ABSTRACT
PURPOSE: To evaluate an experimental animal model to study the abdominal tissue activity considering its interaction with a polypropylene mesh, through the use of one of the optical phenomena of light Laser, the biospeckle. METHODS: Fifty Wistar male rats were divided into four groups: Group 1: ten animals not submitted to surgery; Group 2: ten animals submitted to surgery without polypropylene mesh; Group 3: 20 animals submitted to surgery followed by the mesh placement; Group 4: (sham) with ten animals. None of the animals presented post surgical complications being submitted to the optical tests at the 20th postoperative day. RESULTS: The analysis from the biospeckle tests, comparing the medians and standard deviations with T Student test, indicated that no significative difference was observed on the abdominal wall tissue activity in the four groups considered, with and without polypropylene mesh prosthesis implantation. CONCLUSION: The animal model is viable and the biospeckle open ways for a great number of experiments to be developed in evaluating tissue activity.
OBJETIVO: Avaliar o modelo experimental animal para o estudo da atividade tecidual da parede abdominal em sua interação com a prótese de polipropileno, através da utilização de um dos fenômenos ópticos da luz Laser, o biospeckle. MÉTODOS: Foram utilizados 50 ratos Wistar machos, divididos em quatro grupos: Grupo 1 com 10 animais não operados e controles; Grupo 2 com 10 animais submetidos a cirurgia sem a colocação de prótese; Grupo 3 com 20 animais submetidos a cirurgia com colocação de prótese de polipropileno pré-peritonial; Grupo 4 (Sham) com 10 animais. Nenhum animal apresentou complicações pós-operatórias, sendo que no 20º PO foram submetidos ao ensaio óptico. RESULTADOS: A comparação das médias e o desvio padrão das medidas da atividade tecidual da parede abdominal, com e sem implantação da prótese de polipropileno obtidas dos grupos de animais, pelo teste T de Student, não evidenciou diferença estatística significativa (p>0.05). CONCLUSÃO: A análise dos dados obtidos permitiu concluir que o modelo animal é viável e que o biospeckle abre caminhos para toda uma linha de experimentos a ser desenvolvida em avaliar atividade tecidual.
Subject(s)
Animals , Male , Rats , Abdominal Wall/surgery , Image Enhancement/standards , Lasers , Optical Phenomena , Polypropylenes , Surgical Mesh/adverse effects , Wound Healing/physiology , Abdominal Wall/pathology , Image Enhancement/methods , Models, Animal , Polypropylenes/adverse effects , Polypropylenes/therapeutic use , Postoperative Complications/pathology , Random Allocation , Rats, Wistar , Tissue Adhesions/pathologyABSTRACT
Delivering the radiation dose to the target volume and minimizing the dose to normal tissues are the main objectives in radiotherapy. The aim of our study is to enhance the contrast of the portal image to increase the accuracy of delineation of the organs in the irradiation field. The software was written based on local enhancement of the pixel values in image matrix. The portal images were digitized by charged coupled device [CCD] in compatible format to be read with this program. This program was applied as an m-file in MATLAB imaging tool box to the matrices of the portal images. The imaging parameters before and after application of the program were compared. The quantitative information of images was obtained. Analysis of the mean and standard deviations of the results has shown that the difference of the criteria between two groups of the images is significant [p< 0.01]. In qualitative analysis, final images scores were based on "special weight ". The result of this test confirms the superior quality of the post-processed images from the professional view point. Superiority of final images within the three studied parameters by the experts [superiority of lung image, superiority of thorax and its soft tissue images] can be used to increase the accuracy of the treatment set up and decrease the probability of normal tissue complications