1.
Journal of Forensic Medicine
; (6): 129-131, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-982943
ABSTRACT
OBJECTIVE@#To observe the means of fibronectin(FN) alternative splicing and the expression of EIIIA-FN variant in rat skin after bruise, for the sake of providing some help for forensic estimation of wound interval.@*METHODS@#Total RNA was isolated from wounded skin, and reverse transcription polymerase chain reaction was conducted to amplify target segments.@*RESULTS@#Detectable EIIIA+(526 bp) segments, lacked in normal organize, was amplified at 1 h after experimental wound, and the levels were increased within 24 h.@*CONCLUSION@#The alternative splicing EIIIA-fibronectin variant would be a satisfied criterion for research of skin injury.