ABSTRACT
ABSTRACT Introduction Skeletal muscle injuries account for 10% to 50% of treadmill sports injuries. Insulin-like growth factor (IGF) is a family of polypeptides with both insulin-like anabolic and growth-promoting effects. Sports play a vital role in the recovery of skeletal muscle injuries. Objective The paper analyzes the ability of insulin-like growth factor 1 (IGF-1) to repair skeletal muscle injury caused by treadmill exercise. Method We injected drugs under the wound after exercise-induced injury in rats. The control group was injected with saline, and the experimental group was injected with an insulin-like growth factor. We conduct histological and electron microscopic structural analysis of rats, Results: After an injury, the experimental group formed a basal lamina protective film earlier than the control group, activated myoblasts, formed myofilaments, formed myotubes, and fused into muscle fibers earlier than the control group. The healing quality was also better. The experimental group was endogenous. The mRNA content of sex IGF-1 and IGF-2 both increased earlier than the control group. Conclusion Local injection of exogenous insulin-like growth factor-1 can stimulate the proliferation of myoblasts and accelerate the post-traumatic repair process of skeletal muscle caused by treadmill sports. Level of evidence II; Therapeutic studies - investigation of treatment results.
RESUMO Introdução As lesões do músculo esquelético representam de 10% a 50% das lesões em esteira esportiva. O fator de crescimento semelhante à insulina (IGF) é uma família de polipeptídeos com efeitos anabólicos e de promoção do crescimento semelhantes à insulina. Os esportes desempenham um papel vital na recuperação de lesões musculares esqueléticas. Objetivo o artigo analisa a capacidade do fator de crescimento semelhante à insulina 1 (IGF-1) em reparar lesões musculares esqueléticas causadas por exercícios em esteira. Método Injetamos drogas sob a ferida após lesão induzida por exercício em ratos. O grupo controle foi injetado com solução salina e o grupo experimental foi injetado com um fator de crescimento semelhante à insulina. Realizamos análises histológicas e microscópicas eletrônicas estruturais de ratos. Resultados Após a lesão, o grupo experimental formou um filme protetor da lâmina basal mais cedo do que o grupo controle, mioblastos ativados, miofilamentos formados, miotubos formados e fundidos em fibras musculares mais cedo do que o grupo controle. A qualidade da cura também foi melhor. O grupo experimental era endógeno. O conteúdo do sexo IGF-1 e IGF-2 mRNA aumentou mais cedo do que no grupo de controle. Conclusão A injeção local de fator de crescimento semelhante à insulina 1 exógeno pode estimular a proliferação de mioblastos e acelerar o processo de reparo muscular esquelético pós-traumático causado por esportes em esteira. Nível de evidência II; Estudos terapêuticos: investigação dos resultados do tratamento.
RESUMEN Introducción Las lesiones del músculo esquelético representan del 10% al 50% de las lesiones deportivas en cinta. El factor de crecimiento semejante a la insulina (IGF) es una familia de polipéptidos con efectos anabólicos y estimulantes del crecimiento semejantes a la insulina. Los deportes juegan un papel vital en la recuperación de las lesiones del músculo esquelético. Objetivo El artículo analiza la capacidad del factor de crecimiento semejante a la insulina 1 (IGF-1) para reparar la lesión del músculo esquelético causada por el ejercicio en cinta. Método inyectamos drogas debajo de la herida después de una lesión inducida por el ejercicio en ratas. Al grupo de control se le inyectó solución salina y al grupo experimental se le inyectó un factor de crecimiento semejante a la insulina. Realizamos análisis estructurales histológicos y microscópicos electrónicos de ratas, Resultados: Después de una lesión, el grupo experimental formó una película protectora de la lámina basal antes que el grupo de control, activó mioblastos, formó miofilamentos, formó miotubos y se fusionó en fibras musculares antes que el grupo de control. La calidad de curación también fue mejor. El grupo experimental fue endógeno. El contenido de ARNm de IGF-1 e IGF-2 de sexo aumentaron antes que en el grupo de control. Conclusión La inyección local de factor de crecimiento semejante a la insulina 1 exógeno puede estimular la proliferación de mioblastos y acelerar el proceso de reparación postraumático del músculo esquelético causado por los deportes en cinta. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.
Subject(s)
Humans , Male , Rats , Wound Healing/drug effects , Insulin-Like Growth Factor I/administration & dosage , Muscle, Skeletal/injuries , Acute Disease , Muscle, Skeletal/drug effects , Muscle, Skeletal/ultrastructure , Disease Models, AnimalABSTRACT
Objetivou-se avaliar a condição metabólica e estrutural das células espermáticas bovinas após congelação, com adição prévia de IGF-I e insulina no meio diluidor seminal. Os ejaculados de seis touros Nelore foram submetidos a quatro tratamentos: controle; insulina (100µUI/mL); IGF-I (150ng/mL) e insulina + IGF-I (50µUI/mL e 75ng/mL, respectivamente). Após a congelação, realizaram-se os testes de termorresistência rápida, coloração pelo corante azul de tripan e Giemsa, além da análise computadorizada da motilidade espermática, da integridade das membranas plasmática e acrossomal, e da peça intermediária por meio de sondas fluorescentes. O teste de termorresistência rápida apresentou efeito dentro do tempo de cada tratamento, mas não entre os tratamentos. Na análise computadorizada da motilidade espermática, foram observados movimento, motilidade e velocidade espermáticos; não houve efeitos dos tratamentos sobre qualquer uma dessas variáveis. Respostas iguais foram obtidas com as sondas fluorescentes e o corante azul de tripan/Giemsa. A adição de insulina e IGF-I, de forma isolada ou combinada, ao meio diluidor para congelação de sêmen não produziu efeitos sobre as condições metabólica e estrutural das células espermáticas.(AU)
This study aimed to evaluate the metabolic and structural condition of the spermatic bovine cells after the freezing, with addition, previously, of IGF-I and Insulin in the seminal thinner medium. The semen of 6 Nellore bulls were submitted to four treatments: Control, Insulin (100µUI/mL); IGF-I (150ng/mL) and Insulin + IGF-I (50µUI/mL and 75ng/mL, respectively). After freezing, rapid resistance tests, Tripan and Giemsa Blue staining, and computerized analysis of sperm motility and integrity of the plasma and acrosomal membranes and the intermediate part were performed by fluorescent probes. The term rapid resistance test had effect within the time of each treatment, but not between treatments. In the computer analysis of sperm motility, sperm movement, motility and velocity no effects of treatments were observed on any of these variables. The same results were obtained with the fluorescent probes and the Blue dye Trypan / Giemsa. The addition of Insulin and IGF-I, alone or in combination, to the semen freezing dilution medium had no effect on the metabolic and structural condition of sperm cells.(AU)
Subject(s)
Semen/metabolism , Insulin-Like Growth Factor I/administration & dosage , Cryopreservation/veterinary , Insulin/administration & dosage , Cattle , Indicators and ReagentsABSTRACT
ABSTRACT PURPOSE: To investigate the effects of pharmacological delay with insulin-like growth factor-1 (IGF-1) on skin flap survival. METHODS: Thirty Sprague-Dawley rats were submitted to dorsal skin flap (3x9 cm). Seven days before the surgery, the animals were subdivided into three groups of 10 rats. In group 1 (controls), no injection was done. Seven days before the elevation, saline had been injected to the marked skin flap area in group 2 (sham group), and group 3 (experimental group) underwent a pharmacological delay with subcutaneous IGF-1 injections. On the seventh postoperative day, flap area was analyzed for survival. Tissue samples were obtained for histological and biochemical evaluations. RESULTS: Survival rates were 43.55 ± 16%, 21.40 ± 8%, and 43.12 ± 14% in groups 1, 2, and 3, respectively. Differences between group 2 and other groups were statistically significant. No significant difference was detected between all three groups for tissue or plasma vascular endothelial growth factor (VEGF) levels. There was no significant histological difference between groups. CONCLUSION: Although a single injection of insulin-like growth factor-1 (IGF-1) did not significantly increase flap survival, its wound healing features are still encouraging and further meticulously planned studies, especially with repeated applications or controlled-release methods, and combinations with binding protein are required.
Subject(s)
Animals , Rats , Surgical Flaps/physiology , Wound Healing/drug effects , Insulin-Like Growth Factor I/pharmacology , Graft Survival/drug effects , Anti-Inflammatory Agents/pharmacology , Surgical Flaps/blood supply , Insulin-Like Growth Factor I/administration & dosage , Rats, Sprague-Dawley , Vascular Endothelial Growth Factors/blood , Injections, Subcutaneous , Anti-Inflammatory Agents/administration & dosageABSTRACT
This work evaluated the bone-forming potential of the platelet-derived growth factor isoform BB (PDGF-BB), insulin-like growth factor I (IGF-I), and mixed PDGF-BB/IGF-I delivered in liposomes compared with phosphate buffered saline (PBS), in the healing process of rat tooth sockets. One hundred and twelve Wistar rats were randomized into 7 groups of 16 animals each and were evaluated at 3, 7, 14 and 21 days after extraction of the maxillary second molars. The left sockets were treated with PBS (P), empty liposome (L), IGF-I in PBS (IP), IGF-I in liposome (IL), PDGF-BB in PBS (PDP), PDGF-BB in liposome (PDL) and both growth factors (GFs) together within liposomes (PDIL). The right sockets were filled with blood clot (BC). Histological and histomorphometric analyses were used to evaluate the formation of new bone and blood vessels. Immunohistochemistry was performed to evaluate the expression of osteocalcin and vascular endothelial growth factor (VEGF) during bone repair. Data were tested statistically using a Tukey's test according to a Dunn's analysis and Mann-Whitney U test followed by Kruskal-Wallis one-way analysis. Results were considered significant when p<0.05. A significantly higher percentage of bone trabeculae and a higher number of blood vessels were observed in the IL, PDL and PDIL groups (p<0.05). However, these GF-liposome groups had statistically similar results. Immunohistochemical assays first detected osteocalcin and VEGF expression at 3 days followed by a peak at 7 days. Lower immunoreactivity levels were observed in the BC, L, P, IP and PDP groups compared with the IL, PDL and PDIL groups (p<0.05). The results suggest that GFs carried by liposomes, either in isolated or mixed forms, enhanced the healing process in rat tooth sockets. The differential expression of the osteogenic markers VEGF and osteocalcin in the early phases of bone healing support these findings.
Este trabalho avaliou o potencial de formação óssea do fator de crescimento derivado de plaquetas na isoforma BB (PDGF-BB), fator de crescimento semelhante à insulina I (IGF-I), e a mistura PDGF-BB/IGF-I administrada em lipossomas comparando com tampão fosfato salino (PBS), no processo de cicatrização de alvéolos dentários de ratos. Cento e doze ratos Wistar foram distribuídos aleatoriamente em 7 grupos de 16 animais cada e foram avaliados aos 3, 7, 14 e 21 dias após a extração dos segundos molares maxilares. Os alvéolos esquerdos foram tratados com PBS (P), lipossomas vazios (L), IGF-I em PBS (IP), IGF-I em lipossomas (IL), PDGF-BB em PBS (PDP), PDGF-BB em lipossomas (PDL) e ambos os fatores de crescimento (GFs) em associação dentro de lipossomas (PDIL). Os alvéolos direitos foram preenchidos com coágulo sanguíneo (BC). As análises histomorfométrica e histológica foram utilizadas para avaliar a formação de novo osso e vasos sanguíneos. Imunohistoquímica foi realizada para avaliar a expressão de osteocalcina e o fator de crescimento endotelial vascular (VEGF) durante o reparo ósseo. Os dados foram testados estatisticamente utilizando o teste de Tukey em acordo com análise de Dunn e o teste Mann-Whitney U seguido pela análise de um passo de Kruskal-Wallis. Os resultados foram considerados significantes quando p<0,05. Uma percentagem altamente significativa de osso trabecular e alto número de vasos sanguíneos foram observados nos grupos IL, PDL e PDIL (p<0,05). Todavia, esses grupos lipossoma-GF tiveram resultados similares estatisticamente. Ensaios de imunohistoquímica inicialmente detectaram a expressão de osteocalcina e VEGF aos 3 dias, seguida por um pico aos 7 dias. Niveis mais baixos de imunorreatividade foram observados em BC, L, P, PI e PDP quando comparados com os grupos IL, PDL e PDIL (p<0,05). Os resultados sugerem que GFs carreados por lipossomas, na forma isolada ou em combinação, aceleram o processo de cicatrização em alvéolos dentários de rato. A expressão diferencial dos marcadores osteogênicos VEGF e osteocalcina, nas fases iniciais de cicatrização óssea, confirma esses achados.
Subject(s)
Animals , Male , Rats , Insulin-Like Growth Factor I/administration & dosage , Liposomes , Proto-Oncogene Proteins c-sis/administration & dosage , Tooth Socket/pathology , Wound Healing/drug effects , Insulin-Like Growth Factor I/pharmacology , Proto-Oncogene Proteins c-sis/pharmacology , Rats, WistarABSTRACT
O fator de crescimento insulina-simile(IGF-1) e um importante agente mitotico, com propriedades semelhantes as da insulina, que estimula o crescimento de celulas de varios tecidos tais como os osteoblastos, e celulas neoplasicas, inclusive as do cancer de mama...
Subject(s)
Humans , Female , Insulin-Like Growth Factor I/administration & dosage , Menopause , Hormone Replacement Therapy/methods , Bone Density/physiology , /administration & dosageABSTRACT
The biological action of insulin like growth factor-1 (IGF-1) on follicular steroidogenesis during follicular development in common carp was examined. Studies were carried out by culturing small (1-2 mm diam.) and large (> 2 mm diam.) follicles. IGF-1 (0.3-100 ng/ml) had no effect on progesterone accumulation or aromatase activity during 48 hr culture of small follicles. Progesterone accumulation by large follicles was also unaffected by IGF-1 over the same period, although aromatase activity was stimulated in a dose dependent manner (8-fold increase over basal levels with a maximum stimulatory dose of 30 ng IGF-1/ml). In contrast, small and large follicles responded to IGF-1 in terms of both progesterone accumulation and aromatase activity after longer periods of culture (4 days for progesterone and 6 days for aromatase). Concurrent treatment of small follicles with estradiol (10(-7) M) enhanced the action of IGF-1 on both indices of steroidogenesis and advanced the time at which IGF-1 stimulated activity was first detectable. The effect of estradiol on follicular IGF-1 responsiveness were independent of cell number. In summary, these results demonstrate varied actions of IGF-1 carp ovarian follicular steroidogenesis in vitro. The results indicate that carp follicles acquire responsiveness to IGF-1 in terms of aromatase activity during follicular development in vivo and that estradiol can induce the response in vitro. The results also suggest that estrogen and progesterone biosynthesis by cultured carp ovarian follicles is differentially regulated by IGF-1. Together, these results provide new insights into the biological actions of IGF-1 in fish ovary.
Subject(s)
Animals , Aromatase/metabolism , Culture Techniques , Estradiol/administration & dosage , Estrogens/biosynthesis , Female , Humans , Insulin-Like Growth Factor I/administration & dosage , Ovarian Follicle/drug effects , Progesterone/biosynthesis , Recombinant Proteins/administration & dosage , Steroids/biosynthesisABSTRACT
Luego de la resección intestinal masiva el intestino remanente experimenta cambios mucosos muy importantes para compensar la remoción de la superficie absortiva. Estos cambios pueden ser disminuidos aportando nutrientes enterales y factores del crecimiento exógenos tales como el IGF-1. La adaptación colónica puede ser aumentada mediante la adición de una mejora en la absorción del intestino delgado. Existe evidencia experimental preliminar de que el aporte intraluminar de IGF-1 produce efectos tróficos directos sobre el intestino delgado, probablemente mediados por mecaniamos paracrinos.
Subject(s)
Humans , Insulin-Like Growth Factor I/administration & dosage , Insulin-Like Growth Factor I/biosynthesis , Insulin-Like Growth Factor I/pharmacokinetics , Insulin-Like Growth Factor I/physiology , Insulin-Like Growth Factor I/standards , Insulin-Like Growth Factor I/therapeutic use , Short Bowel Syndrome/rehabilitation , Short Bowel Syndrome/therapyABSTRACT
A resposta catabólica ao trauma está associada à desnutriçäo mesmo com aporte proteico adequado. A associaçäo de fatores de crescimento ao suporte nutricional, parenteral e enteral, pode diminuir a fase catabólica do estresse em doenças crônicas e no período pós-operatório. Dentre oas fatores de crescimento, o hormônio de crescimento e o fator de crescimento insulina 1 têm particular açäo no metabolismo intermediário, podendo ser utilizados em determinadas situaçöes clínicas e de interesse cirúrgico