ABSTRACT
This study aimed to investigate the immediate effects of the secretory immunoglobulin A (sIgA), α-amylase activity and blood pressure levels after the application of a Reiki session in nurses with Burnout Syndrome. A randomized, double-blind, placebo-controlled, crossover design was conducted to compare the immediate effects of Reiki versus control intervention (Hand-off sham intervention) in nurses with Burnout Syndrome. Sample was composed of eighteen nurses (aged 34-56 years) with burnout syndrome. Participants were randomly assigned to receive either a Reiki treatment or a placebo (sham Reiki) treatment, according to the established order in two different days. The ANOVA showed a significant interaction time x intervention for diastolic blood pressure (F=4.92, P=0.04) and sIgA concentration (F=4.71, P=0.04). A Reiki session can produce an immediate and statistically significant improvement in sIgA concentration and diastolic blood pressure in nurses with Burnout Syndrome.
O objetivo deste estudo foi investigar os efeitos imediatos na imunoglobulina A salivar (IgAs), na atividade de α-amilase e na pressão arterial, após uma aplicação de Reiki em enfermeiras que sofrem da síndrome de Burnout. Foi realizado ensaio clínico randomizado duplo-cego e placebo controlado, com desenho cruzado. Dezoito enfermeiras (idade entre 34 e 56 anos), com síndrome de Burnout, participaram do estudo. As participantes receberam tratamento com Reiki ou Reiki falso, de acordo com a ordem estabelecida, através da randomização em dois dias distintos. O teste de Anova mostrou interação significativa entre o momento da intervenção e a pressão arterial diastólica (F=4,92, p=0,04) e os níveis de sIgA (F=4,71, p=0,04). Conclui-se que uma sessão de Reiki de 30 minutos pode melhorar de forma imediata a resposta de IgAs e da pressão arterial diastólica em enfermeiras com síndrome de Burnout.
El objetivo fue investigar los efectos inmediatos en inmunoglobulina A salival (IgAs), actividad de α-amilasa y presión arterial de una aplicación de reiki en enfermeras sufriendo síndrome de Burnout. Se utilizó un ensayo preliminar placebo randomizado con cegamiento doble utilizando un diseño cruzado. Dieciocho enfermeras (edad 34-56) con síndrome de Burnout participaron en el estudio. Las participantes recibieron tratamiento con Reiki o Reiki fingido según el orden establecido por la randomización en dos días distintos. El test de ANOVA mostró un interacción significativa momento intervención para la presión arterial diastólica (F=4.92, P=0.04) a y la concentración de sIgA (F=4.71, P=0.04). Una sesión de Reiki de 30 minutos puede mejorar de manera inmediata la respuesta de IgAs y la presión arterial diastólica en enfermeras con síndrome de Burnout.
Subject(s)
Adult , Female , Humans , Middle Aged , Burnout, Professional/therapy , Nursing , Therapeutic Touch , Blood Pressure , Burnout, Professional/blood , Burnout, Professional/physiopathology , Cross-Over Studies , Double-Blind Method , Immunoglobulin A, Secretory/blood , alpha-Amylases/bloodABSTRACT
Sixty patients were included in this study. Thirty of them were suffering from chronic sinusitis and the others were control. Serum IgA, IgM and secretory IgA were estimated using nephelometry and single radial immunodifussion techniques respectively. These immunoglobulins are responsible for the integrity of the local immune system present in the respiratory mucosa particularly the nasal mucosa. Serum Ig A and serum Ig M were significantly increased in chronic sinusitis group [P<0.05], while secretory Ig A showed an insignificant decrease in the same group [P>0.05]. Those findings indicate that activation of the mucosal immune system e .g. by nasal vaccination might be a useful and promising strategy for preventing upper respiratory infections including nasal infectious diseases
Subject(s)
Humans , Male , Female , Chronic Disease , Immunoglobulin A/blood , Immunoglobulin M/blood , Immunoglobulin A, Secretory/blood , Comparative StudyABSTRACT
Escherichia coli heat-labile enterotoxin (LT) is composed of catalytic A and non-catalytic homo-pentameric B subunits and causes diarrheal disease in human and animals. In order to produce a nontoxic LT for vaccine and adjuvant development, two novel derivatives of LT were constructed by a site-directed mutagenesis of A subunit; Ser63 to Tyr63 in LTS63Y and Glu110, Glu112 were deleted in LT delta 110/112. The purified mutant LTs (mLTs) showed a similar molecular structural complex as AB5 to that of wild LT. In contrast to wild-type LT, mLTs failed to induce either elongation activity, ADP-ribosyltransferase activity, cAMP synthesis in CHO cells or fluid accumulation in mouse small intestine in vivo. Mice immunized with mLTs either intragastrically or intranasally elicited high titers of LT-specific serum and mucosal antibodies comparable to those induced by wild-type LT. These results indicate that substitution of Ser63 to Tyr63 or deletion of Glu110 and Glu112 eliminate the toxicity of LT without a change of AB5 conformation, and both mutants are immunogenic to LT itself. Therefore, both mLTs may be used to develop novel anti-diarrheal vaccines against enterotoxigenic E. coli.
Subject(s)
Female , Mice , Amino Acid Substitution , Animals , Bacterial Toxins/toxicity , Bacterial Toxins/metabolism , Bacterial Toxins/immunology , Bacterial Toxins/genetics , CHO Cells , Cyclic AMP/metabolism , Enterotoxins/toxicity , Enterotoxins/metabolism , Enterotoxins/immunology , Enterotoxins/genetics , Enzyme-Linked Immunosorbent Assay , Escherichia coli/metabolism , Escherichia coli/genetics , Cricetinae , Immunoglobulin A, Secretory/blood , Ileum/metabolism , Immunity, Mucosal , Mice, Inbred BALB C , Mutagenesis, Site-Directed , ADP Ribose Transferases/metabolism , Recombinant Proteins/toxicity , Recombinant Proteins/metabolism , Recombinant Proteins/immunology , Recombinant Proteins/chemistryABSTRACT
Objetivo: describir la relación entre las concentraciones normales de IgAs y la albúmina salival en niños normales. Material y método: se midieron los concentraciones de IgAs, albúmina y razón 19AS/albúmina en saliva de 60 niños sanos de 0 a 9 años. Resultados: la concentración de IgAs no se distribuyó normalmente en la población estudiada y la distribución no se normalizó al corregir por albúmina. Los puntos de corte establecidos en base a los percentile, 2,5 y 97,5 en el conjunto de la muestra fueron 40,2 y 788 mg/L respectivamente, siendo el promedio de 239,03 m/L. No hubo diferencia estadísticamente significativa entre grupos por edad. Conclusiones: Aún no existe suficiente información sobre la utilidad clínica de la razón IgAs/albúmina, la cual debe evaluarse