ABSTRACT
Abstract INTRODUCTION: Human herpesvirus (HHV)-7 establishes a latent infection during the lifetime of the host and can reactivate after the primary infection, leading to lytic replication in immunosuppressed patients. METHODS: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) to identify HHV-7 serum antibodies and compare its performance with that of an indirect immunofluorescence assay (IFA). RESULTS: Serum samples (n=102) were tested by IgG-IFA and by ELISA. IFA and ELISA showed IgG-positive results in 77 and 73 samples, respectively. Qualitative concordance of 96% was demonstrated between the two techniques. CONCLUSIONS: ELISA may be useful to diagnose HHV-7 infection.
Subject(s)
Humans , Immunoglobulin G/blood , Herpesvirus 7, Human/immunology , Roseolovirus Infections/diagnosis , Antibodies, Viral/blood , Enzyme-Linked Immunosorbent Assay , ROC Curve , Sensitivity and Specificity , Fluorescent Antibody Technique, IndirectABSTRACT
El herpes virus humano 7 (HHV-7) fue aislado recientemente de las células CD4 de individuos sanos; actualmente se realizan estudios de suposible asociación con enfermedades de humanos. Se estudiaron 200 muestras de sangre de candidatos a donadores asintomáticos del banco de sangre del Hospital General de México, utilizando la prueba de inmunofluorescencia indirecta (IFA) en células SupT1 infectadas con el HHV-7 en la Universidad de Colonia, Alemania. El 83.5 por ciento de las muestras fueron de varones y el 16.5 por ciento de mujeres; provenían de 12 diferentes estados de la república mexicana con predominio del Distrito Federal (60.5 por ciento) y del Estado de México (28 por ciento) con edad promedio de 29.2 años. El HHV-7 fue detectado en el 98.5 por ciento de los casos a diferentes titulaciones; el 84.5 por ciento presentó títulos elevados (ò 1:80). El 1 por ciento resultó positivo a hepatitis B, el 2 por ciento a sífilis, y el 0.5 por ciento a brucella. Todos fueron negativos a hepatitis C así como al VIH. La elevada prevalencia de HHV-7 deberá aclararse en investigaciones futuras que permitan determinar la posible asociación de títulos altos con infección activa, así como el significado de ésta en relación a enfermedad
Subject(s)
Adult , Humans , Male , Female , Antibodies, Viral , Antibodies, Viral/isolation & purification , Blood Donors , Blood/immunology , Fluorescent Antibody Technique , Herpesvirus 7, Human/immunology , Herpesvirus 7, Human/isolation & purificationABSTRACT
Seroprevalence of human herpesvirus 6 (HHV-6) and 7 (HHV-7) was estimated in the Thai population using indirect immunofluorescence assay to determine serum antibodies to HHV-6 and HHV-7. A total of 333 serum samples obtained from umbilical cord blood and venous blood of healthy persons at Siriraj Hospital and Krabi Hospital during 1990-1993 were investigated. Of 73 infants aged 0-1 month, 73% and 78% were found tob e positive for HHV-6 and HHV-7 antibodies, respectively. Antibody to HHV-6 was detected in age groups 2-3 months (38%), 4-5 months (14%), 6 months (44%), 7-11 months (66%), 1-2 year (84%), 3-4 years (82%), 5-9 years (83%), 10-19 years (83%), 20-29 years (80%), 30-39 years (67%), and over 40 years (58%), respectively. This positive rates of HHV-7 antibody in age groups 2-3 months, 4-5 months, 6 months, 7-11 months, 1-2 years, 3-4 years, 5-9 years, 10-19 years, 21-29 years, 30-39 years, and over 40 years were 50%, 21%, 10%, 37%, 47%, 82%, 75%, 72%, 72%, 67%, and 67%, respectively. At 6 months of age as the starting time of infections, 34% (14/41) and 9% (3/41) of infants had presumed primary infections of HHV-6 and HHV-7, respectively. In the follow-up study, 53% (20/38) of children were infected with HHV-6 prior to HHV-7 and only 5% vice versa. Eighty-four percent of children had acquired antibody to HHV-6 by 1-2 years old while 82% of children had acquired antibody to HHV-7 by 3-4 years old. These results suggest that HHV-6 and HHV-7 are prevalent viruses in the Thai population. The infections of both viruses begin at 6 months of age. However, infection of HHV-7 in most children begins later. The data also provided evidence that antigenic distinction between HHV-6 and HHV-7 existed with a limited cross-reactivity in an antibody test. The antibody responses to HHV-6 and HHV-7 occurred independently.