ABSTRACT
El virus de la hepatitis B (VHB), es un virus DNA, el cual tiene varios antígenos, como el antígeno de superficie, y antígeno core. Colombia, es un país de baja endemicidad, sin embargo, en la Sierra Nevada de Santa Marta, está endemicidad es alta. El VHB tiene como una de sus complicaciones la hipertensión porta. En general, el VHB no atraviesa la placenta, por lo que la infección es rara in utero. Son pocos los pacientes que se presentan con HB y falla hepática fulminante y por lo tanto, son pocos los antivirales que han sido utilizados, con muy poca experiencia.
The hepatitis B virus (HBV) is a DNA virus, which has several antigens such as surface antigen and core antigen. Colombia is a country of low endemicity, however, in the Sierra Nevada of Santa Marta, is endemic is high. HBVis one of the complications of portal hypertension. In general, HBV does not cross the placenta, so the infection is rare in utero. Few patients who present with HB and fulminant hepatic failure and therefore, few antiviral drugs that have been u s e d , wi t h v e r y l i t t l e e x p e r i e n c e.
Subject(s)
Humans , Male , Female , Child , Hepatitis B Antibodies/classification , Hepatitis B Antibodies , Hepatitis B/classification , Hepatitis B/complications , Hepatitis B/diagnosis , Hepatitis B/epidemiology , Hepatitis B/etiology , Hepatitis B/genetics , Hepatitis B/pathology , Hepatitis B/prevention & control , Hepatitis B Antibodies/genetics , Hepatitis B Antibodies , Hepatitis B Antibodies/therapeutic use , Hepatitis B/transmission , Hepatitis B/virology , Hepatitis B Vaccines/administration & dosage , Hepatitis B Vaccines/isolation & purification , Hepatitis B Vaccines/classification , Hepatitis B Vaccines/pharmacology , Hepatitis B VaccinesABSTRACT
To identify the distribution pattern of Hepatitis B Virus [HBV] genotype in a group of patients and to study its phylogenetic divergence. The clinics of Gastroenterology Unit, Ziauddin University, from January to December 2006. Two hundred and one HBV infected patients were genotyped for this study. All HbsAg positive individuals, either healthy carriers or suffering from conditions such as acute or chronic hepatitis, cirrhosis and hepatocellular carcinoma were included. Hepatitis B patients co-infected with other hepatic viruses were excluded. Hepatitis B virus DNA was extracted from serum, and subjected to a nested PCR, using the primers type-specific for genotype detection. Phylogenetic analysis was performed in the pre-S1 through S genes of HBV. The divergence was studied through 15 sequences of 967bp submitted to the DBJ/EMBL/GenBank databases accessible under accession number EF584640 through EF584654. Out of 201 patients tested, 156 were males and 45 were females. Genotype D was the predominant type found in 128 [64%] patients followed by A in 47 [23%] and mixed A/D in 26 [13%]. Phylogenetic analysis confirmed the dominance of genotype D and subtype ayw2. There was dominance of genotype D subtype ayw2. It had a close resemblance with HBV strains that circulate in Iran, India and Japan