ABSTRACT
Microbial siderophores confiscate the available ferric ions around the roots and trigger a reaction resulting in plant growth promotion. In our study, a high level of siderophore production was observed from a newly isolated Pseudomonas sp. from the rhizosphere of Chickpea plants. Under an iron depleted condition in Standard Succinic acid medium a 1000 µgmL-1 of siderophore production was achieved. Increasing the concentration of iron showed an inverse relationship between growth and siderophore production. Fourier Transform Infrared Spectroscopy (FTIR) analysis of the purified crystals, its UV spectral analysis and High Pressure Liquid Chromatography (HPLC) revealed the identity of the siderophore as similar to that of pyoverdin with distinctive characters. Electron spray ionization mass spectroscopy (ESIMS) shows presence of abundance of A1 ions (419 m/z) and branching of amino acids from B1-B5. This pyoverdin contains a cyclic tetra peptide but Serine and Arginine are missing. Based on our analysis and deviations from the reported structure of pyoverdin it is suggested that this pseudomonas produces distinctly characterized pyoverdin siderophore.
Subject(s)
Succinic Acid/analysis , Succinic Acid/isolation & purification , Aeromonas/isolation & purification , Iron Compounds/analysis , In Vitro Techniques , Plant Structures , Siderophores/analysis , Siderophores/isolation & purification , Chromatography, High Pressure Liquid/methods , Fluorescence , MethodsABSTRACT
We assessed the quality of partially treated drinking water in 30 private establishments in Sana'a City, Republic of Yemen. We also compared the assessed water with the quality of 43 private wells and 18 estate wells. Microbiological examinations showed that 83% of the samples were contaminated; 50% with fecal coliforms and 33% with total coliforms. Concentration of mineral exceeded nominal values in 7% of the samples for nitrates, 10% for iron salts and in 20% of the samples for fluorides. In 33% of the samples, fluoride concentrations were lower than normal. Only 16.7% of the samples were found microbiologically and chemically potable
Subject(s)
Humans , Colony Count, Microbial , Enterobacteriaceae/isolation & purification , Fluorides/analysis , Iron Compounds/analysis , Minerals/analysis , Urban Health/statistics & numerical data , Water Pollutants, Chemical/analysis , Water Supply/analysisABSTRACT
Se determina hierro sérico, capacidad total de fijación de hierro, capacidad latente de fijación de hierro y por ciento de saturación de la transferrina en 30 vegetarianos y en 18 no vegetarianos hombres y mujeres adultos. No se han encontrado diferencias estadísticamente significativas para los exámenes realizadas en vegetarianos. Sin embargo el reducido número de muestras realizadas no permite dar una conclusión definitiva.