsSubject(s)
Humans , Adult , Antibodies/immunology , Antigens/isolation & purification , Erythrocyte Membrane/immunology , Acute Kidney Injury/immunology , Antigens, Neoplasm/immunology , Bacterial Infections/immunology , Erythrocyte Aggregation/immunology , Hemolysin Proteins/immunology , Lectins/immunology , Membrane Proteins/immunologyABSTRACT
Specific antisera to V. cholerae El Tor hemolysin were prepared. The sera exhibited the following characteristics: formed a single precipitin band in immunoelectrophoresis against the crude preparation of hemolysin, had no passive hemagglutinating antibodies against V. cholerae LPS sensitized cells, possessed neutralizing property to the homologous hemolysin, and afforded some small degree of protection to oral challenge of V. cholerae El Tor in experimental animals.
Subject(s)
Animals , Cholera/immunology , Hemolysin Proteins/immunology , Immune Sera/immunology , Immunoelectrophoresis , Lipopolysaccharides/immunology , Mice , Rabbits , Vibrio cholerae/immunologyABSTRACT
Pathological abnormalities of the infant mouse small intestines were evident after V.cholerae El Tor, Ogawa, infection. These were revealed by an enteritis manifested by mononuclear cells containing inflammatory exudate, intervillous blood capillary congestion, marked increase in goblet cells, desquamation of epithelium and diapedesis of cells. Mice received V.cholerae pretreated with high concentration of antihemolysin, though developed diarrhea, had marked decrease in pathological severity. The less amount of the antibodies the more pathological changes were found.