Foliculitis necrotizante herpética / Necrotizing herpes folliculitis. Report of one case

Herpes folliculitis is a rare manifestation of herpes virus infection. It usually represents a diagnostic challenge, due to the absence of characteristic skin manifestations such as vesicles or pustules. The reported cases are mainly associated with varicella zoster virus (VZV) and less commonly with herpes simplex viruses (HSV-1 y HSV-2). We report a 51-year-old male with a relapsing non-Hodgkin Lymphoma under chemotherapy, with history of extensive follicular lesions lasting one month. The pathologic study of the lesions was consistent with necrotizing herpes folliculitis. The patient was treated with Valacyclovir, achieving remission of the lesions. The appearance of folliculitis, especially in an immunocompromised patient, should raise the suspicion of herpes virus infection. Polymerase chain reaction may help to elucidate the diagnosis when pathologic findings are non-specific.

Detección de VPH y expresión: de p53 en carcinoma de cabeza y cuello / HPV detection and expression: of p53 in head and neck carcinoma

El cáncer de cabeza y cuello ha sido frecuentemente asociado al abuso en el consumo del alcohol y tabaco, aunque existe un bajo porcentaje de casos que no presenta una historia de alcohol y/o tabaco conocida. Esto ha llevado a considerar la exposición a otros factores de riesgo como por ejemplo el virus de papiloma humano. Actualmente los oncólogos evalúan distintos marcadores oncogénicos, entre ellos la proteína p53, lo cual les permite realizar el pronóstico y diagnóstico más preciso. El objetivo de este estudio fue evaluar la infección de virus de papiloma humano en pacientes con carcinoma de células escamosas de cabeza y cuello mediante reacción de cadena polimerasa, así como la detección de p53 por inmunohistoquímica. De 30 muestras evaluadas, 12 (40 por ciento) resultaron positivas para la detección viral, de éstas el 58 por ciento correspondía virus de papiloma humano tipo 11 (de bajo riesgo oncogénico), siendo la cavidad bucal la localización anatómica más afectada por la presencia del genoma viral. El p53 fue encontrado en 50 por ciento, siendo la orofaringe la localización anatómica con mayor positividad para dicho marcador.

The head and neck cancer has been frequently associated with the alcohol and tobacco abuse consume, although there are a low percents of cases that have not an alcohol and tobacco history know. This has made to consider another risks exposure factors how for example, the human papilloma virus. At present the oncologist evaluate different oncogenic markers inside them like the p53, this permit to them realize a better prediction and the prognostic. The objective of this study was evaluating the human papilloma virus infection in the patients with diagnostic of head and neck carcinoma using the polymerase reaction chain, such as the detection of the p53 by the immunohistochemistry procedure. Of the 30 evaluated samples 12 (40 %) of them result positive to the human papilloma viral detection, of these the 58 % correspond to the human papilloma virus type 11 (low oncogenic risk), been the oral cavity the anatomic localization most affected by the viral genome presence. The p53 has been finding in the 50 %, the orofaringe was the anatomic localization who has most positive to the marker.

Estudio de infección sistémica por herpesvirus complicada con Cryptosporidium spp: en un delfín manchado del atlántico (Stenella frontalis Curvier 1829) / Study of systemic infection by herpesvirus complicated with Cryptosporidium spp: in a gulf stream spotted dolphin (Stenella frontalis Curvier 1829)

El presente estudio se realizó a partir de un caso de veramiento de delfín Stenella frontalis, hembra cría, reportado el 30/07/02 para Playa Dorada. Boca de Aroa, estado Falcón, Venezuela. Se mantuvo en una instalación marina por un período de tres días, tiempo durante el cual se le realizaron evaluaciones periódicas no invasivas y toma de datos morfométricos. Murió el 01/08/02 por paro cardiopulmonar. Se practicó estudio macroscópico postmortem, preservándose muestras en formoldehído al 10 por ciento para estudios histopatológicos de los siguientes órganos: corazón, pulmón, estómago, intestino grueso, hígado, riñón y lesiones de piel con capa grasa, fueron procesados en el laboratorio de Anatomía Patológica de la Universidad Central de Venezuela. Por los resultados obtenidos en el estudio microscópico se determinó que el individuo presentó una infección sistémica producto de una infección primaria por Herpesvirus spp., concominante con parasistismo secundario por Cyptosporidium spp., cuadro clínico que se vio complicado por las condiciones de estrés a las que se encontró sometido, producto del varamiento. Según la revisión bibliográfica realizada, la parasitosis por Cyptosporidium spp. no aparece reportada en Venezuela para el orden: Cetácea, lo cual se evidencia en la información recopilada acerca de parásitos estudiados para dicho orden. La osamenta se encuentra en Museo Científico de la Estación Biológica. Ranchi Grande-MARNR.

The present study was made, in the event of a young female dolphin of the specie Stenella frontalis, that was found stranded in Playa Dorada, in Boca de Aroa, in the Falcón State, the 07/30/02, in Venezuela. She was kept in a facility for a period of three days, during this period of time, she was evaluated periodically whit not invasive technique, and morphometry data recollection. She died the 08/01/02 due to respiratory arrest, which caused a cardiac arrest. A macroscopic postmortem study was made, taking samples and keeping them in formaldehyde 10% for histopathology study of the following organs: heart, lungs, stomach, large intestine, liver, kidney and lesions of the skin with a fat layer, they were processed in the Pathologic Anatomy laboratory of the Universidad Central de Venezuela. Because of the results of the microscopic study, it was determined that the dolphin presented a systemic infection, because of a primary infection by Herpesvirus spp., related with a secondary infection by Cryptosporidium spp., which was complicated with stress due to the beaching. The bibliographic revue reports that the parasitosis by Cryptosporidium spp. is not present to Venezuela in the Cetacean order, according to the compiled information about the studied parasites for this order. The skeleton is currently in the Scientific Museum of the Biological Station Rancho Grande - MARNR.

Pathological and Immunohistochemical Findings in Broilers Inoculated with a Low Virulent Strain of Infectious Laryngotracheitis Virus

This study was carried out to describe clinical, gross and histopathological findings in the respiratory tract in chickens infected intranasally with A96 strain of infectious laryngotracheitis virus (ILTV). In addition, the presence of ILTV antigens in formalin-fixed and paraffin-embedded larynx and trachea tissues was investigated with the immunoperoxidase (IP) method in the infected chickens. At various days of viral infection, nares, larynx, trachea, lungs and air sacs tissue samples of the infected chickens were obtained and fixed with formalin and embedded in paraffin. The cross sections were stained with hematoxylineosin, and the larynx and trachea sections were also stained with the IP method. Mild rales and gasping were observed in only 4 of 35 chickens. The virus caused mild inflammatory changes in the respiratory tract. It was shown that clinical, gross and histopathological findings were not specific for differential diagnosis of the disease. However, ILTV antigens were detected by the IP method in formalin-fixed and paraffin-embedded larynx and trachea sections. These results revealed that the study use of the IP method might be useful for the diagnosis of ILTV infections with non-specific lesions.

Experimental study of mouse cytomegalovirus infected mice / 华中科技大学学报（医学）（英德文版）

In order to investigate the human cytomegalovirus (HCMV) infection, the mouse cytomegalovirus (MCMV) infected mice were experimentally studied. 6 to 8 week old female BALB/C mice with immunosuppression were selected to undergo the MCMV inoculations: intracranial inoculation and peritoneal inoculation. MCMV of the infected mice in various organs and tissues were detected by using beta-gal staining and in situ nucleic acid hybridization assay. The pathological changes were observed in HE staining paraffin-embedded sections. It was found that all the MCMV infected mice showed the retardation of growth and development, and feather looseness. Both intracranial inoculation of 10(4) PFU viruses or peritoneal inoculation of 10(6) PFU viruses resulted in the pathological changes, to some extent, of various organs and tissues in the mice. The pathological changes in liver were consistent with the amount of beta-gal staining positive cells, indicating the liver lesions were mainly caused by viral proliferation. It was also found that the viruses in the immunosuppressed mice subjected to intracranial inoculation could spread to whole body organs, while the viruses in the immunosuppressed mice subjected to intrapeitoneal inoculation couldn't spread to the brain, suggesting blood-brain barrier could prevent the virus from spreading to the brain.

Neonatal hepatitis--an autopsy study of 14 cases.

Fourteen autopsy cases of neonatal hepatitis have been studied. Of these seven cases were due to infections viz.: cytomegalovirus infection (four cases), probable cases of congenital syphilis (two cases) and neonatal herpes (one case). The remaining seven cases were of Idiopathic Neonatal Hepatitis (INH) with giant cell change in six cases. Even in these cases (INH) there was a high index of suspicion of intrauterine or acquired infection in view of severe mononuclear inflammation in the pancreas, alimentary tract and lungs. Most of these neonates with INH had low birth weight and two were preterm pointing towards a prenatal insult. The orcein stain and Periodic Acid Schiff (PAS) with diastase in all the cases were negative making hepatitis B virus infection and infinity 1 antitrypsin deficiency less likely. These autopsies represent the tip of the iceberg and only the severe cases of infection. The fatal outcome could have been prevented by maternal screening for infections and earlier clinical diagnosis.

Sepsis por virus de Epstein-Barr: informe de caso / Sepsis by Epstein-Barr virus: clinical report

Se psesenta el caso clínico de una lactante menor femenino de 7 meses de edad, con una infección severa del tipo sepsis por Virus de Epstein-Barr. Sin antecedentes personales o familiares de enfermedad y/o deficiencia inmunológica. Ingresó con los siguientes hallazgos: Rash petequial generalizado, neumonía derecha, inestabilidad hemodinámica y deterioro progresivo del estado de conciencia, hepatomegalia importante con evaluación significativa del nivel de transaminasas, sin hiperamonemia. Ameritó apoyo ventilatorio y hemodinámico, además de tratamiento con antibióticos y acyclovir parenteral con evolución clínica y paraclínica satisfactoria. Egresó en excelentes condiciones físicas luego de 28 días de permanencia hospitalaria, incluyendo 8 días en UTI pediátrica. La investigación serológica reportó niveles de IGMVCA de 1:1360 y IgGVCA de 1:120 lo cual revela la presencia de anticuerpos específicos contra la cápside viral del EBV correspondientes a una infección primaria. Los estudios inmunológicos normales

Comunicación de caso clínico de encefalitis herpética demostrada por biopsia cerebral con microscopía electrónica / Communication of a clinical case of herpetic encephalitis demonstrated by cerebral biopsy with electronic microscopy

Se presentan los hallazgos de biopsia cerebral realizada en una menor de 2 años de edad, con evidencias clínicas de una encefalitis aguda grave. La biopsia se efectuó en el lóbulo temporal derecho basado en la signología clínica de focalización neurológica y la TAC que demostró mayor compromiso en áreas temporales. Mediante microscopía electrónica se visualizaron múltiples partículas virales intranucleares morfológicamente idénticas al virus herpes simplex. A través del método peroxidasa anti peroxidasa (PAP) se observó una marcada positividad para herpes simplex tipo II. Se realizó tratamiento antiviral con Aciclovir a partir del 6º día de enfermedad. Sobrevivió con grandes secuelas neurológicas